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New systems for ultrafast isolation of veterinary drugs and contaminants in complex matrices by physical-chemical treatment

The proposed new methods are capable to reduce sample treatment time for the isolation of veterinary drugs and contaminants from complex matrices such as liver, kidney, thyroid gland, urine, saliva and serum proceeding from farm animals (pig, bovine, poultry) from hours to minutes.

The methods are based on a series of ultrafast subsequent steps, including liquid-liquid extraction, protein precipitation, defatting, solid phase extraction (the latter only employed for clean-up of strongly hydrophilic or lipohhilic compounds and some miscellaneous). Sample treatment using these methods does not require more than basic laboratory equipment (pipettes, blender, small centrifuge). The extracts, normally about 0.2 ml, can then be used for xenobiotics determination by GC, TLC or similar.,Kits for a variety of applications are available, including, but not limited to: - Clean-up system at pH 7 and 9 for basic veterinary drugs (some beta-agonists, tricyclic antidepressants, phenotiazines), neutral drugs (esteroides anabolics, corticosteroides, benzodiazepines), in urine, saliva serum.,- Protein precipitation based methods (at pH 7) for isolation of neutral drugs from muscle tissue and liver, strongly hydrophilic veterinary drugs (thyreostatic, salbutamol, terbutaline) from liver and thyroids, and miscellaneous drugs (sulfonamide antibacterials) from kidney.,- Protein precipitation based methods (at pH 9) for isolation of basic drugs (some beta-agonists, tricyclic antidepressants, phenotiazines) from liver and blood. ,- Methods including a Solid Phase Extraction step for clean-up and extraction of strongly hydrophilic drugs into an organic phase. Further applicable to miscellaneous drugs. The kits contain all the reactives and additional material (SPE cartridges, syringes, etc.) which are neccesary to carry out the extraction. A decantation accesory has to be once achieved apart and can be used with all commercial kits. Methods for extraction of polycyclic aromatic hydrocarbons, dibenzo-p-dioxins, hydrophilic antibiotics, organophosphorates, organochlorines, carbamates, pyrethrin, pyrethroids, triazines, etc. are in stage of development.