Objetivo The requirement to screen for known SNPs (single nucleotide polymorphisms) has become one of the key challenges to be addressed to enable the exploitation of the human genome sequence, where approximately 3 million SNPs are responsible for all of the variation within the human population. Several methods and technologies are currently available for detection of SNPs, but no single platform exists which can deliver 100% accuracy, in a low cost, versatile, and easy-to-use integrated system.The proposed research will focus on the development of integrated SNP detection platforms to include modules for DNA extraction and purification from biological samples, DNA amplification, DNA characterisation (including SNP detection), signal transduction, interpretation and data analysis. Integration of the modules on a single platform will include a transport mechanism based on electrowetting actuation (EWOD).Two alternatives SNP detection platforms will be developed based on optical and magnetic sensing respectively. The whole system will be packaged as a single automated functional system with a simplified GUI interface, based on specifications recommended by potential end-users within the consortium. This systems development will focus on delivering a product customised for low to medium throughput, low cost, point-of-care applications, with emphasis on providing very rapid and accurate results.Within the project, the systems will be benchmarked for screening of SNPs in the CFTR gene that are associated with cystic fibrosis. CF patient samples will be used to verify the accuracy and reproducibility of the system in a clinical diagnostics laboratory. Ámbito científico engineering and technologyelectrical engineering, electronic engineering, information engineeringelectronic engineeringcontrol systemsengineering and technologyelectrical engineering, electronic engineering, information engineeringelectronic engineeringsensorsoptical sensorsmedical and health sciencesbasic medicinepharmacology and pharmacypharmaceutical drugsnatural sciencesbiological sciencesgeneticsDNAnatural sciencesbiological sciencesgeneticsnucleotides Palabras clave Nanotechnology Programa(s) FP6-NMP - Nanotechnologies and nanosciences, knowledge-based multifunctional materials and new production processes and devices: thematic priority 3 under the 'Focusing and integrating community research' of the 'Integrating and strengthening the European Research Area' specific programme 2002-2006. Tema(s) NMP-2003-3.4.1.5-2 - Ethical, legal, social aspects of research in nanotechnology NMP-2004-IST-NMP-2 - Bio-sensors for Diagnosis and Healthcare Convocatoria de propuestas FP6-2004-IST-NMP-2 Consulte otros proyectos de esta convocatoria Régimen de financiación STREP - Specific Targeted Research Project Coordinador UNIVERSITY COLLEGE CORK, NATIONAL UNIVERSITY OF IRELAND, CORK Aportación de la UE Sin datos Dirección Lee Maltings, CORK Irlanda Ver en el mapa Enlaces Sitio web Opens in new window Coste total Sin datos Participantes (6) Ordenar alfabéticamente Ordenar por aportación de la UE Ampliar todo Contraer todo ASPER BIOTECH LTD Estonia Aportación de la UE Sin datos Dirección Oru 3 TARTU Ver en el mapa Enlaces Sitio web Opens in new window Coste total Sin datos CHARLES UNIVERSITY PRAGUE Chequia Aportación de la UE Sin datos Dirección Ovocny TRH 5 PRAGUE 1 Ver en el mapa Enlaces Sitio web Opens in new window Coste total Sin datos COMMISSARIAT A L'ENERGIE ATOMIQUE (CEA) Francia Aportación de la UE Sin datos Dirección 31-33 rue de la Fédération PARIS Ver en el mapa Enlaces Sitio web Opens in new window Coste total Sin datos FUNDACAO DA FACULDADE DE CIENCIAS DA UNIVERSIDADE DE LISBOA Portugal Aportación de la UE Sin datos Dirección Campo Grande - Edificio C7 - 1 piso LISBOA Ver en el mapa Enlaces Sitio web Opens in new window Coste total Sin datos INSTITUTO DE ENGENHARIA DE SISTEMAS E COMPUTADORES PARA OS MICROSISTEMAS E AS NANOTECNOLOGIAS Portugal Aportación de la UE Sin datos Dirección Rua ALVES REDOL 9 LISBOA Ver en el mapa Enlaces Sitio web Opens in new window Coste total Sin datos NATIONAL UNIVERSITY OF IRELAND, DUBLIN. UNIVERSITY COLLEGE DUBLIN Irlanda Aportación de la UE Sin datos Dirección Belfield DUBLIN Ver en el mapa Enlaces Sitio web Opens in new window Coste total Sin datos