Objectif - - To construct a cosmid/PAC/BAC map of the human X chromosome with reference clones accessible to all participants and based on highly annotated YAC maps.- - To isolate and map the majority of X chromosome transcripts to the cosmide pockets and when established, to the cosmid contigs.- - To perform large scale sequencing of selected regions of the X chromosome, principally Xq28 and Xp22.With the availability of near complete coverage of the human X chromosome in YAC contigs, the participants aim at a coordinated effort to construct a map of bacterial vectors, as a substrate to the isolation of most of the transcripts of the chromosome. For both objectives, two different and complementary strategies will be used. First a global and systematic approach will be used by the coordinator. A minimal tiling path of YAC DNA will be isolated by long-range Alu-PCR and screened against a variety of reference cosmid, PAC and BAC libraries, thus constructing a pocket map of the chromosome. A cDNA library enriched for X chromosome transcripts will be constructed by laboratory in Heidelberg and made available to all participants. It will be systematically screened with cosmids from the pocket map to position transcripts. Secondly, a more regional approach will be used by key participants on the X chromosome, who have accumulated large amounts of material and expertise. The same cosmid libraries and selected cDNA library will be made available to all, and this will allow an efficient pooling of results. To this end a core database will be maintained by the coordinator and accessible by all participants through local copies of the same dataset. The complete network will be constructed with the IGD system, based on the ACEDB graphical database program. Large scale sequencing using state of the art technologies and strategies will be performed in areas where sequence ready maps are already available. This will be mainly performed in Xq28 which has been shown to contain an unusually high number of genes, many of which related to genetic disorders. Champ scientifique natural sciencescomputer and information sciencesdatabasesnatural sciencesbiological sciencesgeneticsDNAnatural sciencesbiological sciencesgeneticschromosomes Programme(s) FP4-BIOMED 2 - Specific research, technological development and demonstration programme in the field of biomedicine and health, 1994-1998 Thème(s) 5.1 - Gene mapping and analysis Appel à propositions Data not available Régime de financement CSC - Cost-sharing contracts Coordinateur CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE Contribution de l’UE Aucune donnée Adresse Rue Laurent Fries 1 67404 ILLKIRCH GRAFFENSTADEN France Voir sur la carte Coût total Aucune donnée Participants (6) Trier par ordre alphabétique Trier par contribution de l’UE Tout développer Tout réduire Comitato Promotore Telethon Italie Contribution de l’UE Aucune donnée Adresse Via Olgettina 58 20132 Milano Voir sur la carte Coût total Aucune donnée Institut Pasteur France Contribution de l’UE Aucune donnée Adresse 25 rue du Docteur Roux 75015 Paris Voir sur la carte Coût total Aucune donnée MAX-PLANCK-GESELLSCHAFT ZUR FOERDERUNG DER WISSENSCHAFTEN E.V. Allemagne Contribution de l’UE Aucune donnée Adresse Ihnestrasse 73 14195 BERLIN Voir sur la carte Coût total Aucune donnée N/A Finlande Contribution de l’UE Aucune donnée Adresse 3,Haartmaninkatu 00014 Helsinki Voir sur la carte Coût total Aucune donnée UNIVERSITY COLLEGE LONDON Royaume-Uni Contribution de l’UE Aucune donnée Adresse Mortimer Street The Middlesex Hospital W1N 8AA London Voir sur la carte Coût total Aucune donnée University College Galway Irlande Contribution de l’UE Aucune donnée Adresse University Road 90 Galway Voir sur la carte Coût total Aucune donnée
