Integrated Tissue Slice Culture and NMR Metabolomics – A Novel Approach Towards Systemic Understanding of Liver Function And Disease | TISuMR | Project | Results | H2020 | CORDIS

Resultado final

Protocols for exposure of liver slices to 2-3 selected cholestatic drugs

Standard protocols for inducing cholestasis to be used by all project partners (drugs to be used, concentrations, exposure times, etc)

Metabolomic signatures of healthy and cholestatic tissue slices by NMR and complementary techniques

Metabolomic characterisation of the effects of druginduced cholestasis by NMR using the novel perfusionNMR devices and comparison to data from conventional techniques

Feasibility of micro-NMR as stand-alone technique for acquisition of metabolomic signatures of liver tissue

Demonstration of NMR metabolomic characterisation of PCLS

Selection of protocols for tissue harvest and incubation

Definition of best practice protocols for deployment to project partners in Southampton and Karlsruhe

Report on project symposium 1

Overview of project symposium, list of presentations, and select presentation materials

Final hypothesis for mechanism of liver damage by drug-induced cholestasis

Publication of known facts and resulting hypothesis on the mechanism of liver damage by druginduced cholestasis

Information on changes in composition of primary bile during cholestasis

Characterisation of the ability of the new NMR tools to provide information on bile composition in PCLS and its changes upon cholestasis

Report on viability and functionality of PCLS in novel NMR/perfusion systems

Detailed report comparing viability and functionality of liver slices in the static NMRperfusion system and the LoD perfusion device

Functional characterisation of cholestatic effects by conventional methods

quantification of the signature effects of the selected cholestatic drugs on PCLS by nonNMR means such as mRNA expression assays HPLC bile acid assays

Information on spatial distribution of cholestatic events in the liver acinus

Characterisation of the ability of the novel NMR protocols to provide spatial information on the distribution of cholestatic damage in liver tissue

Optimised TLP-NMR System

publication describing transmission line probe/perfusion system, its basic operation, and performance parameters

Project Identity

Project logo designed, website online, and twitter account established

Press releases

Press releases on discoveries, activities, and progress to be fielded about every 4 months, or as opportunity arises

Project website

Project website up and running; to be updated continuously

Publicaciones

Real‐Time NMR Monitoring of Spatially Segregated Enzymatic Reactions in Multilayered Hydrogel Assemblies**

Autores: Nurdiana Nordin, Lorenzo Bordonali, Hossein Davoodi, Novindi Dwi Ratnawati, Gudrun Gygli, Jan G. Korvink, Vlad Badilita, Neil MacKinnon
Publicado en: Angewandte Chemie International Edition, Edición 60/35, 2021, Página(s) 19176-19182, ISSN 1433-7851
Editor: John Wiley & Sons Ltd.
DOI: 10.1002/anie.202103585

Topologically optimized magnetic lens for magnetic resonance applications

Autores: Sagar Wadhwa, Mazin Jouda, Yongbo Deng, Omar Nassar, Dario Mager, Jan G. Korvink
Publicado en: Magnetic Resonance, Edición 1/2, 2020, Página(s) 225-236, ISSN 2699-0016
Editor: Groupement AMPERE
DOI: 10.5194/mr-1-225-2020

Glassy carbon microelectrodes minimize induced voltages, mechanical vibrations, and artifacts in magnetic resonance imaging

Autores: Surabhi Nimbalkar, Erwin Fuhrer, Pedro Silva, Tri Nguyen, Martin Sereno, Sam Kassegne, Jan Korvink
Publicado en: Microsystems & Nanoengineering, Edición 5/1, 2019, Página(s) Article 61 pp.1-11, ISSN 2055-7434
Editor: Springer Nature with the Areospace Information Research Institute, Chinese Academy of Sciences
DOI: 10.1038/s41378-019-0106-x

Time-resolved non-invasive metabolomic monitoring of a single cancer spheroid by microfluidic NMR

Autores: Bishnubrata Patra, Manvendra Sharma, William Hale, Marcel Utz
Publicado en: Scientific Reports, Edición 11/1, 2021, Página(s) pp.1-11, ISSN 2045-2322
Editor: Nature Publishing Group
DOI: 10.1038/s41598-020-79693-1

Direct Production of A Hyperpolarized Metabolite on a Microfluidic Chip

Autores: Sylwia J Barker, Laurynas Dagys, William Hale, Barbara Ripka, James Eills, Manvendra Sharma, Malcolm H Levitt, Marcel Utz
Publicado en: Analytical Chemistry, 2022, Página(s) in press, ISSN 1520-6882
Editor: American Chemical Society

An NMR-compatible microfluidic platform enabling in situ electrochemistry.

Autores: Hossein Davoodi; Nurdiana Nordin; Lorenzo Bordonali; Jan G. Korvink; Neil MacKinnon; Vlad Badilita
Publicado en: Lab on a Chip, Edición 2020/17, 2020, Página(s) pp.3202-3212, ISSN 1473-0197
Editor: Royal Society of Chemistry
DOI: 10.1039/d0lc00364f

Polyaramid-Based Flexible Antibacterial Coatings Fabricated Using Laser-Induced Carbonization and Copper Electroplating

Autores: Emil R Mamleyev, Fabian Falk, Peter G Weidler, Stefan Heissler, Sagar Wadhwa, Omar Nassar, CN Shyam Kumar, Christian Kübel, Christof Wöll, Monsur Islam, Dario Mager, Jan G Korvink
Publicado en: ACS Applied Materials & Interfaces, Edición 2021, 2021, Página(s) 53193-53205, ISSN 1944-8252
Editor: ACS
DOI: 10.1021/acsami.0c13058

Kinetic Investigation of a Cucurbit[7]uril-Based Pseudo[6]rotaxane System by Microfluidic NMR

Autores: Yibin Sun, Hongxun Fang, Xujing Lin, Xiuxiu Wang, Ganyu Chen, Xinchang Wang, Zhongqun Tian, Liulin Yang, Marcel Utz, Xiaoyu Cao
Publicado en: CCS Chemistry, 2021, Página(s) 1-26, ISSN 2096-5745
Editor: Chinese Chemical Society Publishing
DOI: 10.31635/ccschem.021.202100773

Microfluidic platform for serial mixing experiments with in operando nuclear magnetic resonance spectroscopy

Autores: Marek Plata, William Hale, Manvendra Sharma, Jörn M. Werner, Marcel Utz
Publicado en: Lab on a Chip, 2021, ISSN 1473-0197
Editor: Royal Society of Chemistry
DOI: 10.1039/d0lc01100b

Synergies between Hyperpolarized NMR and Microfluidics: A Review

Autores: Eills, J, Hale, W., Utz, M.
Publicado en: Progress in Nuclear Magnetic Resonance Spectroscopy, 2021, ISSN 0079-6565
Editor: Elsevier BV
DOI: 10.1016/j.pnmrs.2021.09.001

Wirelessly powered and remotely controlled valve-array for highly multiplexed analytical assay automation on a centrifugal microfluidic platform

Autores: Saraí M. Torres Delgado, David J. Kinahan, Lourdes Albina Nirupa Julius, Adam Mallette, David Sáenz Ardila, Rohit Mishra, Celina M. Miyazaki, Jan G. Korvink, Jens Ducrée, Dario Mager
Publicado en: Biosensors and Bioelectronics, Edición 109, 2018, Página(s) 214-223, ISSN 0956-5663
Editor: Pergamon Press Ltd.
DOI: 10.1016/j.bios.2018.03.012

High-Resolution Nuclear Magnetic Resonance Spectroscopy with Picomole Sensitivity by Hyperpolarization on a Chip

Autores: Eills, James; Hale, William; Sharma, Manvendra; Rossetto, Matheus; Levitt, Malcolm H.; Utz, Marcel
Publicado en: Journal of American Chemical Society, Edición 1, 2019, ISSN 0002-7863
Editor: American Chemical Society
DOI: 10.1021/jacs.9b03507

Topology optimization on two-dimensional manifolds

Autores: Yongbo Deng; Zhenyu Liu; Jan G. Korvink
Publicado en: Computer methods in applied mechanics and engineering, Edición 1, 2020, ISSN 0045-7825
Editor: Elsevier BV
DOI: 10.1016/j.cma.2020.112937

Leveraging 3D printing to enhance mass spectrometry: A review

Autores: Maciej Grajewski, Matthias Hermann, Richard D. Oleschuk, Elisabeth Verpoorte, Gert IJ. Salentijn
Publicado en: Analytica Chimica Acta, 2021, Página(s) 1-17, ISSN 0003-2670
Editor: Elsevier BV
DOI: 10.1016/j.aca.2021.338332

Probing the kinetics in supramolecular chemistry and molecular assembly by microfluidic-NMR spectroscopy

Autores: Hongxun Fang, Yibin Sun, Xinchang Wang, Manvendra Sharma, Zhong Chen, Xiaoyu Cao, Marcel Utz, Zhongqun Tian
Publicado en: Science China Chemistry, Edición 61/11, 2018, Página(s) 1460-1464, ISSN 1674-7291
Editor: Zhongguo Kexue Zazhishe/Science in China Press
DOI: 10.1007/s11426-018-9293-3

High-resolution nuclear magnetic resonance spectroscopy in microfluidic droplets

Autores: William Hale, Gabriel Rossetto, Rachael Greenhalgh, Graeme Finch, Marcel Utz
Publicado en: Lab on a Chip, Edición 18/19, 2018, Página(s) 3018-3024, ISSN 1473-0197
Editor: Royal Society of Chemistry
DOI: 10.1039/c8lc00712h

The eLoaD platform endows centrifugal microfluidics with on-disc power and communication

Autores: Saraí M. Torres Delgado, Jan G. Korvink, Dario Mager
Publicado en: Biosensors and Bioelectronics, Edición 117, 2018, Página(s) 464-473, ISSN 0956-5663
Editor: Pergamon Press Ltd.
DOI: 10.1016/j.bios.2018.05.056

Modular transmission line probes for microfluidic nuclear magnetic resonance spectroscopy and imaging

Autores: Manvendra Sharma, Marcel Utz
Publicado en: Journal of Magnetic Resonance, Edición 303, 2019, Página(s) 75-81, ISSN 1090-7807
Editor: Academic Press
DOI: 10.1016/j.jmr.2019.04.007

A multi-purpose, rolled-up, double-helix resonator

Autores: Pedro F. Silva, Saraí M. Torres Delgado, Mazin Jouda, Dario Mager, Jan G. Korvink
Publicado en: Journal of Magnetic Resonance, Edición 309, 2019, Página(s) 106599, ISSN 1090-7807
Editor: Academic Press
DOI: 10.1016/j.jmr.2019.106599

Automatic Adaptive Gain for Magnetic Resonance Sensitivity Enhancement

Autores: Mazin Jouda, Erwin Fuhrer, Pedro Silva, Jan G. Korvink, Neil MacKinnon
Publicado en: Analytical Chemistry, Edición 91/3, 2019, Página(s) 2376-2383, ISSN 0003-2700
Editor: American Chemical Society
DOI: 10.1021/acs.analchem.8b05148

“Small is beautiful” in NMR

Autores: Jan G. Korvink, Neil MacKinnon, Vlad Badilita, Mazin Jouda
Publicado en: Journal of Magnetic Resonance, Edición 306, 2019, Página(s) 112-117, ISSN 1090-7807
Editor: Academic Press
DOI: 10.1016/j.jmr.2019.07.012

Broadband and multi-resonant sensors for NMR

Autores: Hossein Davoodi, Mazin Jouda, Jan G. Korvink, Neil MacKinnon, Vlad Badilita
Publicado en: Progress in Nuclear Magnetic Resonance Spectroscopy, Edición 112-113, 2019, Página(s) 34-54, ISSN 0079-6565
Editor: Elsevier BV
DOI: 10.1016/j.pnmrs.2019.05.001

Gradient-induced mechanical vibration of neural interfaces during MRI

Autores: Erwin Fuhrer, Mazin Jouda, Christopher O. Klein, Manfred Wilhelm, Jan G. Korvink
Publicado en: IEEE Transactions on Biomedical Engineering, 2019, Página(s) 1-1, ISSN 0018-9294
Editor: Institute of Electrical and Electronics Engineers
DOI: 10.1109/tbme.2019.2923693

Parahydrogen based NMR hyperpolarisation goes micro: an alveolus for small molecule chemosensing

Autores: Lorenzo Bordonali, Nurdiana Nordin, Erwin Fuhrer, Neil MacKinnon, Jan G. Korvink
Publicado en: Lab on a Chip, Edición 19/3, 2019, Página(s) 503-512, ISSN 1473-0197
Editor: Royal Society of Chemistry
DOI: 10.1039/c8lc01259h

Micro-textures inversely designed with overlayed-lithography manufacturability for wetting behavior in Cassie–Baxter status

Autores: Yongbo Deng, Zhenyu Liu, Yasi Wang, Huigao Duan, Jan G. Korvink
Publicado en: Applied Mathematical Modelling, Edición 74, 2019, Página(s) 621-640, ISSN 0307-904X
Editor: Elsevier BV
DOI: 10.1016/j.apm.2019.04.059

Selective excitation enables encoding and measurement of multiple diffusion parameters in a single experiment

Autores: Neil MacKinnon, Mehrdad Alinaghian, Pedro Silva, Thomas Gloge, Burkhard Luy, Mazin Jouda, Jan G Korvink
Publicado en: Magnetic Resonance, Edición 2021, 2021, Página(s) 835-842, ISSN 2699-0016
Editor: Copernicus
DOI: 10.5194/mr-2-835-2021

ArduiTaM: accurate and inexpensive NMR auto tune and match system

Autores: Mazin Jouda, Saraí M. Torres Delgado, Mehrdad Alinaghian Jouzdani, Dario Mager, Jan G. Korvink
Publicado en: Magnetic Resonance, Edición 1/1, 2020, Página(s) 105-113, ISSN 2699-0016
Editor: Groupement AMPERE
DOI: 10.5194/mr-1-105-2020

Microfluidic Overhauser DNP chip for signal-enhanced compact NMR

Autores: Sebastian Z. Kiss, Neil MacKinnon, Jan G. Korvink
Publicado en: Scientific Reports, Edición 11/1, 2021, Página(s) pp.1-11, ISSN 2045-2322
Editor: Nature Publishing Group
DOI: 10.1038/s41598-021-83625-y

Integrated impedance sensing of liquid sample plug flow enables automated high throughput NMR spectroscopy

Autores: Omar Nassar, Mazin Jouda, Michael Rapp, Dario Mager, Jan G. Korvink, Neil MacKinnon
Publicado en: Microsystems & Nanoengineering, Edición 7/1, 2021, Página(s) pp.1-17, ISSN 2055-7434
Editor: Nature Portfolio
DOI: 10.1038/s41378-021-00253-2

Nanoantennas Inversely Designed to Couple Free Space and a Metal–Insulator–Metal Waveguide

Autores: Yeming Han, Yu Lin, Wei Ma, Jan G Korvink, Huigao Duan, Yongbo Deng
Publicado en: Nanomaterials, Edición 2021, 2021, Página(s) 3219, ISSN 2079-4991
Editor: MDPI
DOI: 10.3390/nano11123219

Fiber bundle topology optimization of hierarchical microtextures for wetting behavior in Cassie-Baxter mode

Autores: Yongbo Deng, Weihong Zhang, Zhenyu Liu, Jihong Zhu, Jan G Korvink
Publicado en: Structural and Multidisciplinary Optimization, Edición 61, 2020, Página(s) 2523-2556, ISSN 1615-1488
Editor: Springer
DOI: 10.1007/s00158-020-02558-8

Optical gauge head to evaluate gradient field induced vibrations of conductive structures during MRI

Autores: E. Fuhrer, M. Jouda, O. G. Gruschke, J. G. Korvink
Publicado en: 2017 International Conference on Electromagnetics in Advanced Applications (ICEAA), Edición 17244572, 2017, Página(s) 1555-1558, ISBN 978-1-5090-4451-1
Editor: IEEE
DOI: 10.1109/iceaa.2017.8065581

Wireless closed-loop control of centrifugo-pneumatic valving towards large-scale microfluidic process integration

Autores: David J. Kinahan, Sarai M. Delgado, Lourdes A.N. Julius, Adam Mallette, David Saenz-Ardila, Rohit Mishra, Celina M. Miyazaki, Jan Korvink, Dario Mager, Jens Ducree
Publicado en: 2018 IEEE Micro Electro Mechanical Systems (MEMS), Edición 17733066, 2018, Página(s) 1213-1216, ISBN 978-1-5386-4782-0
Editor: IEEE
DOI: 10.1109/memsys.2018.8346781

Microfluidics featuring multilayered hydrogel assemblies enable real-time NMR-monitoring of enzyme cascade reactions

Autores: Nurdiana Nordin, Hossein Davoodi, Novindi Dwi Ratnawati, Gudrun Gygli, Jan G Korvink, Vlad Badilita, Neil MacKinnon
Publicado en: ChemRxiv, Edición 00000000, 2021, ISSN 0000-0000
Editor: ACS

Ex Vivo Model in Cholestasis Research

Autores: Ruby E. H. Karsten, Dorenda Oosterhuis, Louise A. van Wijk, Peter Olinga
Publicado en: Experimental Cholestasis Research, Edición 1981, 2019, Página(s) 351-362, ISBN 978-1-4939-9419-9
Editor: Springer New York
DOI: 10.1007/978-1-4939-9420-5_23

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Integrated Tissue Slice Culture and NMR Metabolomics – A Novel Approach Towards Systemic Understanding of Liver Function And Disease

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