Objective THERE IS INCREASING EVIDENCE THAT THE ACTIVITIES OF GENETICALLY-DETERMINED DNA REPAIR FUNCTIONS ARE MAJOR FACTORS INFLUENCING THE RESPONSE OF MAMMALIAN CELLS TO IONIZING RADIATION. MUTATION AND RECOMBINATION ARE IMPORTANT RESPONSES TO RADIATION DAMAGE: THEY MAY LEAD TO GENETIC DISORDERS IN MAN AS WELL AS PREDISPOSITION TO A VARIETY OF HARMFUL AGENTS. DURING THE LAST FEW YEARS THERE HAVE BEEN REMARKABLE ADVANCES IN RECOMBINANT DNA TECHNIQUES WHICH MAY BE APPLIED TO THE STUDY OF REPAIR PROCESSES AND THE ANALYSIS OF MUTATION AND RECOMBINATION. IN THE PROPOSALS GIVEN BELOW GENE TRANSFER AND RECOMBINANT DNA TECHNIQUES WILL BE USED TO STUDY MOLECULAR ASPECTS OF RADIATION RESPONSE IN CULTURED MAMMALIAN CELLS. Studies being carried out concern:the molecular basis of mutation and recombination in mammalian cells differing in capacity to repair radiation damage;the isolation and cellular and molecular characterisation of repair deficient mammalian cell lines;the cloning and analysis of radiation repair genes from lower organisms and their introduction into mammalian cells.1 - THE MOLECULAR BASIS OF MUTATION AND RECOMBINATION IN MAMMALIAN CELLS DIFFERING IN CAPACITY TO REPAIR RADIATION DAMAGE. 2 - THE ISOLATION AND CALLULAR AND MOLECULAR CHARACTERISATION OF REPAIR-DEFICIENT MAMMALIAN CELL LINES. 3 - THE CLONING AND ANALYSIS OF RADIATION REPAIR GENES FROM LOWER ORGANISMS AND THEIR INTRODUCTION INTO MAMMALIAN CELLS. Fields of science medical and health sciencesmedical biotechnologygenetic engineeringgene therapynatural sciencesbiological sciencesgeneticsDNAnatural sciencesphysical sciencesnuclear physicsnatural sciencesbiological sciencesgeneticsmutation Programme(s) FP1-RADPROT 6C - Multiannual research and training programme (Euratom) in the field of radiation protection, 1985-1989 Topic(s) Data not available Call for proposal Data not available Funding Scheme CSC - Cost-sharing contracts Coordinator Medical Research Council (MRC) EU contribution No data Address 20 Park Crescent W1N 4AL London United Kingdom See on map Total cost No data
