Objectif A central tenet of cortical operations is that excitation and inhibition rise in tandem through out a column, and over the somatic and dendritic compartments of single neurons. The details of how excitation flows in a column, how inhibition arises in different lamina to balance excitation, and how excitation and inhibition interact along the dendrite of pyramidal neurons – especially in vivo -- are not known. Our specific aims are to measure activity in all lamina of cortex, to measure activity of interneurons in all layers, and to directly measure the effect of inputs, activity, and behavioral state on dendritic activation and somatic output of interneurons and layer V pyramidal neurons. We will deploy 2-photon imaging methods for measuring state dependent changes in activity in layers 1-6, of somatosensory forepaw and barrel cortex, from known classes of neurons, and from the soma and dendrites of layer V pyramidal neurons in awake behaving, and anesthetized animals. The guiding hypothesis of this proposal is that excitation will be matched by inhibition in all cortical lamina, and during all states. A second simple hypothesis that is that inputs to apical dendrites will be amplified in all layer V neurons that fire action potentials. We will test these hypothesis during spontaneous activity, sensory stimulation and behavior. These studies will provide fundamental insights into cortical function. Programme(s) FP7-PEOPLE - Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Thème(s) FP7-PEOPLE-2013-IIF - Marie Curie Action: "International Incoming Fellowships" Appel à propositions FP7-PEOPLE-2013-IIF Voir d’autres projets de cet appel Régime de financement MC-IIF - International Incoming Fellowships (IIF) Coordinateur HUMBOLDT-UNIVERSITAET ZU BERLIN Contribution de l’UE € 223 778,40 Adresse UNTER DEN LINDEN 6 10117 Berlin Allemagne Voir sur la carte Région Berlin Berlin Berlin Type d’activité Higher or Secondary Education Establishments Contact administratif Renate Ubachs (Ms) Liens Contacter l’organisation Opens in new window Site web Opens in new window Coût total Aucune donnée
