Objetivo 50 years after the discovery of the DNA double helix, the variety of structures that nucleic acids can adopt continues to surprise the scientific community. Specific structures and conformational changes are linked to important functions in cell regulation. Understanding the principles that govern how small molecules such as natural metabolites or synthetic drugs modulate the nucleic acid structures is of prime importance for molecular biology and pharmacology. The field however suffers from the lack of suitable experimental tools to monitor all assemblies and structures formed when a small molecule encounters its targets.The goal of my project is to develop unique mass spectrometry-based approaches to detect, quantify and characterize all these assemblies and structures. Our team’s strength will be to integrate a multidisciplinary approach, from physical and analytical chemistry to molecular biology. We will address the fundamentals of nucleic acid ionization and transfer in the gas phase, develop a unique instrumental setup combining mass spectrometry, ion mobility and circular dichroism ion spectroscopy, and apply these new approaches to biologically important nucleic acids, in order to reveal the mechanisms of ligand-induced conformational changes in important regulatory structures such as G-quadruplex or riboswitches.This research will also have broader impact, as the approaches and concepts developed here for nucleic acids will contribute fundamental advances in mass spectrometry, and will be transferrable to other supramolecular or biological complexes. Ámbito científico natural sciencesbiological sciencesbiochemistrybiomoleculesnucleic acidsnatural sciencesbiological sciencesgeneticsDNAnatural scienceschemical sciencesanalytical chemistrymass spectrometrynatural sciencesphysical sciencesopticsspectroscopynatural sciencesbiological sciencesmolecular biology Programa(s) FP7-IDEAS-ERC - Specific programme: "Ideas" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Tema(s) ERC-CG-2013-PE4 - ERC Consolidator Grant - Physical and Analytical Chemical Sciences Convocatoria de propuestas ERC-2013-CoG Consulte otros proyectos de esta convocatoria Régimen de financiación ERC-CG - ERC Consolidator Grants Institución de acogida INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE Aportación de la UE € 2 021 755,20 Dirección RUE DE TOLBIAC 101 75654 Paris Francia Ver en el mapa Región Ile-de-France Ile-de-France Paris Tipo de actividad Research Organisations Investigador principal Valérie Gabelica (Dr.) Contacto administrativo Jérémy Bonnassies (Mr.) Enlaces Contactar con la organización Opens in new window Sitio web Opens in new window Coste total Sin datos Beneficiarios (1) Ordenar alfabéticamente Ordenar por aportación de la UE Ampliar todo Contraer todo INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE Francia Aportación de la UE € 2 021 755,20 Dirección RUE DE TOLBIAC 101 75654 Paris Ver en el mapa Región Ile-de-France Ile-de-France Paris Tipo de actividad Research Organisations Investigador principal Valérie Gabelica (Dr.) Contacto administrativo Jérémy Bonnassies (Mr.) Enlaces Contactar con la organización Opens in new window Sitio web Opens in new window Coste total Sin datos
