Obiettivo Interactions between neurons and glial cells underlie the normal development, structure and function of the nervous system. Perturbation of this communication occurs during injury, in acquired or hereditary neuropathic diseases of the central and peripheral nervous systems, and with ageing. However, the specific nature and the physiological significance of neuron-glia interactions remain largely unknown. We propose here the use of a microfluidic co-culture platform to characterize and investigate the role of non-synaptic responses of glial cells following stimulation of neuronal activity. In particular, we will co-culture neurons and glial cells of the peripheral nervous system, and monitor glia-specific changes at the transcriptome- and the secretome-levels, after manipulation of the electrical activity of neurons. We will subsequently interfere with the identified signaling pathways, and study effects on neuronal structure and function. Following this in vitro approach, we expect to identify potential mediators of the axon-glia communication and decipher their role in maintaining the nervous system integrity and function. Campo scientifico natural sciencesbiological sciencesneurobiologynatural sciencesphysical sciencesclassical mechanicsfluid mechanicsmicrofluidics Programma(i) FP7-PEOPLE - Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Argomento(i) FP7-PEOPLE-2011-IIF - Marie Curie Action: "International Incoming Fellowships" Invito a presentare proposte FP7-PEOPLE-2011-IIF Vedi altri progetti per questo bando Meccanismo di finanziamento MC-IIF - International Incoming Fellowships (IIF) Coordinatore UNIVERSITE DE LAUSANNE Contributo UE € 184 709,40 Indirizzo QUARTIER UNIL CENTRE - BATIMENT UNICENTRE 1015 LAUSANNE Svizzera Mostra sulla mappa Regione Schweiz/Suisse/Svizzera Région lémanique Vaud Tipo di attività Higher or Secondary Education Establishments Contatto amministrativo Roman Chrast (Prof.) Collegamenti Contatta l’organizzazione Opens in new window Sito web Opens in new window Costo totale Nessun dato