CORDIS

Literature review report on the monitoring of biological effects of oil spills and oil spill responses in the northern Atlantic and Baltic Sea

Report, baseline and database on influence of latitude and season on adverse outcome links in northern Atlantic and Baltic mussels after oil spills and oil spill responses

Report on results for natural removal of stranded oil on the coast in Arctic climatic regimes

Preparation and submission of scientifically articles in peer review journals based on results on in-situ burning of oil on sea ice.

Preparation, submission of abstract and presentation of in-situ burning of oil on sea ice results at conference.

Based on results of technological survey of D1.1 suitable oil detecting sensor will be tested on glider (underwater autonomous vehicle), which is increasingly important as cost-effective monitoring asset. Deliverable will analyse performance of oil detecting capability of specially equipped gliders, their possible use as oil detecting and monitoring tools especially in the Baltic Sea, giving beside horizontal also vertical dimension for oil spill detection and monitoring.

A dataset and report of biological effects and bioaccumulation of contaminants in field-collected and transplanted organisms in the area of the experimental oil spill after the oil response actions

Report on oil spill trajectory simulations according to the same range of selected scenarios for the Disko Bay and northern Baltic Sea areas as in D 5.2.

Deliverable analyses the bio-units developed to use zebrafish larvae until age 120 hours post fertilization as an alternative to animal testing. Biounit features a custom designed flow-through well plate made of polyamide, generated by selective laser sintering. To adapt the biounit to the specific parameters of the Baltic Sea influence of temperature and salinity on zebrafish larvae behaviour will be determined in accordance with similar experiments using zebrafish embryos. Thresholds for temperature and salinity tolerance of zebrafish larvae for behavioural monitoring will be determined.

The project management plan will contain a draft plan for time and places of consortium meetings, steering group meetings and advisory board meetings.

The dissemination and exploitation plan will contain an updated draft of planned publications, participation in conferences and other outreaching activities. It will also contain an overview of the products to be developed in GRACE and the plan for commercialization of these.

Taxonomic and functional characteristics of oil-degrading microbial community and oil biodegradation kinetic parameters in sea-ice interface are determined.

Based on results of meta-analysis of obtained omics data sets metagenomic prediction platform for inferring oil biodegradation activity in cold marine environment is established.

Deliverable discuss and analyse the probability of accidental spills in different sea areas and environmental conditions. Oil spill sensitivity mapping is important for coastal regions and sensitive sea in terms of the risk profile which changes over time with respect to changes in oil transportation pattern, legal instruments on ship design and operation, and new bunker oil qualities or alternative fuels. A structured overall spill risk assessment model for oil spills in Arctic and sub-arctic conditions is developed to identify response capacity needs, priority areas, and localization of resources.

Report on baselines and variability on latitudinal variability in biomarkers in mussels from the northern Atlantic, and associated database

Dataset and report on the biological effects and bioaccumulation of pollutants in an integrated pilot field study of a model oil spill and alternative oil spill responses

Report on simulations on vertical and horizontal oil spill dispersion according to a range of selected scenarios for the Disko Bay and northern Baltic Sea areas.

The dissemination and exploitation plan will contain a first draft of planned publications, participation in conferences and other outreaching activities. It will also contain an overview of the products to be developed in GRACE and the plan for commercialization of these.

A project brochure printed in colour with information on the project goals and participants with contact information will be printed and distributed to stakeholders.

Report including results from the baseline monitoring and baseline condition at test site for the field experiment.

Final version of sNEBA tool report launched on web pages.

Description of toxicity thresholds for acute and mechanism-specific effects as a measure of remediation success is provided.

Frame report for developed course in performing a sNEBA at PhD. level.

Report on oil in ice, including behaviour and burning, based on a review of the latest literature.

Test and evaluation of the fuzzy logic model output compared to a bivalent approach

Report on results from test of DESMI fire booms (Pyrobooms) for in situ burning of oil at shoreline.

Inventory of samples and data suitable for biological effects assessment in the northern Atlantic and Baltic Sea

Report including detailed test and mitigation plan, including approval for the pilot scale oil spill field experiment.

Matrix developed for input of environmental data of relevance for environmental assessment in connection with oil spill response with regard to the selected areas as in D 5.2.

Result report from the field experiments of environmental effects of situ burning, water wash and chemicals for shoreline clean-up, including toxic effect on littoral organisms.

First draft of sNEBA tool report based on the areas selected (Disko Bay and northern Baltic Sea) and previous deliveries.

Preparation and submission of the results from the field experiment in scientifically articles in peer review journals and as presentations at conferences.

Report on expected and missing environmental effects of the selected oils based on the PETROTOX model, and derivation of the impact on the experimental design

Report on adverse outcome links and transcriptomic fingerprinting for zebrafish regarding oil and dispersant contamination

Site specific trial application of the developed spill risk assessment methodology, including mapping the predicted probability of accidental oil spill.

Matrix developed with identification of operational requirements for oil spill response of relevance to the selected areas as in D 5.2.

Report on preliminary screening of design and data requirements based on input from relevant stakeholders, interviews obtained by skype, video link, telecon as well as physical meetings

Report on suitable environmental parameters for investigation of oil spills and oil spill responses using zebrafish

Report on classification of oil in ice based on latest knowledge on oil-ice interaction, ice types and oil properties.

Report on results for studies on natural removal of stranded oil on the coast in Arctic climatic regimes.

Deliverable focus on oil spill detection and monitoring from unmanned aerial vehicles (UAV) aiming at estimating the areal extent of the oil at sea surface in limited range from starting position and most probably used to widen monitoring capability of oil combatting or monitoring vessels. UAV technology becomes more and more affordable as flying platform, however sensors and systems could be used on UAV-s should be studied and tested together with drifters giving simultaneous ground truth information in certain point at sea. The main objective of UAVs is to provide operational information about (i) areal extent of oil spill and (ii) relative variability within the spill so that the combating could be planned/conducted in the best possible way.

Evaluation of effects of untreated versus treated oil on Calanus finmarchicus, and in relation to effects in Acartia tonsa and Arctic and Baltic zooplankton species

Deliverable present and discuss the SmartBuoy technology which turn conventional navigational buoys into platform for the environmental and oceanographic data collection and real time delivery. As navigation buoys are naturally located in key points of the fairways, SmartBuoys would provide the data about sea state exactly from where the vessels need it the most. Probability of oil spills is also highest on fairways, which motivates to equip SmartBuoys with oil detection sensors and having a real time early warning reporting system.

Oil-degrading microbial community structure in geographically different marine environments and impact of dispersant and environmental parameters on natural oil biodegradation kinetics is assessed and analysed.

Report on baselines and variability on seasonal and geographical variability in biomarkers in mussels from the Baltic Sea, and associated database

Deliverable analyses aspects of on-line detection and monitoring of oil spills is the main approach that we will contribute into the new system for oil spill response and search/development of sensors/platform technologies. Flow-through systems on board,Ships Of OPportunity (SOOP) so-called Ferryboxes, allow to enhance the system with oil detection capability. Relevant desing and test results of systems reporting the oil detection results in real time will be presented in the deliverable.

Deliverable deals with technology review and tests of available on market oil sensors is made and tests with selected sensors performed both in lab and field conditions. Prevailing measurement method of oil sensors commercially available today is the UV fluorescence, performance of every particular sensor technology is dependent on oil type to be detected and therefore related to fluorescence intensity in case of different wavelengths, will be analysed in deliverable.

Experimental technical report based on initial results from controlled in-situ burning experiments of oil on sea ice.

Report including results from test with DESMI and Lamor systems for collection of burn from the surface water after burning.

Workshop for relevant stakeholders; presentation of draft sNEBA tool for feedback, input and iterative process.

Project web pages including information on the consortium and the objectives of the project will be launched on the web site of SYKE

Oil remediation technology based on electrokinetic treatment is tested in situ and validated for different environmental conditions.

Toxicity assessment of a naphtenic North Sea crude oil using multi-level endpoints in zebrafish early live stages

Author(s): Sarah Johann, Leonie Nüßer, Vivian Götzen, Beat Thalmann, Andreas Schiwy, Henner Hollert and Thomas-Benjamin Seiler

Published in: Society of Environmental Toxicology and Chemistry Europe SETAC 2017 Abstract book, Issue 2017, 2017

In vitro toxicity testing in hemocytes of the marine mussel Mytilus galloprovincialis (L.) to uncover mechanisms of toxicity of the WAF of a naphthenic North Sea crude oil with and without dispersant

Author(s): A Katsumiti, G Nicolussi, D Bilbao, N Etxebarria, MP Cajaraville

Published in: 111th Iberian & 8th Ibero-american Congress of Environmental Contamination and Toxicology, Issue 11-13 July 2018, 2018

Temperature-dependent toxicity of Naphtenic North Atlantic crude oil WAF, dispersant and their mixture: sea urchin bioassays

Author(s): L de Miguel, U Izagirre, I Marigómez

Published in: SETAC Europe 28th Annual Meeting, Issue SETAC 2018. 13–17 May 2018, Rome, Italy, 2018

Can zebrafish larvae be used as a biosensor for contaminant detection in the Baltic Sea?

Author(s): S. Johann, L. Nüßer, H. Hollert & T.-B. Seiler

Published in: Fish and Amphibian Embryo as Alternative Models in Toxicology and Teratology, Issue 2016, 2016

Determination of oil spill and response impacts on biota using effect-based tools and ecological risk assessment – GRACE project WP3

Author(s): S. Johann, H. Hollert, L. Nüßer, K. Lehtonen, A. Ahvo, J. Nuutinen, R. Turj, S. Pärt, A. Reunam, I. Marigómez, M. Soto, N. Etxebarria, U. Izagirre, A. Olsen, B. Munro Jensen, I. Salaverria-Zabalegui, D. Alitin, T. Kouts & T.-B. Seiler

Published in: Society of Environmental Toxicology and Chemistry – Europe German Language Branch e.V. Abstract Book, Issue 2016, 2016

Operational in situ oil spill detection in the Baltic Sea, using FerryBox system equipped with oil sensor

Author(s): Kõuts T., Pärt S.., Vahter K.

Published in: IMDIS’2018 International Conference on Marine Data and Information Systems, Issue 2018, 2018

Bioaccumulation of PAH in mussels exposed to water accommodated fraction (waf) of oil at different conditions

Author(s): Bilbao D., de Miguel L., Olivares M., Aguirre-Rubi J., Izagirre U., Benito D., Lekube X., Marigomez I., Prieto A., Etxebarria N.

Published in: 40th International Conference on Environmental & Food Monitoring, Issue 19 - 22 June, 2018, 2018

Assessment of microbial community response to accidental release of oil into the Arctic marine ecosystem

Author(s): Marika Truu, Jaak Truu, Kristjan Oopkaup, Nga Dang, Anna Reunamo, Ossi Tonteri, Kirsten S. Jørgensen

Published in: CBM 2018 abstract book, 2018

Real time in situ oil-spill detection usingShips of Opportunity equipped with FerryBox system

Author(s): Pärt S., Kõuts T., Vahter K.

Published in: Oceanology International / Interspill 2018, Issue 2018, 2018

General stress biomarkers in mussels (Mytilus trossulus) collected in three different seasons from two regions of the Baltic Sea

Author(s): D Benito, I Marigómez, X Lekube, U Izagirre, B Zaldibar, M Soto, A Ahvo , KK Lehtonen

Published in: 11th Iberian & 8th Ibero-american Congress of Environmental Contamination and Toxicology, Issue 11-13 July 2018, 2018

Oil spill and response impacts on biota in cold climates – effect-based tools and ecological risk

Author(s): T.-B. Seiler, S. Johann, L. Nüßer, K. Lehtonen, A. Ahvo, R. Turja, A. Reunamo, J. Nuutinen, I. Marigómez, M. Soto, N. Etxebarria, U. Izagirre, A. Orbea, X. Lekube, E. Gil-Uriarte, A.J. Olsen, B.M. Jenssen, I. Salaberria, T.M. Ciesielski, D. Altin, T. Kõuts, S. Pärt, M. Duchemin, K. Jørgensen & H. Hollert

Published in: Society of Environmental Toxicology and Chemistry Europe SETAC 2017 Abstract book, Issue 2017, 2017

Zebrafish larvae behaviour as a biological early warning system for aquatic systems

Author(s): Leonie Nuesser, Sarah Johann, Elad Salomons, Olya Skulovich, Sarah Hartmann, Caroline Ganal Catrina Cofalla, Holger Schuettrumpf, Avi Ostfeld, Kirsten Jørgensen, Tarmo Kõuts, Henner Hollert and Thomas-Benjamin Seiler

Published in: Society of Environmental Toxicology and Chemistry Europe SETAC 2017 Abstract book, Issue 2017, 2017

Behavior of marine diesel oil in a semi-static mussel (Mytilus spp.) exposure experiment and observed biological effects.

Author(s): Raisa Turja, Aino Ahvo, Harri Kankaanpää, Anna Reunamo, Kari Lehtonen, Kirsten S. Jørgensen.

Published in: SETAC Europe 28th Annual Meeting, Issue SETAC 2018. 13–17 May 2018, Rome, Italy, 2018

Integrated oil spill response actions andenvironmental effects –GRACE

Author(s): Jørgensen K. S., Wegeberg S., Truu J., Kõuts T., Seiler T.-B., Marigomez I., Jenssen B. M., Petrich C., Wilms L. B., Forsman B., Wang F., Högström R., Virtanen S, Pärt S.

Published in: Baltic Earth Workshop on Multiple drivers for Earth system changes in the Baltic Sea region,, Issue 2018, 2018

"EU H2020 project GRACE: Experimental design for assessing the impact of oil and oil-dispersant exposure on Norwegian Sea and Baltic Sea mussels (Mytilus spp.) under ""natural"" conditions"

Author(s): X. Lekube, A. Ahvo, D. Altin, T.M. Ciesielski, E. Gil-Uriarte, U. Izagirre, K.S. Jørgensen, I. Marigómez, J. Nuutinen, M. Soto, R. Turja, K.K. Lehtonen

Published in: Society of Environmental Toxicology and Chemistry Europe SETAC 2017 Abstract book, Issue 2017, 2017

Impact of dispersants on petroleum hydrocarbon biodegradation and degradation gene abundances in the Baltic Sea.

Author(s): Ossi Tonteri, Aura Nousiainen, Anna Reunamo, Jari Nuutinen, Jaak Truu and Kirsten S. Jørgensen.

Published in: ISME 2018. Leipzig, Germany, Abstract book, Issue 17th International Symposium on Microbial Ecology 12 - 17 August 2018, 2018

Cellular and tissue-level biomarkers in mussels (Mytilus edulis) sampled in two different study areas in the Northern Atlantic

Author(s): D. Benito, U. Izagirre, X. Lekube, I. Marigomez, B. Zaldibar, M. Soto

Published in: SETAC Europe 28th Annual Meeting, Issue SETAC 2018. 13–17 May 2018, Rome, Italy, 2018

Toxicity of crude oil WAF assessed by bioassays in Hediste diversicolor

Author(s): S Blanco, L de Miguel, X Lekube, I Marigómez, M Soto, UIzagirre

Published in: 11th Iberian & 8th Ibero-american Congress of Environmental Contamination and Toxicology, Issue 11-13 July 2018, 2018

Assessment of the Trojan horse effect using PAH-contaminated polystyrene microplastics: sorption studies and responses of adult zebrafish.

Author(s): Martinez-Alvarez I, Le Menach K, Devier M-H, Cajaraville, MP, Budzinski H, Orbea A

Published in: Conference on Fate and Impact of Microplastics: Knowledge, Actions and Solutions., Issue 19-23 November 2018, 2018

Gentoxisches Potential von unbehandelten und chemisch dispergierten fossilen Ölen

Author(s): Mira Goßen, Sarah Johann, Henner Hollert, Thomas-Benjamin Seiler

Published in: Umwelt 2018, SETAC GLB/GDCh joint annual meeting Abstract book, 2018

The effect of three different arctic oil water accommodated fraction conditions on mortality and fecundity of the copepod Acartia tonsa

Author(s): T Hafez, M Ortiz-Zarragoitia.

Published in: 19th International Symposium on Pollutant Responses in Marine Organisms, Issue 30 June-3 July 2017, 2018

Toxicity to sea urchin embryos of WAFs produced at different temperatures with crude oils alone and mixed with dispersant

Author(s): L de Miguel, U Izagirre, X Lekube, I Marigómez

Published in: 11th Iberian & 8th Ibero-american Congress of Environmental Contamination and Toxicology, Issue 11-13 July 2018, 2018

Oil detection with ferrybox system: project GRACE

Author(s): Tarmo Kõuts*, Siim Pärt* and Leonie Nüsser** *Tallinn University of Technology ** Aachen University

Published in: BOOS Annual Meeting and Conference, Issue 2018, 2018

Integrated oil spill response actions and environmental effects –GRACE EU-H2020 project.

Author(s): Kirsten S. Jørgensen

Published in: Abstract book MOSPA conference, Issue International MOSPA 2018 event in Oulu 7-8 March, 2018

Adaptions of in-vitro bioassays for crude oil toxicity profiling

Author(s): Sarah Johann, Henner Hollert, Thomas-Benjamin Seiler

Published in: BioDetectors Conference 2018 Abstract book, Issue 2018, 2018

Cytotoxicity of the WAF of naphthenic North Sea crude oil with and without dispersant in hemocytes of the marine mussel Mytilus galloprovincialis (L.)

Author(s): G Nicolussi, A Katsumiti, D Bilbao, N Etxebarria MP Cajaraville

Published in: SETAC Europe 28th Annual Meeting, Issue SETAC 2018. 13–17 May 2018, Rome, Italy, 2018

Operational in situ oil spill detection in the Baltic Sea, using FerryBox system equipped with oil sensor

Author(s): Pärt S., Kõuts T., Vahter K.

Published in: 7th IEEE/OES Baltic Symposium, Issue 2018, 2018

Ferrybox system, as possibility to monitor oil compounds on fairways.

Author(s): Kõuts T., Pärt S.., Vahter K.

Published in: EuroGOOS International Conference: Operational Oceanography serving sustainable marine development, Issue 2017, 2017

Investigating the endocrine disruptive and genotoxic potential of crude oil samples using an adapted in vitro toxicity toolbox

Author(s): Sarah Johann, Leonie Nüßer, Lucie Spencer, Dag Altin, Henner Hollert, Thomas-Benjamin Seiler

Published in: SETAC Annual Meeting 2018 Abstract book, Issue SETAC 2018. 13–17 May 2018, Rome, Italy, 2018

Effect of oil exposure on the Baltic Sea mussel Mytilus trossulus microbiome.

Author(s): Anna Reunamo, Raisa Turja, Aino Ahvo, Jari Nuutinen, Kari K. Lehtonen, Kristjan Oopkaup, Jaak Truu, Kirsten S. Jørgensen.

Published in: ISME 2018. Leipzig, Germany, Abstract book, Issue 17th International Symposium on Microbial Ecology 12 - 17 August 2018, 2018

Impact of microplastics alone or with adsorbed oil compounds from the water accommodated fraction of a North Sea crude oil on marine mussels Mytilus galloprovincialis.

Author(s): González-Soto N, Katsumiti A, Duroudier N, Orbea A, Bilbao E, Navarro E, Cajaraville MP

Published in: Conference on Fate and Impact of Microplastics: Knowledge, Actions and Solutions., Issue 19-23 November 2018, 2018

Biomarkers of oxidative-stress in Calanus finmarchicus exposed to WAF from anaphthenic North Sea crude oil

Author(s): S.Solopertp, D. Altin, A. Hallmann, E. Skottene, B.H. Hansen, B.M. Jenssen, T.M. Ciesielski

Published in: Proceedings of the Norwegian Environmental Toxicology Symposium, Svalbard,, 2018

Author(s): Siim Pärt, Tarmo Kõuts, Kaimo Vahter

Published in: The 11th Baltic Sea Science Congress ‘Living along gradients: past, present, future’ Abstracts 2017, Issue 2017, 2017, Page(s) 333

DOI: 10.12754/procs-2017-bssc

Biomarker responses to chemically dispersed crude oil in the Baltic Sea sediment dwelling bivalve, Macoma balthica

Author(s): Aura Nousiainen, Raisa Turja, Aino Ahvo, Lisandra Alves, Kari Lehtonen, Kirsten S. Jørgensen

Published in: Society of Environmental Toxicology and Chemistry Europe SETAC 2017 Abstract book, Issue 2017, 2017

Real time in situ oil-spill monitoring using FerryBox system equipped with UV-fluorometer

Author(s): Pärt S., Kõuts T., Vahter K.

Published in: From small scales to large scales –The Gulf of Finland Science Days 2017, Issue 2017, 2017

Effects of naphthenic North Atlanticcrude oil on metabolome profile inCalanus finmarchicus

Author(s): Ewa Agnieszka Cichosz

Published in: MSc thesis in Environental Toxicology and Chemistry, 2018, Page(s) 75pp

In vitro toxicity profiling of the effects of oil and of oil remediation strategies on mussel Mytilus galloprovincialis hemocytes.

Author(s): Greta Nicolussi (Supervisor: MP Cajaraville)

Published in: MSc in Marine Biology, Issue Sept 2017, 2017

Effects of the water accommodated fraction of a Norwegian crude oil on Daphnia magna and chemical analysis of target PAHs and Alkanes

Author(s): Christian Neuser

Published in: Theses from the Institute for Environmental Research, RWTH Aachen University, Issue 2017, 2017

Assessment of the toxicity of crude oil and dispersant at different temperatures using zebrafish

Author(s): Nagore González-Soto (Supervisors: A Orbea)

Published in: MSc in Environmental Contamination and Toxicology, Issue Sept 2017, 2017

Effect of temperature on the responsiveness of tissue-level biomarkers in Norwegian mussels exposed to WAF and Dispersed WAF.

Author(s): Irati Ranero

Published in: MSc in Environmental Contamination and Toxicology, 2017

Analysis of gene expression altered by organic compounds in sea urchin embryos

Author(s): Helene Sanchez (Supervisors: O Diaz de Cerio, L de Miguel-Jimenez)

Published in: Biology BSc Degree Final Project, 2019, Page(s) in Basque / Spanish: Gene adierazpenaren analisia konposatu organikoen bidez alteratuko itsas trikuen larbetan / Análisis de expresión de genes alterados por compuestos orgánicos en embriones de erizos de mar

Biomarkers of oxidative stress in Calanusfinmarchicus exposed to a wateraccommodated fraction of a naphthenicNorth Atlantic crude oil

Author(s): Sofia Soloperto

Published in: MSc thesis in Environental Toxicology and Chemistry, 2018, Page(s) 75pp.

Crude oil WAF toxicity evaluation using Hediste diversicolor bioassays

Author(s): Sergio Blanco (Supervisors: U Izagirre, L de Miguel-Jimenez)

Published in: Biology BSc Degree Final Project, Issue Sept 2018, 2018, Page(s) in Spanish: Evaluación de la toxicidad de WAF de petróleo mediante bioensayos en Hediste diversicolor

Evaluation methods for effects of acute and chronic exposure of surface water-relevant contaminants on the swimming behaviour of Danio rerio early life stages

Author(s): Anne Kreutzer

Published in: Theses from the Institute for Environmental Research, RWTH Aachen University, Issue 2017, 2017

Sampling and passive dosing of Polycyclic Aromatic Hydrocarbons in sea water

Author(s): Amaia Igartua Rodriguez (Supervisor: N Etxebarria)

Published in: MSc in Environmental Contamination and Toxicology, Issue Sept 2017, 2017, Page(s) in Basque: hidrokarburo polizikliko aromatikoen biltze eta dosifikazio pasiboa itsas uretan.

Tissue-level biomarkers and histopathological alterations in mussels from the Baltic Sea exposed to Oil WAF and CEWAF in extreme brackish conditions

Author(s): Joyanta Bir

Published in: Erasmus Mundus Master in Marine Environment and Resources, 2017

Development of a method to analyse hydrocarbon by means of FT-IR and its applications

Author(s): Itsasne Beitia (Supervisors: N Etxebarria, D Bilbao)

Published in: Chemistry BSc Degree Final Project, Issue Sept 2017, 2017, Page(s) in Basque: T-IR-ren bidezko hidrokarburoak analizatzeko metodo baten garapena eta aplikazioak

Author(s): Alberto Katsumiti, Greta Nicolussi, Dennis Bilbao, Ailette Prieto, Nestor Etxebarria, Miren P. Cajaraville

Published in: Science of The Total Environment, Issue 670, 2019, Page(s) 1084-1094, ISSN 0048-9697

DOI: 10.1016/j.scitotenv.2019.03.187

Author(s): Bjørn Henrik Hansen, Anna Hallmann, Dag Altin, Bjørn Munro Jenssen, Tomasz M. Ciesielski

Published in: Journal of Toxicology and Environmental Health, Part A, 2017, Page(s) 1-10, ISSN 1528-7394

DOI: 10.1080/15287394.2017.1352182