DNA chip is a new technique in molecular biology, which has innumerable applications. The proposed project an interdisciplinary research involving DNA Chip technology and Marine Science. The objective of this project is to implement DNA chip array hybridization for identification of fish species, their eggs and larvae.
The ribosomal DNA (rDNA) of vertebrates is composed of three coding regions (18S, 5.8S and 28S) which evolve at relatively slow rates, external and internal transcribed spacers (5' ETS, ITSI, and ITS2) which evolve at faster rates, and their intergenic spacer (IGS) which evolve at a very rapid rate. A hypervariable region of the IGS has been identified just 3' to the 28S, which exhibits length variation in all salmonid species.
This aspect of D NA sequence variation of the IGS and ITS is taken into consideration for this study. At first the nucleotide sequence of the rDNA of 50 commercially important marine fish species found in the European waters, mainly the species belonging to the Cod, Haddock, Whiting, Sole, Hake, Eel and Herring will be cloned and sequenced.
In the rDNA sequence the variable and hypervariable regions of each species will be identified and the species specific oligonucleotides corresponding to that region will be chemically synthesized and using them a DNA chip will be constructed and validated.
The construction of DNA chip and the other related works will be done in European Molecular Biology Laboratory in Heidelberg, Germany in Dr. Ansorg's group that has good number of publications, patents in DNA microarray and also conducting a yearly training program on DNA microarray.
This technology has further use in Biodiversity research, Conservation studies, Population dynamics research, examination of adultered fish food etc. Undertaking the training program by the candidate would be a new area of research in Marine Biotechnology research and will foster further research collaborations with European scientist.
Fields of science
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