Objetivo Fluorescence techniques provide powerful means to study single protein machineries. Single molecule observation of Fluorescence Resonance Energy Transfer (FRET) has become an important tool for probing structure, distances, dynamics, physicochemical properties and size of purified (in vitro) protein complexes. Super-resolution techniques provide complementary spatial information about proteins in live (in vivo) specimen. Although these techniques have become very popular because of their high sensitivity, their throughput is limited by large statistical sampling requirements, complex sample preparation procedures and/or laborious data acquisition workflows. Here, I propose to design a unified single molecule approach by engineering ultrafast, semi-synthetic protein modification techniques and fluorescent readouts into nano/microfluidic hybrid devices. The development of an automated all-in-one Lab-on-a-Chip platform will dramatically improve throughput of single molecule fluorescence techniques. The technology will provide a multiparameter readout of molecular protein mechanisms across time, resolution and complexity scales in a generalised workflow. To demonstrate the power of the new platform, I will apply it to a mechanistic study of the multifunctionality of intrinsically disordered proteins (IDPs), which are vital to cellular function and associated with many disease mechanisms. The polymeric properties of IDPs enable them to populate a variety of states and engage with various cellular binding partners, thus exceeding the sampling limit of existing single molecule technologies. The new method will blur the boundaries between in cell superresolution microscopy and biochemical single molecule studies and unleash the true power of single molecule protein science for a range of applications in analytical sciences, drug discovery, biotechnology, systems biology and fundamental biomedical research. Ámbito científico medical and health sciencesbasic medicinepharmacology and pharmacydrug discoverynatural sciencesphysical sciencesopticsmicroscopysuper resolution microscopynatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsnatural scienceschemical sciencesorganic chemistryaminesnatural sciencesphysical sciencesopticsspectroscopy Programa(s) H2020-EU.1.1. - EXCELLENT SCIENCE - European Research Council (ERC) Main Programme Tema(s) ERC-CoG-2014 - ERC Consolidator Grant Convocatoria de propuestas ERC-2014-CoG Consulte otros proyectos de esta convocatoria Régimen de financiación ERC-COG - Consolidator Grant Institución de acogida JOHANNES GUTENBERG-UNIVERSITAT MAINZ Aportación neta de la UEn € 542 673,46 Dirección SAARSTRASSE 21 55122 Mainz Alemania Ver en el mapa Región Rheinland-Pfalz Rheinhessen-Pfalz Mainz, Kreisfreie Stadt Tipo de actividad Higher or Secondary Education Establishments Enlaces Contactar con la organización Opens in new window Sitio web Opens in new window Participación en los programas de I+D de la UE Opens in new window Red de colaboración de HORIZON Opens in new window Coste total € 542 673,46 Beneficiarios (2) Ordenar alfabéticamente Ordenar por aportación neta de la UE Ampliar todo Contraer todo JOHANNES GUTENBERG-UNIVERSITAT MAINZ Alemania Aportación neta de la UEn € 542 673,46 Dirección SAARSTRASSE 21 55122 Mainz Ver en el mapa Región Rheinland-Pfalz Rheinhessen-Pfalz Mainz, Kreisfreie Stadt Tipo de actividad Higher or Secondary Education Establishments Enlaces Contactar con la organización Opens in new window Sitio web Opens in new window Participación en los programas de I+D de la UE Opens in new window Red de colaboración de HORIZON Opens in new window Coste total € 542 673,46 EUROPEAN MOLECULAR BIOLOGY LABORATORY Alemania Aportación neta de la UEn € 1 454 316,54 Dirección Meyerhofstrasse 1 69117 Heidelberg Ver en el mapa Región Baden-Württemberg Karlsruhe Heidelberg, Stadtkreis Tipo de actividad Research Organisations Enlaces Contactar con la organización Opens in new window Sitio web Opens in new window Participación en los programas de I+D de la UE Opens in new window Red de colaboración de HORIZON Opens in new window Coste total € 1 454 316,54