Reconstitution of Chromosome Replication and Epigenetic Inheritance

Información del proyecto

CHROMOREP

Identificador del acuerdo de subvención: 669424

DOI 10.3030/669424

Proyecto cerrado

Fecha de la firma de la CE 28 Julio 2015

Fecha de inicio 1 Noviembre 2015

Fecha de finalización 30 Abril 2022

Financiado con arreglo a

• EXCELLENT SCIENCE - European Research Council (ERC)

Coste total € 1 983 019,00

Aportación de la UE € 1 983 019,00

Coordinado por

THE FRANCIS CRICK INSTITUTE LIMITED
United Kingdom

Publicaciones

Peer reviewed articles (13)

Rad53 checkpoint kinase regulation of DNA replication fork rate via Mrc1 phosphorylation

Autores: Allison W McClure; John FX Diffley
Publicado en: eLife, Edición 2, 2022, ISSN 2050-084X
Editor: eLife Sciences Publications
DOI: 10.1101/2021.04.09.439171

The mechanism of eukaryotic CMG helicase activation

Autores: Max E. Douglas, Ferdos Abid Ali, Alessandro Costa, John F. X. Diffley
Publicado en: Nature, Edición 555/7695, 2018, Página(s) 265-268, ISSN 0028-0836
Editor: Nature Publishing Group
DOI: 10.1038/nature25787

Bidirectional eukaryotic DNA replication is established by quasi-symmetrical helicase loading

Autores: Gideon Coster, John F. X. Diffley
Publicado en: Science, Edición 357/6348, 2017, Página(s) 314-318, ISSN 0036-8075
Editor: American Association for the Advancement of Science
DOI: 10.1126/science.aan0063

Cryo-EM structure of a licensed DNA replication origin

Autores: Ferdos Abid Ali, Max E. Douglas, Julia Locke, Valerie E. Pye, Andrea Nans, John F. X. Diffley, Alessandro Costa
Publicado en: Nature Communications, Edición 8/1, 2017, ISSN 2041-1723
Editor: Nature Publishing Group
DOI: 10.1038/s41467-017-02389-0

How the Eukaryotic Replisome Achieves Rapid and Efficient DNA Replication

Autores: Joseph T.P. Yeeles, Agnieska Janska, Anne Early, John F.X. Diffley
Publicado en: Molecular Cell, Edición 65/1, 2017, Página(s) 105-116, ISSN 1097-2765
Editor: Cell Press
DOI: 10.1016/j.molcel.2016.11.017

Chromatin Controls DNA Replication Origin Selection, Lagging-Strand Synthesis, and Replication Fork Rates

Autores: Christoph F. Kurat, Joseph T.P. Yeeles, Harshil Patel, Anne Early, John F.X. Diffley
Publicado en: Molecular Cell, Edición 65/1, 2017, Página(s) 117-130, ISSN 1097-2765
Editor: Cell Press
DOI: 10.1016/j.molcel.2016.11.016

Cdt1 stabilizes an open MCM ring for helicase loading

Autores: Jordi Frigola, Jun He, Kerstin Kinkelin, Valerie E. Pye, Ludovic Renault, Max E. Douglas, Dirk Remus, Peter Cherepanov, Alessandro Costa, John F. X. Diffley
Publicado en: Nature Communications, Edición 8, 2017, Página(s) 15720, ISSN 2041-1723
Editor: Nature Publishing Group
DOI: 10.1038/ncomms15720

CMG–Pol epsilon dynamics suggests a mechanism for the establishment of leading-strand synthesis in the eukaryotic replisome

Autores: Jin Chuan Zhou, Agnieszka Janska, Panchali Goswami, Ludovic Renault, Ferdos Abid Ali, Abhay Kotecha, John F. X. Diffley, Alessandro Costa
Publicado en: Proceedings of the National Academy of Sciences, Edición 114/16, 2017, Página(s) 4141-4146, ISSN 0027-8424
Editor: National Academy of Sciences
DOI: 10.1073/pnas.1700530114

Rpd3L Contributes to the DNA Damage Sensitivity of Saccharomyces cerevisiae Checkpoint Mutants

Autores: Belén Gómez-González, Harshil Patel, Anne Early, John F. X. Diffley
Publicado en: Genetics, Edición 211/2, 2019, Página(s) 503-513, ISSN 0016-6731
Editor: Genetics Society of America
DOI: 10.1534/genetics.118.301817

Mechanism of head-to-head MCM double-hexamer formation revealed by cryo-EM

Autores: Thomas C. R. Miller, Julia Locke, Julia F. Greiwe, John F. X. Diffley, Alessandro Costa
Publicado en: Nature, Edición 575/7784, 2019, Página(s) 704-710, ISSN 0028-0836
Editor: Nature Publishing Group
DOI: 10.1038/s41586-019-1768-0

Structure of DNA-CMG-Pol epsilon elucidates the roles of the non-catalytic polymerase modules in the eukaryotic replisome

Autores: Panchali Goswami, Ferdos Abid Ali, Max E. Douglas, Julia Locke, Andrew Purkiss, Agnieszka Janska, Patrik Eickhoff, Anne Early, Andrea Nans, Alan M. C. Cheung, John F. X. Diffley, Alessandro Costa
Publicado en: Nature Communications, Edición 9/1, 2018, ISSN 2041-1723
Editor: Nature Publishing Group
DOI: 10.1038/s41467-018-07417-1

A DNA replication fork-centric view of the budding yeast DNA damage response

Autores: Allison W. McClure; Berta Canal; John F.X. Diffley
Publicado en: eLife, Edición 2, 2022, ISSN 2050-084X
Editor: eLife Sciences Publications
DOI: 10.25418/crick.21164149

Budding yeast Rap1, but not telomeric DNA, is inhibitory for multiple stages of DNA replication in vitro.

Autores: Max E. Douglas; John F.X. Diffley
Publicado en: Nucleic Acids Research, Edición 1, 2021, ISSN 0305-1048
Editor: Oxford University Press
DOI: 10.1093/nar/gkab416