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ASIARESIST Report Summary

Project ID: ICA4-CT-2001-10028
Funded under: FP5-INCO 2
Country: Malaysia

Isolation of chloramphenicol resistant bacteria from aquaculture farms in Malaysia

Isolation of chloramphenicol resistant bacteria from aquaculture farms in Malaysia.

Antimicrobial agents have been widely used in aquaculture worldwide to treat infections caused by a variety of bacterial pathogens. This study undertaken is the evidence of the occurrence of antibiotic-resistant bacteria specifically chloramphenicol resistant bacteria in the local aquaculture ecosystem. Sampling for the chloramphenicol resistant bacteria was conducted at three farms, two of which were fish farms (Tilapia integrated with Chicken Farm and Big Head Carp Farm) in the State of Perak and one shrimp farm (Banting Shrimp Farm) in the State of Selangor.

The farms were selected for sampling after investigations revealed higher than 35 ppm chloramphenicol resistant isolates. At each site, sampling was performed four times at 2 weeks interval. During each sampling the following data were recorded, depth at which water and sediment samples were taken, physico-chemical parameters of the water (including temperature, pH, salinity, total ammonia nitrogen (N-NH4) and total suspension solid). The sediment samples were described according to the soil type (clay, sandy, loam, mixed). As a result, a total of 166 chloramphenicol-resistant strains were successfully isolated according to the SOP guidelines.

This resistance can disseminate through the environment and can be transmitted to a variety of bacterial species, including bacteria that can infect humans. Bacteria, present in aquaculture settings may be transmitted to humans who come in contact with this ecosystem. Hence, alternative steps could be introduced, such as probiotics, vaccines and good aquaculture practices to control bacterial infections as this option could prove to be useful in our aquaculture since the prolonged use of antibiotics may result in selection of antibiotic-resistant bacteria.

Reported by

Universiti Putra Malaysia
Universiti Putra Malaysia
43400 Serdang
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