Objectif Weapons of bioterrorism, such as the toxin-producing bacteria Bacillus anthracis and Clostridium botulinum, pose serious threats to Western societies. Since no products are available that allow rapid vaccination, exposed populations would be completely unp rotected. Even if a vaccine could be developed, active immunization would require multiple doses. Thereafter, it would take many months to achieve protective levels of antibodies and vaccination could only be applied to selected risk-groups. We have recent ly developed a model system in which Lactobacillus, a GRAS microorganism, can be transformed with antibody fragment encoding vectors. This allows the expression of functional single- chain antibodies against mucosal pathogens in situ. These antibody fragme nts retain their biological properties in vivo and may mitigate or prevent disease. The aim of this project is to select and characterize Lactobacilli with different tropisms and to develop the vector system currently used into a food-grade transformation/ integration/cleaning system that allows simultaneous expression of multiple antibody specificities. Furthermore, we intend to clone the genes encoding the variable region from hybridomas producing neutralizing antibodies against protective antigen (B. anth racis) and botulinum toxins (C. botulinum). These will be introduced into the selected Lactobacilli and the resulting transformants tested for their protective capacity in animal models. Our approach, which falls outside the domains of the FP6 thematic pri orities, represents a completely novel system for the induction of passive immunity that can be rapidly applied to populations at risk (for example through the drinking water). The transformed Lactobacilli will colonize the mucosa in exposed individuals, a llowing production of protective antibody fragments in situ. If successful, this project could be applied to therapy against a vast number of human/animal pathogens # Champ scientifique engineering and technologyenvironmental engineeringwater treatment processesdrinking water treatment processessocial sciencespolitical sciencespolitical transitionsterrorismnatural sciencesbiological sciencesmicrobiologybacteriologymedical and health sciencesbasic medicineimmunologyimmunisationmedical and health sciencesbasic medicinepharmacology and pharmacypharmaceutical drugsvaccines Mots‑clés Bioterrorism GRAS microorganisms passive immunity Programme(s) FP6-POLICIES - Policy support: Specific activities covering wider field of research under the Focusing and Integrating Community Research programme 2002-2006. Thème(s) NEST-2003-1 - Adventure activities Appel à propositions FP6-2003-NEST-A Voir d’autres projets de cet appel Régime de financement STREP - Specific Targeted Research Project Coordinateur KAROLINSKA INSTITUTET Contribution de l’UE Aucune donnée Adresse Nobels väg 1 STOCKHOLM Suède Voir sur la carte Liens Site web Opens in new window Coût total Aucune donnée Participants (3) Trier par ordre alphabétique Trier par contribution de l’UE Tout développer Tout réduire CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS Espagne Contribution de l’UE Aucune donnée Adresse Serrano 117 MADRID Voir sur la carte Liens Site web Opens in new window Coût total Aucune donnée LACTRYS BIOPHARMACEUTICALS BV Pays-Bas Contribution de l’UE Aucune donnée Adresse Zernikedreef 9 LEIDEN Voir sur la carte Coût total Aucune donnée UNIVERSITY OF TARTU Estonie Contribution de l’UE Aucune donnée Adresse Uelikooli Str. 18 TARTU Voir sur la carte Liens Site web Opens in new window Coût total Aucune donnée
