Cel The AP-1 family of transcription factors participates in almost any program of gene expression, in cell proliferation, in apoptosis, in numerous types of differentiation, in cancer and autoimmune disease. Enhanced understanding of AP-1 function has significant scientific benefit and important implications for the development of inhibitory drugs and technological innovations in the healthcare industry. It is of utmost importance to dissect the molecular functions of the AP-1 factors, to study how physiologic regulators affect these functions and to then design strategies of intervention for appropriate pathologies. Due to the complexity of the AP-1 family and the need to dissect the specific role of single members of the family by mouse genetics it is mandatory to approach this theme as a collaboration of several laboratories. The participant to this research-training network together have already generated gene disruption of most of the AP-1 members and characterized their phenotypes. The availability of mouse models and highly informative in vitro systems to study specific aspects of physiological and pathological processes represent a unique tool to fully understand the role of each member of the family. Objectives of the present proposal are the dissection of AP-1 function with the aim of mouse genetics and in vitro analysis of cell mutants. First, the generation of a second generation of mutant and transgenic animals to answer to specific questions raised by the increased knowledge. Second, derive specific primary cell and cell lines lacking single AP-1 genes or expressing specific AP- I dimmers. Third, analyze in reconstituted in vitro models the role of AP-1 factors in differentiation pathways cell cycle progression or cell transformation. Fourth, identification of new AP-1 target genes by gene expression analysis performed with mutant cell clones using the micro array technology and with proteomic analysis. Fifth, functional analysis of relevant Dziedzina nauki natural sciencesbiological sciencesgeneticsmedical and health sciencesbasic medicineimmunologyautoimmune diseasesmedical and health sciencesclinical medicineoncologymedical and health sciencesbasic medicinepathologynatural sciencesmathematicspure mathematicsmathematical analysisfunctional analysis Program(-y) FP5-HUMAN POTENTIAL - Programme for research, technological development and demonstration on "Improving the human research potential and the socio-economic knowledge base" (1998-2002) Temat(-y) Data not available Zaproszenie do składania wniosków Data not available System finansowania NET - Research network contracts Koordynator UNIVERSITÀ DEGLI STUDI DI SIENA Wkład UE Brak danych Adres Via Fiorentina 1 53100 SIENA Włochy Zobacz na mapie Koszt całkowity Brak danych Uczestnicy (8) Sortuj alfabetycznie Sortuj według wkładu UE Rozwiń wszystko Zwiń wszystko CHRISTIE HOSPITAL (NHS) TRUST Zjednoczone Królestwo Wkład UE Brak danych Adres Wilmslow Road M20 4BX MANCHESTER Zobacz na mapie Koszt całkowity Brak danych FORSCHUNGSZENTRUM KARLSRUHE GMBH - TECHNIK UND UMWELT Niemcy Wkład UE Brak danych Adres Hermann von Helmholtz Platz 1 76021 KARLSRUHE Zobacz na mapie Koszt całkowity Brak danych GERMAN CANCER RESEARCH CENTER Niemcy Wkład UE Brak danych Adres 280,Im Neuenheimer Feld 280 69120 HEIDELBERG Zobacz na mapie Koszt całkowity Brak danych INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE Francja Wkład UE Brak danych Adres 46,Allée d'Italie 46 ADR 5 - Rhône-Alpes, Auvergne, B 69364 Lyon Zobacz na mapie Koszt całkowity Brak danych INSTITUT PASTEUR Francja Wkład UE Brak danych Adres Rue du Docteur Roux 25 75724 PARIS Zobacz na mapie Koszt całkowity Brak danych KING'S COLLEGE LONDON Zjednoczone Królestwo Wkład UE Brak danych Adres STRAND WC2R 2LS LONDON Zobacz na mapie Koszt całkowity Brak danych LEIDEN UNIVERSITY Niderlandy Wkład UE Brak danych Adres Wassenaarseweg 72 2333 AL LEIDEN Zobacz na mapie Koszt całkowity Brak danych RESEARCH INSTITUTE OF MOLECULAR PATHOLOGY Austria Wkład UE Brak danych Adres 7,DR. BOHR-GASSE 7 1030 WIEN Zobacz na mapie Koszt całkowity Brak danych
