Objective
DNA chip is a new technique in molecular biology, which has innumerable applications. The proposed project an interdisciplinary research involving DNA Chip technology and Marine Science. The objective of this project is to implement DNA chip array hybridization for identification of fish species, their eggs and larvae.
The ribosomal DNA (rDNA) of vertebrates is composed of three coding regions (18S, 5.8S and 28S) which evolve at relatively slow rates, external and internal transcribed spacers (5' ETS, ITSI, and ITS2) which evolve at faster rates, and their intergenic spacer (IGS) which evolve at a very rapid rate. A hypervariable region of the IGS has been identified just 3' to the 28S, which exhibits length variation in all salmonid species.
This aspect of D NA sequence variation of the IGS and ITS is taken into consideration for this study. At first the nucleotide sequence of the rDNA of 50 commercially important marine fish species found in the European waters, mainly the species belonging to the Cod, Haddock, Whiting, Sole, Hake, Eel and Herring will be cloned and sequenced.
In the rDNA sequence the variable and hypervariable regions of each species will be identified and the species specific oligonucleotides corresponding to that region will be chemically synthesized and using them a DNA chip will be constructed and validated.
The construction of DNA chip and the other related works will be done in European Molecular Biology Laboratory in Heidelberg, Germany in Dr. Ansorg's group that has good number of publications, patents in DNA microarray and also conducting a yearly training program on DNA microarray.
This technology has further use in Biodiversity research, Conservation studies, Population dynamics research, examination of adultered fish food etc. Undertaking the training program by the candidate would be a new area of research in Marine Biotechnology research and will foster further research collaborations with European scientist.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences genetics DNA
- natural sciences biological sciences zoology ichthyology
- natural sciences biological sciences genetics nucleotides
- natural sciences earth and related environmental sciences oceanography
- natural sciences biological sciences molecular biology
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2002-MOBILITY-7
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
IIF - Marie Curie actions-Incoming International Fellowships
Coordinator
HEIDELBERG
Germany
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.