Objective
A pivotal question in developmental biology is how cellular gene regulatory networks can respond to a signalling molecule in a concentration-dependent manner. This can now be studied using the modern tools of synthetic biology, an emerging research field that applies engineering approaches to biological systems.
This project aims to adapt and develop a band-forming model in E. coli. It will explore systematically the parameter and network space that regulates the formation of a single stripe of gene expression in a morphogen gradient. The full design space of 3-gene networks, where one gene is activated by the morphogen, is 9,710 (isometric networks removed). We are therefore building a flexible network 'scaffold' that will allow the construction of any such circuit and, in particular, the six core topologies predicted in silico to form single stripes.
Rational design of gene regulatory networks in vivo is extremely challenging, due to the complex interactions in living cells. Therefore, we are exploring the parameter space that leads to functional band-forming networks by building combinatorial libraries, followed by selection for an appropriate survival pressure.
The synthetic networks are engineered with the potential to react as concentration band filters, with respect to a morphogen concentration. The RNA polymerases from the T7 and SP6 phages are used as activators and artificial zinc finger DNA-binding domains, cI and lacI as repressors. The output of the gene regulatory networks is a selectable gene, fused to a green fluorescent protein, which can be used for selection, counterselection and quantification.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences synthetic biology
- natural sciences biological sciences microbiology virology
- natural sciences chemical sciences inorganic chemistry transition metals
- natural sciences mathematics pure mathematics topology
- natural sciences biological sciences developmental biology
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-2011-IEF
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
08003 Barcelona
Spain
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.