Reconstitution of Chromosome Replication and Epigenetic Inheritance

Project Information

CHROMOREP

Grant agreement ID: 669424

DOI 10.3030/669424

Project closed

EC signature date 28 July 2015

Start date 1 November 2015

End date 30 April 2022

Funded under

• EXCELLENT SCIENCE - European Research Council (ERC)

Total cost € 1 983 019,00

EU contribution € 1 983 019,00

Coordinated by

THE FRANCIS CRICK INSTITUTE LIMITED
United Kingdom

Publications

Peer reviewed articles (13)

Rad53 checkpoint kinase regulation of DNA replication fork rate via Mrc1 phosphorylation

Author(s): Allison W McClure; John FX Diffley
Published in: eLife, Issue 2, 2022, ISSN 2050-084X
Publisher: eLife Sciences Publications
DOI: 10.1101/2021.04.09.439171

The mechanism of eukaryotic CMG helicase activation

Author(s): Max E. Douglas, Ferdos Abid Ali, Alessandro Costa, John F. X. Diffley
Published in: Nature, Issue 555/7695, 2018, Page(s) 265-268, ISSN 0028-0836
Publisher: Nature Publishing Group
DOI: 10.1038/nature25787

Bidirectional eukaryotic DNA replication is established by quasi-symmetrical helicase loading

Author(s): Gideon Coster, John F. X. Diffley
Published in: Science, Issue 357/6348, 2017, Page(s) 314-318, ISSN 0036-8075
Publisher: American Association for the Advancement of Science
DOI: 10.1126/science.aan0063

Cryo-EM structure of a licensed DNA replication origin

Author(s): Ferdos Abid Ali, Max E. Douglas, Julia Locke, Valerie E. Pye, Andrea Nans, John F. X. Diffley, Alessandro Costa
Published in: Nature Communications, Issue 8/1, 2017, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-017-02389-0

How the Eukaryotic Replisome Achieves Rapid and Efficient DNA Replication

Author(s): Joseph T.P. Yeeles, Agnieska Janska, Anne Early, John F.X. Diffley
Published in: Molecular Cell, Issue 65/1, 2017, Page(s) 105-116, ISSN 1097-2765
Publisher: Cell Press
DOI: 10.1016/j.molcel.2016.11.017

Chromatin Controls DNA Replication Origin Selection, Lagging-Strand Synthesis, and Replication Fork Rates

Author(s): Christoph F. Kurat, Joseph T.P. Yeeles, Harshil Patel, Anne Early, John F.X. Diffley
Published in: Molecular Cell, Issue 65/1, 2017, Page(s) 117-130, ISSN 1097-2765
Publisher: Cell Press
DOI: 10.1016/j.molcel.2016.11.016

Cdt1 stabilizes an open MCM ring for helicase loading

Author(s): Jordi Frigola, Jun He, Kerstin Kinkelin, Valerie E. Pye, Ludovic Renault, Max E. Douglas, Dirk Remus, Peter Cherepanov, Alessandro Costa, John F. X. Diffley
Published in: Nature Communications, Issue 8, 2017, Page(s) 15720, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/ncomms15720

CMG–Pol epsilon dynamics suggests a mechanism for the establishment of leading-strand synthesis in the eukaryotic replisome

Author(s): Jin Chuan Zhou, Agnieszka Janska, Panchali Goswami, Ludovic Renault, Ferdos Abid Ali, Abhay Kotecha, John F. X. Diffley, Alessandro Costa
Published in: Proceedings of the National Academy of Sciences, Issue 114/16, 2017, Page(s) 4141-4146, ISSN 0027-8424
Publisher: National Academy of Sciences
DOI: 10.1073/pnas.1700530114

Rpd3L Contributes to the DNA Damage Sensitivity of Saccharomyces cerevisiae Checkpoint Mutants

Author(s): Belén Gómez-González, Harshil Patel, Anne Early, John F. X. Diffley
Published in: Genetics, Issue 211/2, 2019, Page(s) 503-513, ISSN 0016-6731
Publisher: Genetics Society of America
DOI: 10.1534/genetics.118.301817

Mechanism of head-to-head MCM double-hexamer formation revealed by cryo-EM

Author(s): Thomas C. R. Miller, Julia Locke, Julia F. Greiwe, John F. X. Diffley, Alessandro Costa
Published in: Nature, Issue 575/7784, 2019, Page(s) 704-710, ISSN 0028-0836
Publisher: Nature Publishing Group
DOI: 10.1038/s41586-019-1768-0

Structure of DNA-CMG-Pol epsilon elucidates the roles of the non-catalytic polymerase modules in the eukaryotic replisome

Author(s): Panchali Goswami, Ferdos Abid Ali, Max E. Douglas, Julia Locke, Andrew Purkiss, Agnieszka Janska, Patrik Eickhoff, Anne Early, Andrea Nans, Alan M. C. Cheung, John F. X. Diffley, Alessandro Costa
Published in: Nature Communications, Issue 9/1, 2018, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-018-07417-1

A DNA replication fork-centric view of the budding yeast DNA damage response

Author(s): Allison W. McClure; Berta Canal; John F.X. Diffley
Published in: eLife, Issue 2, 2022, ISSN 2050-084X
Publisher: eLife Sciences Publications
DOI: 10.25418/crick.21164149

Budding yeast Rap1, but not telomeric DNA, is inhibitory for multiple stages of DNA replication in vitro.

Author(s): Max E. Douglas; John F.X. Diffley
Published in: Nucleic Acids Research, Issue 1, 2021, ISSN 0305-1048
Publisher: Oxford University Press
DOI: 10.1093/nar/gkab416