Descripción del proyecto DEENESFRITPL Aprovechar la luz para el control celular La optogenética es una tecnología revolucionaria que permite activar y controlar con precisión las funciones biológicas de las neuronas u otros tipos de células diseñadas para responder a la luz. Este método experimental introduce en las células proteínas fotoactivadas y facilita la caracterización y manipulación precisas de las funciones celulares. Sin embargo, la capacidad de desactivar las células utilizando intensidades de luz moderadas o bajas sigue siendo un reto. El equipo del proyecto MERA, financiado por el Consejo Europeo de Investigación, analizará rodopsina-guanilato-ciclasa (RhGC), un nuevo fotorreceptor sensorial hallado en el hongo «Blastocladiella emersonii». El objetivo de los investigadores es comprender a fondo RhGC y liberar todo su potencial, lo que allanará el camino para diversas aplicaciones en optogenética y otros campos de investigación. Mostrar el objetivo del proyecto Ocultar el objetivo del proyecto Objetivo "Channelrhodopsin, which was discovered and described as a light-gated ion channel in my laboratory, has revolutionized the field of neuroscience over the past decade by enabling researchers to specifically activate selected neurons in a large ensemble of neuronal cells with short light flashes, a technology we now call ""Optogenetics."" However, though highly desirable, the inactivation of specific cells using moderate or low light intensities is not yet possible. The recently discovered rhodopsin-guanylyl-cyclase (RhGC) of the fungus Blastocladiella emersonii offers an elegant solution to this problem. Moreover, RhGC is a totally novel and uncharacterized sensory photoreceptor, and the first member of an enzyme rhodopsin family that urgently awaits in-depth characterization. Accordingly, the goal of the “mechanism of enzyme rhodopsin activation” (MERA) proposal is to obtain a comprehensive understanding of this novel photoreceptor, and to determine its functionality for broad application in optogenetics and other research fields. The MERA project is subdivided into four objectives. The first objective is the characterization and engineering of RhGC in cell lines and neurons as well as coexpression of RhGC with a cGMP-gated K+ channel to develop a ""Light-Hypopolarizer"" for cell inactivation. The second objective is to understand the dynamics of RhGC using a variety of biophysical technologies including time resolved UV-vis, FTIR, and Raman and EPR spectroscopy. A third objective is the generation of crystals for X-ray crystallography and the development of a three dimensional RhGC model. The fourth and final objective is the computer-aided conversion of RhGC into a rhodopsin-phosphodiesterase (RhPDE) for down-regulation of the second messenger cGMP and/or cAMP using light. The ultimate outcome will be a detailed understanding of a novel class of sensory photoreceptors with new perspectives for broad optogenetic applications." Ámbito científico natural sciencesbiological sciencesneurobiologynatural sciencesearth and related environmental sciencesgeologymineralogycrystallographynatural sciencesbiological sciencesgeneticsnucleotidesnatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsenzymesnatural sciencesphysical sciencesopticsspectroscopy Programa(s) H2020-EU.1.1. - EXCELLENT SCIENCE - European Research Council (ERC) Main Programme Tema(s) ERC-ADG-2015 - ERC Advanced Grant Convocatoria de propuestas ERC-2015-AdG Consulte otros proyectos de esta convocatoria Régimen de financiación ERC-ADG - Advanced Grant Institución de acogida HUMBOLDT-UNIVERSITAET ZU BERLIN Aportación neta de la UEn € 2 398 750,00 Dirección UNTER DEN LINDEN 6 10117 Berlin Alemania Ver en el mapa Región Berlin Berlin Berlin Tipo de actividad Higher or Secondary Education Establishments Enlaces Contactar con la organización Opens in new window Sitio web Opens in new window Participación en los programas de I+D de la UE Opens in new window Red de colaboración de HORIZON Opens in new window Coste total € 2 398 750,00 Beneficiarios (1) Ordenar alfabéticamente Ordenar por aportación neta de la UE Ampliar todo Contraer todo HUMBOLDT-UNIVERSITAET ZU BERLIN Alemania Aportación neta de la UEn € 2 398 750,00 Dirección UNTER DEN LINDEN 6 10117 Berlin Ver en el mapa Región Berlin Berlin Berlin Tipo de actividad Higher or Secondary Education Establishments Enlaces Contactar con la organización Opens in new window Sitio web Opens in new window Participación en los programas de I+D de la UE Opens in new window Red de colaboración de HORIZON Opens in new window Coste total € 2 398 750,00
