Objective We propose the development of a groundbreaking technology platform that, for the first time, integrates nuclear magnetic resonance metabolomics and micro-imaging with microfluidic perfusion tissue slice culture. This will revolutionise life science research with unprecedented local insight into life processes in intact tissues under highly controlled conditions. We focus on liver tissue slice culture, with the immediate target of elucidating the mechanism of liver damage by drug-induced cholestasis. In the long term, the new technology will find wide application in other tissues, including intestinal, pancreatic, and brain slices. It will form the foundation of a new approach in the life sciences, allowing the detailed metabolicstudy of tissues at the system level.Liver disease is a significant and growing public health problem: 29 million people currently suffer from a serious liver condition in the EU. While the causes for some liver conditions are known, the mechanism of liver damage is generally poorly understood, largely due to the difficulty of studying live liver tissue at the systemic level. The proposed comprehensive research programme leads to a new technological platform for microfluidic tissue slice culture with direct observation of tissue metabolism and transport processes through nuclear magnetic resonance.It joins the expertise and creativity of four leading academic groups and one SME representing the disciplines of micro-engineering, physical chemistry, magnetic resonance, biochemistry, toxicology, and clinical hepatology across three institutions from three EU countries. Due to its high level of interdisciplinary integration, TISuMR is uniquely able to provide emerging researchers with a career springboard.TISuMR will have a profound impact on wider society by providing alternatives to animal testing, by increasing the efficiency and specificity of drug safety testing, and by enabling new treatments in the management of liver disease. Fields of science natural sciencesphysical sciencesclassical mechanicsfluid mechanicsmicrofluidicsnatural sciencesphysical sciencesopticsspectroscopyabsorption spectroscopymedical and health sciencesclinical medicinehepatologynatural sciencesmathematicspure mathematicsgeometrynatural scienceschemical sciencesphysical chemistry Keywords Nuclear Magnetic Resonance Tissue Slice Culture Liver Pathology Programme(s) H2020-EU.1.2. - EXCELLENT SCIENCE - Future and Emerging Technologies (FET) Main Programme H2020-EU.1.2.1. - FET Open Topic(s) FETOPEN-01-2016-2017 - FET-Open research and innovation actions Call for proposal H2020-FETOPEN-2016-2017 See other projects for this call Sub call H2020-FETOPEN-1-2016-2017 Funding Scheme RIA - Research and Innovation action Coordinator UNIVERSITY OF SOUTHAMPTON Net EU contribution € 1 256 126,25 Address Highfield SO17 1BJ Southampton United Kingdom See on map Region South East (England) Hampshire and Isle of Wight Southampton Activity type Higher or Secondary Education Establishments Links Contact the organisation Opens in new window Website Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Total cost € 1 256 126,25 Participants (2) Sort alphabetically Sort by Net EU contribution Expand all Collapse all RIJKSUNIVERSITEIT GRONINGEN Netherlands Net EU contribution € 935 121,25 Address Broerstraat 5 9712CP Groningen See on map Region Noord-Nederland Groningen Overig Groningen Activity type Higher or Secondary Education Establishments Links Contact the organisation Opens in new window Website Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Total cost € 935 121,25 KARLSRUHER INSTITUT FUER TECHNOLOGIE Germany Net EU contribution € 947 185,00 Address KAISERSTRASSE 12 76131 Karlsruhe See on map Region Baden-Württemberg Karlsruhe Karlsruhe, Stadtkreis Activity type Higher or Secondary Education Establishments Links Contact the organisation Opens in new window Website Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Total cost € 947 185,00