Cel The requirement to screen for known SNPs (single nucleotide polymorphisms) has become one of the key challenges to be addressed to enable the exploitation of the human genome sequence, where approximately 3 million SNPs are responsible for all of the variation within the human population. Several methods and technologies are currently available for detection of SNPs, but no single platform exists which can deliver 100% accuracy, in a low cost, versatile, and easy-to-use integrated system.The proposed research will focus on the development of integrated SNP detection platforms to include modules for DNA extraction and purification from biological samples, DNA amplification, DNA characterisation (including SNP detection), signal transduction, interpretation and data analysis. Integration of the modules on a single platform will include a transport mechanism based on electrowetting actuation (EWOD).Two alternatives SNP detection platforms will be developed based on optical and magnetic sensing respectively. The whole system will be packaged as a single automated functional system with a simplified GUI interface, based on specifications recommended by potential end-users within the consortium. This systems development will focus on delivering a product customised for low to medium throughput, low cost, point-of-care applications, with emphasis on providing very rapid and accurate results.Within the project, the systems will be benchmarked for screening of SNPs in the CFTR gene that are associated with cystic fibrosis. CF patient samples will be used to verify the accuracy and reproducibility of the system in a clinical diagnostics laboratory. Dziedzina nauki engineering and technologyelectrical engineering, electronic engineering, information engineeringelectronic engineeringcontrol systemsengineering and technologyelectrical engineering, electronic engineering, information engineeringelectronic engineeringsensorsoptical sensorsmedical and health sciencesbasic medicinepharmacology and pharmacypharmaceutical drugsnatural sciencesbiological sciencesgeneticsDNAnatural sciencesbiological sciencesgeneticsnucleotides Słowa kluczowe Nanotechnology Program(-y) FP6-NMP - Nanotechnologies and nanosciences, knowledge-based multifunctional materials and new production processes and devices: thematic priority 3 under the 'Focusing and integrating community research' of the 'Integrating and strengthening the European Research Area' specific programme 2002-2006. Temat(-y) NMP-2003-3.4.1.5-2 - Ethical, legal, social aspects of research in nanotechnology NMP-2004-IST-NMP-2 - Bio-sensors for Diagnosis and Healthcare Zaproszenie do składania wniosków FP6-2004-IST-NMP-2 Zobacz inne projekty w ramach tego zaproszenia System finansowania STREP - Specific Targeted Research Project Koordynator UNIVERSITY COLLEGE CORK, NATIONAL UNIVERSITY OF IRELAND, CORK Wkład UE Brak danych Adres Lee Maltings, CORK Irlandia Zobacz na mapie Linki Strona internetowa Opens in new window Koszt całkowity Brak danych Uczestnicy (6) Sortuj alfabetycznie Sortuj według wkładu UE Rozwiń wszystko Zwiń wszystko ASPER BIOTECH LTD Estonia Wkład UE Brak danych Adres Oru 3 TARTU Zobacz na mapie Linki Strona internetowa Opens in new window Koszt całkowity Brak danych CHARLES UNIVERSITY PRAGUE Czechy Wkład UE Brak danych Adres Ovocny TRH 5 PRAGUE 1 Zobacz na mapie Linki Strona internetowa Opens in new window Koszt całkowity Brak danych COMMISSARIAT A L'ENERGIE ATOMIQUE (CEA) Francja Wkład UE Brak danych Adres 31-33 rue de la Fédération PARIS Zobacz na mapie Linki Strona internetowa Opens in new window Koszt całkowity Brak danych FUNDACAO DA FACULDADE DE CIENCIAS DA UNIVERSIDADE DE LISBOA Portugalia Wkład UE Brak danych Adres Campo Grande - Edificio C7 - 1 piso LISBOA Zobacz na mapie Linki Strona internetowa Opens in new window Koszt całkowity Brak danych INSTITUTO DE ENGENHARIA DE SISTEMAS E COMPUTADORES PARA OS MICROSISTEMAS E AS NANOTECNOLOGIAS Portugalia Wkład UE Brak danych Adres Rua ALVES REDOL 9 LISBOA Zobacz na mapie Linki Strona internetowa Opens in new window Koszt całkowity Brak danych NATIONAL UNIVERSITY OF IRELAND, DUBLIN. UNIVERSITY COLLEGE DUBLIN Irlandia Wkład UE Brak danych Adres Belfield DUBLIN Zobacz na mapie Linki Strona internetowa Opens in new window Koszt całkowity Brak danych