Objective A pivotal question in developmental biology is how cellular gene regulatory networks can respond to a signalling molecule in a concentration-dependent manner. This can now be studied using the modern tools of synthetic biology, an emerging research field that applies engineering approaches to biological systems.This project aims to adapt and develop a band-forming model in E. coli. It will explore systematically the parameter and network space that regulates the formation of a single stripe of gene expression in a morphogen gradient. The full design space of 3-gene networks, where one gene is activated by the morphogen, is 9,710 (isometric networks removed). We are therefore building a flexible network 'scaffold' that will allow the construction of any such circuit and, in particular, the six core topologies predicted in silico to form single stripes.Rational design of gene regulatory networks in vivo is extremely challenging, due to the complex interactions in living cells. Therefore, we are exploring the parameter space that leads to functional band-forming networks by building combinatorial libraries, followed by selection for an appropriate survival pressure.The synthetic networks are engineered with the potential to react as concentration band filters, with respect to a morphogen concentration. The RNA polymerases from the T7 and SP6 phages are used as activators and artificial zinc finger DNA-binding domains, cI and lacI as repressors. The output of the gene regulatory networks is a selectable gene, fused to a green fluorescent protein, which can be used for selection, counterselection and quantification. Fields of science natural sciencesbiological sciencessynthetic biologynatural sciencesbiological sciencesmicrobiologyvirologynatural scienceschemical sciencesinorganic chemistrytransition metalsnatural sciencesmathematicspure mathematicstopologynatural sciencesbiological sciencesdevelopmental biology Programme(s) FP7-PEOPLE - Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Topic(s) FP7-PEOPLE-2011-IEF - Marie-Curie Action: "Intra-European fellowships for career development" Call for proposal FP7-PEOPLE-2011-IEF See other projects for this call Funding Scheme MC-IEF - Intra-European Fellowships (IEF) Coordinator FUNDACIO CENTRE DE REGULACIO GENOMICA EU contribution € 168 896,40 Address CARRER DOCTOR AIGUADER 88 08003 Barcelona Spain See on map Region Este Cataluña Barcelona Activity type Research Organisations Administrative Contact Stefan Ponisch (Mr.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data