Objetivo A novel analytical platform is proposed to detect and identify DNA at low concentration in a high throughput manner at the single molecule level.The potential impact of this research is significant and will result in single molecule detection becoming a mainstream tool within the medical diagnostics and analytical communities. 'Rare event' detection plays an important role in the early detection of illnesses and disease (e.g. cancers and bacterial infections). Using analytical technologies that exist today it is almost impossible to detect a single DNA strand within a standard blood sample (of a few mLs) within a reasonable time frame. The technology that will be developed within the current project will allow for such detection to be performed both rapidly and efficiently. If successful, the core technology described will become a mainstream analytical tool that will be of significant benefit within biomedical laboratories, hospitals, and clinics around the world.Specifically, chemical and semiconductor processing methods will be developed to define a novel approach to high throughput DNA quantification at the single molecule level. This innovative technology will function by introducing biological samples in micro- and nanofluidic chips and using electric fields to direct DNA strands through nanometre-sized pores on a membrane. Detection and sizing of the individual DNA strands (labelled with fluorophores) is then accomplished using confocal fluorescence spectroscopy.This new approach to high-throughput, single molecule DNA analysis harnesses the strengths of both analytical spectroscopy and silicon fabrication technology to allow the creation of hybrid devices in which molecular quantification can be realized. I expect this work to have major impact and open up new possibilities for nano-analytical tools in the chemical and biological sciences. Ámbito científico natural sciencesbiological sciencesgeneticsDNAnatural sciencesphysical sciencesopticsspectroscopyemission spectroscopymedical and health sciencesclinical medicineoncologynatural sciencesphysical scienceselectromagnetism and electronicssemiconductivitynatural scienceschemical sciencesinorganic chemistrymetalloids Programa(s) FP7-IDEAS-ERC - Specific programme: "Ideas" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Tema(s) ERC-SG-PE4 - ERC Starting Grant - Physical and Analytical Chemical sciences Convocatoria de propuestas ERC-2011-StG_20101014 Consulte otros proyectos de esta convocatoria Régimen de financiación ERC-SG - ERC Starting Grant Institución de acogida IMPERIAL COLLEGE OF SCIENCE TECHNOLOGY AND MEDICINE Aportación de la UE € 1 497 620,00 Dirección SOUTH KENSINGTON CAMPUS EXHIBITION ROAD SW7 2AZ LONDON Reino Unido Ver en el mapa Región London Inner London — West Westminster Tipo de actividad Higher or Secondary Education Establishments Contacto administrativo Brooke Alasya (Ms.) Investigador principal Joshua Edel (Dr.) Enlaces Contactar con la organización Opens in new window Sitio web Opens in new window Coste total Sin datos Beneficiarios (1) Ordenar alfabéticamente Ordenar por aportación de la UE Ampliar todo Contraer todo IMPERIAL COLLEGE OF SCIENCE TECHNOLOGY AND MEDICINE Reino Unido Aportación de la UE € 1 497 620,00 Dirección SOUTH KENSINGTON CAMPUS EXHIBITION ROAD SW7 2AZ LONDON Ver en el mapa Región London Inner London — West Westminster Tipo de actividad Higher or Secondary Education Establishments Contacto administrativo Brooke Alasya (Ms.) Investigador principal Joshua Edel (Dr.) Enlaces Contactar con la organización Opens in new window Sitio web Opens in new window Coste total Sin datos