Multidisciplinary European training network for development of personalized anti-infective medical devices combining printing technologies and antimicrobial functionality

Project Information

PRINT-AID

Grant agreement ID: 722467

DOI 10.3030/722467

Project closed

EC signature date 9 August 2016

Start date 1 January 2017

End date 31 December 2020

Funded under

• EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions

Total cost € 2 265 900,12

EU contribution € 2 265 900,12

Coordinated by

HELSINGIN YLIOPISTO
Finland

Deliverables

Documents, reports (2)

A manual of standardized methods and minimum information guidelines for biofilm experiments

A manual containing the standardized methods and minimum information guidelines, for anti-biofilm efficacy endpoint methodologies relevant to the project.

Publications

PRINT-AID publications.

Websites, patent fillings, videos etc. (2)

PRINT-AID website and social media

Preparation of PRINT-AID website and social media outlets.

PRINT-AID online courses

Completed online courses for ESRs in PRINT-AID (and other students).

Other (4)

Methodological pipeline

A methodological pipeline/platform of methods and tools specifically optimized for chemical screens.

ESR recruitment course for ESR selection

Recruitment course for ESR organized in Porto. Co-lead beneficiary: UPORTO.

PRINT-AID Outreach Conference

Outreach conference organized.

ESRs local school visits

Visits to local schools by the ESRs to disseminate the project.

Publications

Conference proceedings (22)

Production and Characterization of Catheters Capable of Resisting Device Associated Infection

Author(s): Bahaa Shaqour, Clara Guarch Pérez, Augusto Vazquez-Rodriguez, Juliana Aizawa, Emilia Choińska, Martijn Riool, Bart Verleije, Koen Beyers, Livia Ferrari, Antonio Felici, Sebastian A. J. Zaat, Wojciech Święszkowski, Paul Cos
Published in: Abstract, Oral Presentation/Print-Aid Outreach Conference, 2020
Publisher: Print-Aid

Is gelatin metacrylate the future 3D printing antimicrobial coating?

Author(s): CM Guarch Pérez, D Martínez-Pérez, W Swieszkowski, M Riool, SAJ Zaat
Published in: Abstract, Oral Presentation/eCM XIX: Orthopaedic Infection, Davos, Switzerland, 2019, Page(s) 18
Publisher: eCM

Drop on demand antimicrobial coatings to combat biomaterial-associated infections

Author(s): D. Martínez Pérez, C. Guarch Pérez, I. Reigada, A. Fallarero, E. Choińska, M. Riool, S.A.J. Zaat, W. Święszkowski
Published in: Abstract, Oral Presentation/Print-Aid Outreach Conference, 2020
Publisher: Print-Aid

Evaluating biofilm experimental methods

Author(s): J. Allkja, A.E. Parker, NF. Azevedo, DM. Goeres
Published in: Abstract, Oral Presentation/Print-Aid Outreach Conference, 2020
Publisher: Print-Aid

Production of ciprofloxacin loaded tubes as positive control for evaluating Ventilator Associated Pneumonia prevention in a mouse model

Author(s): Juliana Aizawa, Bahaa Shaqour, Clara Guarch-Pérez, Emilia Choińska, Martijn Riool, Bart Verleije, Koen Beyers, Sebastian A. J. Zaat, Wojciech Święszkowski, Paul Cos
Published in: Abstract, Oral Presentation/Print-Aid Outreach Conference, 2020
Publisher: Print-Aid

Local delivery of the antimicrobial peptide SAAP-148 from a 3D-printed degradable PLGA coating to prevent orthopaedic infections

Author(s): C.M. Guarch Pérez, D. Martínez Pérez, M. Riool, W. Święszkowski, S.A.J. Zaat
Published in: Abstract, Oral Presentation/Print-Aid Outreach Conference, 2020
Publisher: Print-Aid

Applying drug repurposing to produce novel medical devices with antibacterial properties: In vitro and In vivo proof of concept

Author(s): Augusto Vazquez, Bahaa Shaqour, Bart Verleije, Koen Beyers, David Martinez, Wojciech Święszkowski, Clara Guarch Pérez, Sebastian Zaat, Livia Ferrari, Antonio Felici
Published in: Abstract, Oral Presentation/Print-Aid Outreach Conference, 2020
Publisher: Print-Aid

Optimization of a high-throughput screening platform in 384-well plates for exploration of anti-biofilm activity

Author(s): Shella Gilbert-Girard, Malena Skogman, Kirsi Savijoki, Jari Yli-Kauhaluoma and Adyary Fallarero
Published in: Abstracts, poster, Biofilms 8 Conference, 2018
Publisher: Biofilms 8 Conference

Effects of African marigold (Tagete erecta) on Staphylococcus aureus (ATCC25923), Staphylococcus aureus Newman and Staphylococcus epidermis (ATCC RP62A) biofilms

Author(s): Cristina Moliner; Inés Reigada; Carlota Gómez-Rincón; Malena Skogman; Adyary Fallarero
Published in: Abstract, poster, Biofilms 8 Conference, 2018
Publisher: Biofilms 8

Antimicrobial evaluation of a 3D-printed dual antibiotic-loaded PLGA microparticles-GelMa hydrogel

Author(s): Clara Mar Guarch Pérez, David Martínez Pérez, Martijn Riool, Wojciech Święszkowski, Sebastian Zaat
Published in: Abstract, Poster/Nederlandse Vereniging voor Medische Microbiologie (NVMM), 2019
Publisher: Nederlandse Vereniging voor Medische Microbiologie (NVMM)

Principles of method standardization

Author(s): Allkja, J., Parker, A.E., Azevedo, N.F., Goeres, D.M.
Published in: Abstract, Oral Presentation/eCM XIX: Orthopaedic Infection, Davos, Switzerland, 2019, 2019, Page(s) 17
Publisher: Orthopaedic Infection, Davos, Switzerland

Characterization of the activity of three natural products as potential antimicrobials against Staphylococcus aureus

Author(s): Shella Gilbert-Girard, Kirsi Savijoki, Jari Yli-Kauhaluoma, Adyary Fallarero
Published in: Abstract, Poster/Nordic Natural Products Conference, Flúðir, Iceland, 2019, 2019
Publisher: Nordic Natural Products Conference

Biofilm inhibiting effects of two Dehydroabietic acid derivatives on clinically relevant surfaces in the presence of host cells

Author(s): I. Reigada; K. Savijoki; J. Yli Kauhaluoma; A. Fallarero
Published in: Abstract, Poster/Nordic Natural Product Conference (NNPC), Flúðir, Iceland, 2019, 2019
Publisher: Nordic Natural Product Conference (NNPC)

Strategies to prevent biomaterials from Staphylococcus aureus infections effect of dehydroabietic acic derivatives on biomaterials pre-incubated with human cells

Author(s): I. Reigada; K. Savijoki; J. Yli Kauhaluoma; A. Fallarero
Published in: Abstract, Oral Presentation/eCM XIX: Orthopaedic Infection, Davos, Switzerland 2019, 2019, Page(s) 16
Publisher: eCM XIX: Orthopaedic Infection, Davos, Switzerland

A simplified process for producing test samples for VAP model using Additive Manufacturing

Author(s): B. Shaqour, J. Aizawa, E. Choińska, Z. Górecka, B. Verleije, K. Beyers, W. Święszkowski, P. Cos
Published in: Abstract, Poster/In: 30th Annual Conference of the European Society for Biomaterials. Dresden, Germany. Sept. 9-13, 2019, 2019
Publisher: European Society for Biomaterials

Comparison between physical and solvent mixing in FFF 3D printed drug loaded devices

Author(s): B. Shaqour, E. Choińska, Ż. Górecka, B. Verleije, K. Beyers, W. Święszkowski, P. Cos
Published in: Abstract, Poster/2019 eCM XIX: Orthopaedic Infection, Davos, Switzerland. June 26-28, 2019, 2019, Page(s) P28
Publisher: eCM XIX: Orthopaedic Infection

The effect of fosmidomycin prodrugs against Acinetobacter baumannii biofilms

Author(s): Frits van Charante, Charlotte Courtens, Serge van Calenbergh, Tom Coenye
Published in: Abstract, Poster/BSM (Belgian Society for Microbiology) conference 2019 (Brussels, Belgium), 2019
Publisher: Belgian Society for Microbiology

Why we need minimum information guidelines

Author(s): Jontana Allkja
Published in: Invited oral presentation/Eurobiofilms 2019, 2019
Publisher: Eurobiofilms

The use of fosmidomycin prodrugs against Acinetobacter baumannii in 3D-printed wound dressing

Author(s): Frits van Charante, David Martinez Perez, Charlotte Courtens, Wojciech Swieszkowski, Serge Van Calenbergh, Tom Coenye
Published in: Abstract, Oral Presentation/Print-Aid Outreach Conference, 2020
Publisher: Print-Aid

Identification and characterization of fingolimod as a repurposed antibacterial compound

Author(s): S. Gilbert-Girard, K. Savijoki, J. Yli-Kauhaluoma, A. Fallarero
Published in: Abstract, Oral Presentation/Print-Aid Outreach Conference, 2020
Publisher: Print-Aid

3D printed hybrid antimicrobial coating for prevention of implant-associated osteomyelitis

Author(s): D. Martínez Pérez, C. Guarch Pérez, I. Reigada, A. Fallarero, E. Choińska, S.A.J. Zaat, W. Święszkowski
Published in: Abstract, Oral Presentation/eCM XIX: Orthopaedic Infection, Davos, Switzerland 2019, 2019, Page(s) 19
Publisher: eCM Online Periodical

Effect of a dehydroabietic acic derivative on the prevention of biofilm formation in biomaterials pre-conditioned serum proteins in a co-culture system including SaOS-2 cells and clinical and collection strains of Staphylococcus aureus

Author(s): I. REIGADA, R. PÉREZ-TANOIRA, J.Z. PATEL, K. SAVIJOKI, J. YLI-KAUHALUOMA , T. J. KINNARI AND A. FALLARERO
Published in: Abstract, Oral Presentation/Print-Aid Outreach Conference, 2020
Publisher: Print-Aid

Peer reviewed articles (8)

Screening of FDA-Approved Drugs Using a 384-Well Plate-Based Biofilm Platform: The Case of Fingolimod

Author(s): Shella Gilbert-Girard, Kirsi Savijoki, Jari Yli-Kauhaluoma, Adyary Fallarero
Published in: Microorganisms, Issue 8/11, 2020, Page(s) 1834, ISSN 2076-2607
Publisher: MDPI, Microorganisms
DOI: 10.3390/microorganisms8111834

Non-leaching, Highly Biocompatible Nanocellulose Surfaces That Efficiently Resist Fouling by Bacteria in an Artificial Dermis Model

Author(s): Ghada Hassan, Nina Forsman, Xing Wan, Leena Keurulainen, Luis M. Bimbo, Susanne Stehl, Frits van Charante, Michael Chrubasik, Aruna S. Prakash, Leena-Sisko Johansson, Declan C. Mullen, Blair F. Johnston, Ralf Zimmermann, Carsten Werner, Jari Yli-Kauhaluoma, Tom Coenye, Per E. J. Saris, Monika Österberg, Vânia M. Moreira
Published in: ACS Applied Bio Materials, Issue 3/7, 2020, Page(s) 4095-4108, ISSN 2576-6422
Publisher: ACS
DOI: 10.1021/acsabm.0c00203

3D-Printed Drug Delivery Systems: The Effects of Drug Incorporation Methods on Their Release and Antibacterial Efficiency

Author(s): Bahaa Shaqour, Inés Reigada, Żaneta Górecka, Emilia Choińska, Bart Verleije, Koen Beyers, Wojciech Święszkowski, Adyary Fallarero, Paul Cos
Published in: Materials, Issue 13/15, 2020, Page(s) 3364, ISSN 1996-1944
Publisher: MDPI Open Access Publishing
DOI: 10.3390/MA13153364

Strategies to Prevent Biofilm Infections on Biomaterials: Effect of Novel Naturally-Derived Biofilm Inhibitors on a Competitive Colonization Model of Titanium by Staphylococcus aureus and SaOS-2 Cells

Author(s): Inés Reigada, Ramón Pérez-Tanoira, Jayendra Z. Patel, Kirsi Savijoki, Jari Yli-Kauhaluoma, Teemu J. Kinnari, Adyary Fallarero
Published in: Microorganisms, Issue 8/3, 2020, Page(s) 345, ISSN 2076-2607
Publisher: MDPI
DOI: 10.3390/microorganisms8030345

Minimum information guideline for spectrophotometric and fluorometric methods to assess biofilm formation in microplates

Author(s): Jontana Allkja, Thomas Bjarnsholt, Tom Coenye, Paul Cos, Adyary Fallarero, Joe J. Harrison, Susana P. Lopes, Antonio Oliver, Maria Olivia Pereira, Gordon Ramage, Mark E. Shirtliff, Paul Stoodley, Jeremy S. Webb, Sebastian A.J. Zaat, Darla M. Goeres, Nuno Filipe Azevedo
Published in: Biofilm, Issue 2, 2020, Page(s) 100010, ISSN 2590-2075
Publisher: Elsvier
DOI: 10.1016/j.bioflm.2019.100010

Optimization of a High-Throughput 384-Well Plate-Based Screening Platform with Staphylococcus aureus ATCC 25923 and Pseudomonas aeruginosa ATCC 15442 Biofilms

Author(s): Shella Gilbert-Girard, Kirsi Savijoki, Jari Yli-Kauhaluoma, Adyary Fallarero
Published in: International Journal of Molecular Sciences, Issue 21/9, 2020, Page(s) 3034, ISSN 1422-0067
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms21093034

Production of Drug Delivery Systems Using Fused Filament Fabrication: A Systematic Review

Author(s): Bahaa Shaqour, Aseel Samaro, Bart Verleije, Koen Beyers, Chris Vervaet and Paul Cos
Published in: Pharmaceutics, 2020, ISSN 1999-4923
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/pharmaceutics12060517

Combined Effect of Naturally-Derived Biofilm Inhibitors and Differentiated HL-60 Cells in the Prevention of Staphylococcus aureus Biofilm Formation

Author(s): Inés Reigada, Clara Guarch-Pérez, Jayendra Z. Patel, Martijn Riool, Kirsi Savijoki, Jari Yli-Kauhaluoma, Sebastian A. J. Zaat, Adyary Fallarero
Published in: Microorganisms, Issue 8/11, 2020, Page(s) 1757, ISSN 2076-2607
Publisher: MDPI, Microorganisms
DOI: 10.3390/microorganisms8111757

Other (1)

Orthogonal Approaches for Studying Bacterial Biofilms using Photometric Detection with Thermo Scientific Multiskan Sky and Brightfield Imaging with EVOS FL Auto Imaging System

Author(s): Inés Reigada, Ilkka Miettinen, Kirsi Savijoki, Adyary Fallarero
Published in: Application note, Thermo Fisher Scientific, 2018
Publisher: Thermo Fisher Scientific