Objectif The final goals of the project are the establishment of novel markers for neuronal cell death (apoptosis) and the development of novel strategies to reduce ischemic neuronal damage in stroke patients (with special reference to the NMDA receptor antagonist Flupirtine). The specific aims of this project are: to study the expression of reliable markers for apoptosis in brain areas which are particularly sensitive to ischemia and in an area poorly sensitive to ischemia: cell biological markers (morphology) and molecular biological markers (expression of c-fos, c-jun, ubiquitin, hsp70); to investigate the hypoxic neuronal injury at the molecular level (studies in cultured neurons and studies in animal model); therapy-related studies of the neuroprotective effect of Ca2+channel blockers, NMDA receptor antagonists, radical scavengers. Preliminary results have revealed that selective NMDA receptor antagonists display a strong neuroprotective effect against ischemic cell damage. One compound turned out to be highly promising: Flupirtine, a centrally-acting non-opioid analgesic which was found to act as a NMDA receptor antagonist. In addition, hypoxic brain injury was found to be associated with changes in the ratio of quinolinic acid to kynurenic acid. Additional tasks of the project are: investigation of the neuroprotective effect of the newly discovered NMDA receptor antagonist Flupirtine; establishment of whether quinolinic acid is a suitable biochemical parameter which can be used as a measure for a therapy success during treatment of ischemia; establishment of a RIA for quantification of quinolinic acid. Programme(s) IC-INTAS - International Association for the promotion of cooperation with scientists from the independent states of the former Soviet Union (INTAS), 1993- Thème(s) 44 - Biotechnology Appel à propositions Data not available Régime de financement Data not available Coordinateur Johannes Gutenberg Universität Mainz Contribution de l’UE Aucune donnée Adresse Duesbergweg 6 55099 Mainz Allemagne Voir sur la carte Coût total Aucune donnée Participants (4) Trier par ordre alphabétique Trier par contribution de l’UE Tout développer Tout réduire Belarussian Research Institute of Epidemiology and Microbiology Biélorussie Contribution de l’UE Aucune donnée Adresse 220050 Minsk Voir sur la carte Coût total Aucune donnée Hellenic Anticancer Institute Grèce Contribution de l’UE Aucune donnée Adresse 11522 Athens Voir sur la carte Coût total Aucune donnée National Academy of Sciences of Ukraine Ukraine Contribution de l’UE Aucune donnée Adresse 252627 Kiev Voir sur la carte Coût total Aucune donnée Università di Camerino Italie Contribution de l’UE Aucune donnée Adresse 62032 Camerino Voir sur la carte Coût total Aucune donnée
