Obiettivo Neural stem cells (NSCs) have emerged as a major topic in neurobiology. The persistence of multipotent cells in the adult mammalian brain offers a realistic chance for the treatment of neurodegenerative diseases. It is thus crucial to understand NSCs from as many as possible angles (e. g. cellular and molecular biology), in order to better isolate and successfully manipulate them. CISSTEM presents a post-genomic systems biology approach, taking advantage of new computational and experimental tools to address the specification and maintenance of NSCs at the transcriptional/epigenetic level. CISSTEM is designed to unravel the basic principles of gene regulation in NSC, with a focus on cis-regulatory modules (CRMs). To do so we follow a multidisciplinary approach tightly interconnecting computational prediction and experimental validation in vitro and in vivo using different vertebrate models systems. Major intermediate objectives of this project are the prediction of relevant elements and the identification of the temporal, spatial and quantitative activities of predicted conserved regulatory motifs associated with NSC expressed genes. To achieve this goal, CISSTEM will i) Develop computational tools and resources for the in silico identification of CRMs and transcription factor binding sites (TFBSs) ii) Identify genomic regions functionally active in the NSC through direct mapping of DNase I hypersensitivity sites. Putative functional annotation to these sites will be implemented and conduct direct CRM prediction of the identified regions iii) Identify motifs/signatures over-represented in distal elements found around NSC expressed genes iv) Identify the elements acting as transcriptional enhancers and define their pattern of activities in different neural cell lines and in vertebrate animal model systems v) Disseminate the results of the project to the research community, biotech/pharmaceutical companies and the general public Campo scientifico natural sciencesbiological sciencesneurobiologymedical and health sciencesmedical biotechnologycells technologiesstem cellsnatural sciencesbiological sciencesmolecular biology Programma(i) FP7-HEALTH - Specific Programme "Cooperation": Health Argomento(i) HEALTH-2007-2.1.2-5 - Multidisciplinary fundamental genomics and molecular biology approaches to study basic biological processes relevant to health and diseases Invito a presentare proposte FP7-HEALTH-2007-B Vedi altri progetti per questo bando Meccanismo di finanziamento CP-FP - Small or medium-scale focused research project Coordinatore INSTITUT NATIONAL DE RECHERCHE POUR L'AGRICULTURE, L'ALIMENTATION ET L'ENVIRONNEMENT Contributo UE € 455 282,00 Indirizzo 147 RUE DE L'UNIVERSITE 75007 Paris Francia Mostra sulla mappa Regione Ile-de-France Ile-de-France Paris Tipo di attività Research Organisations Contatto amministrativo Jean-Stéphane Joly (Dr.) Collegamenti Contatta l’organizzazione Opens in new window Sito web Opens in new window Costo totale Nessun dato Partecipanti (8) Classifica in ordine alfabetico Classifica per Contributo UE Espandi tutto Riduci tutto KARLSRUHER INSTITUT FUER TECHNOLOGIE Germania Contributo UE € 441 749,00 Indirizzo KAISERSTRASSE 12 76131 Karlsruhe Mostra sulla mappa Regione Baden-Württemberg Karlsruhe Karlsruhe, Stadtkreis Tipo di attività Higher or Secondary Education Establishments Contatto amministrativo Natascha Wallburg (Ms.) Collegamenti Contatta l’organizzazione Opens in new window Sito web Opens in new window Costo totale Nessun dato UNIVERSITA' DEGLI STUDI DI MILANO-BICOCCA Italia Contributo UE € 353 360,00 Indirizzo PIAZZA DELL'ATENEO NUOVO 1 20126 MILANO Mostra sulla mappa Regione Nord-Ovest Lombardia Milano Tipo di attività Higher or Secondary Education Establishments Contatto amministrativo Anastasia Sguera (Dr.) Collegamenti Contatta l’organizzazione Opens in new window Sito web Opens in new window Costo totale Nessun dato EUROPEAN MOLECULAR BIOLOGY LABORATORY Germania Contributo UE € 672 800,00 Indirizzo Meyerhofstrasse 1 69117 Heidelberg Mostra sulla mappa Regione Baden-Württemberg Karlsruhe Heidelberg, Stadtkreis Tipo di attività Research Organisations Contatto amministrativo Phil Irving (Dr.) Collegamenti Contatta l’organizzazione Opens in new window Sito web Opens in new window Costo totale Nessun dato THE UNIVERSITY OF MANCHESTER Regno Unito Contributo UE € 186 644,00 Indirizzo OXFORD ROAD M13 9PL Manchester Mostra sulla mappa Regione North West (England) Greater Manchester Manchester Tipo di attività Higher or Secondary Education Establishments Contatto amministrativo Liz Fay (Ms.) Collegamenti Contatta l’organizzazione Opens in new window Sito web Opens in new window Costo totale Nessun dato RUPRECHT-KARLS-UNIVERSITAET HEIDELBERG Germania Contributo UE € 357 300,00 Indirizzo SEMINARSTRASSE 2 69117 Heidelberg Mostra sulla mappa Regione Baden-Württemberg Karlsruhe Heidelberg, Stadtkreis Tipo di attività Higher or Secondary Education Establishments Contatto amministrativo Laurence Ettwiller (Dr.) Collegamenti Contatta l’organizzazione Opens in new window Sito web Opens in new window Costo totale Nessun dato MEDICAL RESEARCH COUNCIL Regno Unito Contributo UE € 223 381,00 Indirizzo 20 Park Crescent W1B 1AL LONDON Mostra sulla mappa Tipo di attività Public bodies (excluding Research Organisations and Secondary or Higher Education Establishments) Contatto amministrativo Michele Marron (Ms.) Collegamenti Contatta l’organizzazione Opens in new window Sito web Opens in new window Costo totale Nessun dato CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS Francia Contributo UE € 175 560,00 Indirizzo RUE MICHEL ANGE 3 75794 Paris Mostra sulla mappa Regione Ile-de-France Ile-de-France Paris Tipo di attività Research Organisations Contatto amministrativo Michele Saumon (Ms.) Collegamenti Contatta l’organizzazione Opens in new window Sito web Opens in new window Costo totale Nessun dato INRAE TRANSFERT SAS Francia Contributo UE € 118 093,00 Indirizzo RUE DU DOCTEUR FINLAY 28 75015 Paris Mostra sulla mappa Regione Ile-de-France Ile-de-France Paris Tipo di attività Private for-profit entities (excluding Higher or Secondary Education Establishments) Contatto amministrativo Lucie Hofmann (Ms.) Collegamenti Contatta l’organizzazione Opens in new window Sito web Opens in new window Costo totale Nessun dato