Conference in French: Recent development on transport of molecules across cell membranes
by Sandrine SAGAN
1 April 2019 - 1 April 2019
France
Complete title:
Recent development on transport of molecules across cell membranes, or how to turn a hydrophobic barrier into a membrane permeable to big and polar molecules without sophisticated transport systems
Abstract:
Membranes are present at all levels of organization of living organisms. Long presented as a passive barrier separating the cell from its environment, the plasma membrane plays a major role in cellular homeostasis by providing crucial dynamic functions of control and processing/transduction of information in response to numerous external stimuli. Despite its simple molecular composition (lipids, proteins, carbohydrates), its complex spatio-temporal organization provides the plasma membrane with very dynamic deformation, curvature and elasticity properties. Sophisticated active transport protein systems control the exchange across the membrane to import essential nutrients. Since about thirty years, it is known that large polar nanoobjects such as proteins or peptides are able to cross the plasma membrane without using the existing active transport systems. I will illustrate with recent examples the physicochemical aspects which make it possible to apprehend and unveil these new mechanisms of passage of the plasma membrane.
References:
• Walrant A, Cardon S, Burlina F, Sagan S. Membrane Crossing and Membranotropic Activity of Cell-Penetrating Peptides: Dangerous Liaisons? Acc Chem Res. 2017 Dec 19;50(12):2968-2975.
• Jiao CY, Sachon E, Alves ID, Chassaing G, Bolbach G, Sagan S. Exploiting Benzophenone photoreactivity To Probe the Phospholipid Environment and Insertion Depth of the Cell-Penetrating Peptide Penetratin in Model Membranes. Angew Chem Int Ed Engl. 2017 Jul 3;56(28):8226-8230.
• Bechara C, Pallerla M, Burlina F, Illien F, Cribier S, Sagan S. Massive glycosaminoglycan-dependent entry of Trp-containing cell-penetrating peptides induced by exogenous sphingomyelinase or cholesterol depletion. Cell Mol Life Sci. 2015 Feb;72(4):809-820.
• Bechara C, Sagan S. Cell-penetrating peptides: 20 years later, where do we stand? FEBS Lett. 2013 Jun 19; 587(12):1693-1702. doi:10.1016/j.febslet.2013.04.031
• Sagan S, Burlina F, Alves ID, Bechara C, Dupont E, Joliot A. Homeoproteins and homeoprotein-derived peptides: going in and out. Curr Pharm Des. 2013;19(16):2851-2862.
Recent development on transport of molecules across cell membranes, or how to turn a hydrophobic barrier into a membrane permeable to big and polar molecules without sophisticated transport systems
Abstract:
Membranes are present at all levels of organization of living organisms. Long presented as a passive barrier separating the cell from its environment, the plasma membrane plays a major role in cellular homeostasis by providing crucial dynamic functions of control and processing/transduction of information in response to numerous external stimuli. Despite its simple molecular composition (lipids, proteins, carbohydrates), its complex spatio-temporal organization provides the plasma membrane with very dynamic deformation, curvature and elasticity properties. Sophisticated active transport protein systems control the exchange across the membrane to import essential nutrients. Since about thirty years, it is known that large polar nanoobjects such as proteins or peptides are able to cross the plasma membrane without using the existing active transport systems. I will illustrate with recent examples the physicochemical aspects which make it possible to apprehend and unveil these new mechanisms of passage of the plasma membrane.
References:
• Walrant A, Cardon S, Burlina F, Sagan S. Membrane Crossing and Membranotropic Activity of Cell-Penetrating Peptides: Dangerous Liaisons? Acc Chem Res. 2017 Dec 19;50(12):2968-2975.
• Jiao CY, Sachon E, Alves ID, Chassaing G, Bolbach G, Sagan S. Exploiting Benzophenone photoreactivity To Probe the Phospholipid Environment and Insertion Depth of the Cell-Penetrating Peptide Penetratin in Model Membranes. Angew Chem Int Ed Engl. 2017 Jul 3;56(28):8226-8230.
• Bechara C, Pallerla M, Burlina F, Illien F, Cribier S, Sagan S. Massive glycosaminoglycan-dependent entry of Trp-containing cell-penetrating peptides induced by exogenous sphingomyelinase or cholesterol depletion. Cell Mol Life Sci. 2015 Feb;72(4):809-820.
• Bechara C, Sagan S. Cell-penetrating peptides: 20 years later, where do we stand? FEBS Lett. 2013 Jun 19; 587(12):1693-1702. doi:10.1016/j.febslet.2013.04.031
• Sagan S, Burlina F, Alves ID, Bechara C, Dupont E, Joliot A. Homeoproteins and homeoprotein-derived peptides: going in and out. Curr Pharm Des. 2013;19(16):2851-2862.
Keywords
Pharmacology, mass spectrometry, membrane proteins, molecular mechanisms, cell-penetrating peptides