Objetivo Normal placental angiogenesis is critical for embryonic survival and development. Epigenetic modifications, such as methylation of CpG islands, regulate the expression and imprinting of genes. Epigenetic abnormalities have been observed in embryos from assisted reproductive technologies (ART), which could explain the poor placental vascularisation, embryonic/fetal death, and altered fetal growth in these pregnancies. Both cloned (somatic cell nuclear transfer, or SNCT) and monoparental (parthogenotes, only maternal genes; androgenotes, only paternal genes) embryos provide important models for studying defects in expression and methylation status/imprinting of genes regulating placental function. Our hypothesis is that placental vascular development is compromised during early pregnancy in embryos from ART, in part due to altered expression or imprinting/methylation status of specific genes regulating placental angiogenesis. We will evaluate fetal growth, placental vascular growth, and expression and epigenetic status of genes regulating placental angiogenesis during early pregnancy in 3 Specific Aims: (1) after natural mating; (2) after transfer of biparental embryos from in vitro fertilization, and SCNT; and (3) after transfer of parthenogenetic or androgenetic embryos. These studies will therefore contribute substantially to our understanding of the regulation of placental development and vascularisation during early pregnancy, and could pinpoint the mechanism contributing to embryonic loss and developmental abnormalities in foetuses from ART. Any or all of these observations will contribute to our understanding of and also our ability to successfully employ ART, which are becoming very wide spread and important in human medicine as well as in animal production. Ámbito científico medical and health sciencesclinical medicinegynaecologyreproductive medicinemedical and health sciencesclinical medicineobstetricsfetal medicineagricultural sciencesanimal and dairy sciencedomestic animalsanimal husbandrymedical and health sciencesclinical medicineembryologynatural sciencesbiological sciencesgeneticsepigenetics Palabras clave assisted reproductive technologies cloning early pregnancy loss embryo imprinting in vitro fertilization methylation placental angiogenesis Programa(s) FP7-IDEAS-ERC - Specific programme: "Ideas" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Tema(s) ERC-SG-LS7 - Applied life sciences, biotechnology and bioengineering: agricultural, animal, fishery, forestry/food sciences; biotechnology, chemical biology, genetic engineering, synthetic biology, industrial biosciences; environmental biotechnology. Convocatoria de propuestas ERC-2007-StG Consulte otros proyectos de esta convocatoria Régimen de financiación ERC-SG - ERC Starting Grant Institución de acogida UNIVERSITA DEGLI STUDI DI TERAMO Aportación de la UE € 363 600,00 Dirección VIA RENATO BALZARINI 1 3 5 7 64100 Teramo Italia Ver en el mapa Región Sud Abruzzo Teramo Tipo de actividad Higher or Secondary Education Establishments Contacto administrativo Maria Luigia Scuteri (Dr.) Investigador principal Grazyna Ewa Ptak (Dr.) Enlaces Contactar con la organización Opens in new window Sitio web Opens in new window Coste total Sin datos Beneficiarios (1) Ordenar alfabéticamente Ordenar por aportación de la UE Ampliar todo Contraer todo UNIVERSITA DEGLI STUDI DI TERAMO Italia Aportación de la UE € 363 600,00 Dirección VIA RENATO BALZARINI 1 3 5 7 64100 Teramo Ver en el mapa Región Sud Abruzzo Teramo Tipo de actividad Higher or Secondary Education Establishments Contacto administrativo Maria Luigia Scuteri (Dr.) Investigador principal Grazyna Ewa Ptak (Dr.) Enlaces Contactar con la organización Opens in new window Sitio web Opens in new window Coste total Sin datos