cerebellar molecular layer Interneuron network imaging in awake animal

Projektinformationen

Interneuron Network

ID Finanzhilfevereinbarung: 302819

Abgeschlossenes Projekt

Startdatum 1 April 2012

Enddatum 31 März 2014

Finanziert unter

• Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)

Gesamtkosten € 201 932,40

EU-Beitrag € 201 932,40

Koordiniert durch

CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS
France

Ziel

Molecular layer interneurons form an interconnected network in the cerebellar cortex that controls the activity of Purkinje cells. Even though much is known about the properties of these interneurons in isolated preparations, their activity pattern in vivo remains largely unknown. The present project proposes to investigate the functional status of the interneurons in awake, head-restrained mice, by performing two photon calcium imaging of the cerebellum with genetically-encoded calcium indicator GCaMP3. Imaging speed will be improved by implementing random access multi point scanning with acousto-optic deflectors. Once the basic activity pattern will be known, effect of sensory stimulation (tactile stimulation or eye puff) and rhythmic behavior (licking behavior) will be explored. In a second step of the project, calcium imaging of the interneurons will be combined with whole-cell recordings of postsynaptic Purkinje cells. In this manner the effects of interneuron network activity on the overall cerebellar circuit will be assessed. The results will be evaluated with the help of a collaborating modeling team. This study will shed light on the functional role of molecular layer interneurons, and more generally, on the way by which interneurons regulate the activity of brain network. It will also provide data that will help evaluating consequences of defects of gabaergic transmission in cerebellar pathologies. The applicant will benefit from the multidisciplinary training provided by this project.

Wissenschaftliches Gebiet

• natural sciences › chemical sciences › inorganic chemistry › alkaline earth metals
• medical and health sciences › basic medicine › pathology
• natural sciences › physical sciences › theoretical physics › particle physics › photons

Programm/Programme

• FP7-PEOPLE - Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)

Thema/Themen

• FP7-PEOPLE-2011-IEF - Marie-Curie Action: "Intra-European fellowships for career development"

Aufforderung zur Vorschlagseinreichung

FP7-PEOPLE-2011-IEF
Andere Projekte für diesen Aufruf anzeigen

Finanzierungsplan

MC-IEF - Intra-European Fellowships (IEF)

Koordinator

CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS

Kontakt Verwaltung

Franck Charron (Mr.)

• EU-Beitrag: € 201 932,40
• Adresse: RUE MICHEL ANGE 3 ; 75794 Paris ; Frankreich
• Region: Ile-de-France › Ile-de-France › Paris
• Aktivitätstyp: Research Organisations
• Gesamtkosten: Keine Daten