Obiettivo The requirement to screen for known SNPs (single nucleotide polymorphisms) has become one of the key challenges to be addressed to enable the exploitation of the human genome sequence, where approximately 3 million SNPs are responsible for all of the variation within the human population. Several methods and technologies are currently available for detection of SNPs, but no single platform exists which can deliver 100% accuracy, in a low cost, versatile, and easy-to-use integrated system.The proposed research will focus on the development of integrated SNP detection platforms to include modules for DNA extraction and purification from biological samples, DNA amplification, DNA characterisation (including SNP detection), signal transduction, interpretation and data analysis. Integration of the modules on a single platform will include a transport mechanism based on electrowetting actuation (EWOD).Two alternatives SNP detection platforms will be developed based on optical and magnetic sensing respectively. The whole system will be packaged as a single automated functional system with a simplified GUI interface, based on specifications recommended by potential end-users within the consortium. This systems development will focus on delivering a product customised for low to medium throughput, low cost, point-of-care applications, with emphasis on providing very rapid and accurate results.Within the project, the systems will be benchmarked for screening of SNPs in the CFTR gene that are associated with cystic fibrosis. CF patient samples will be used to verify the accuracy and reproducibility of the system in a clinical diagnostics laboratory. Campo scientifico engineering and technologyelectrical engineering, electronic engineering, information engineeringelectronic engineeringcontrol systemsengineering and technologyelectrical engineering, electronic engineering, information engineeringelectronic engineeringsensorsoptical sensorsmedical and health sciencesbasic medicinepharmacology and pharmacypharmaceutical drugsnatural sciencesbiological sciencesgeneticsDNAnatural sciencesbiological sciencesgeneticsnucleotides Parole chiave Nanotechnology Programma(i) FP6-NMP - Nanotechnologies and nanosciences, knowledge-based multifunctional materials and new production processes and devices: thematic priority 3 under the 'Focusing and integrating community research' of the 'Integrating and strengthening the European Research Area' specific programme 2002-2006. Argomento(i) NMP-2003-3.4.1.5-2 - Ethical, legal, social aspects of research in nanotechnology NMP-2004-IST-NMP-2 - Bio-sensors for Diagnosis and Healthcare Invito a presentare proposte FP6-2004-IST-NMP-2 Vedi altri progetti per questo bando Meccanismo di finanziamento STREP - Specific Targeted Research Project Coordinatore UNIVERSITY COLLEGE CORK, NATIONAL UNIVERSITY OF IRELAND, CORK Contributo UE Nessun dato Indirizzo Lee Maltings, CORK Irlanda Mostra sulla mappa Collegamenti Sito web Opens in new window Costo totale Nessun dato Partecipanti (6) Classifica in ordine alfabetico Classifica per Contributo UE Espandi tutto Riduci tutto ASPER BIOTECH LTD Estonia Contributo UE Nessun dato Indirizzo Oru 3 TARTU Mostra sulla mappa Collegamenti Sito web Opens in new window Costo totale Nessun dato CHARLES UNIVERSITY PRAGUE Cechia Contributo UE Nessun dato Indirizzo Ovocny TRH 5 PRAGUE 1 Mostra sulla mappa Collegamenti Sito web Opens in new window Costo totale Nessun dato COMMISSARIAT A L'ENERGIE ATOMIQUE (CEA) Francia Contributo UE Nessun dato Indirizzo 31-33 rue de la Fédération PARIS Mostra sulla mappa Collegamenti Sito web Opens in new window Costo totale Nessun dato FUNDACAO DA FACULDADE DE CIENCIAS DA UNIVERSIDADE DE LISBOA Portogallo Contributo UE Nessun dato Indirizzo Campo Grande - Edificio C7 - 1 piso LISBOA Mostra sulla mappa Collegamenti Sito web Opens in new window Costo totale Nessun dato INSTITUTO DE ENGENHARIA DE SISTEMAS E COMPUTADORES PARA OS MICROSISTEMAS E AS NANOTECNOLOGIAS Portogallo Contributo UE Nessun dato Indirizzo Rua ALVES REDOL 9 LISBOA Mostra sulla mappa Collegamenti Sito web Opens in new window Costo totale Nessun dato NATIONAL UNIVERSITY OF IRELAND, DUBLIN. UNIVERSITY COLLEGE DUBLIN Irlanda Contributo UE Nessun dato Indirizzo Belfield DUBLIN Mostra sulla mappa Collegamenti Sito web Opens in new window Costo totale Nessun dato