Objective
Amyloid precursor protein (APP) is genetically and biochemically linked to the pathogenesis of Alzheimer¿s disease (AD), the most common cause of senile dementia in industrialized countries. It has been proposed that reduction of the level or activity of c ertain APP fragments, in addition to accumulation of Abeta peptide, may play a critical role in the cognitive dysfunction associated with AD. To test this hypothesis, it is important to understand the roles that full-length APP and its fragments normally p lay in neuronal structure and function. Emerging evidence suggests that sAPP, the secreted N-terminal fragment of APP, can be considered as a signaling polypeptide that evokes coordinated responses in neuronal and some non-neuronal target cells. Prerequisi te for such functions is the existence of a membrane receptor for sAPP, which has been postulated but is at present unidentified. The aim of the proposed study is to identify sAPP receptors and characterize them biochemically. For this purpose, binding stu dies with recombinant sAPP will be performed with sections of brain and other tissues. In addition, the results of the tissue-binding approach will be confirmed by conducting binding studies with cultured cells. To be able to analyze the complex of sAPP wi th its receptor, the complex will be covalently linked via the photocrosslinking amino acid p-benzoyl-L.phenylalanine. Introduction of the photolabile amino acid at defined positions ensures that only direct binding partners are stably bound. Thereafter, t he complex will be purified and sAPP-bound proteins will be characterized by mass-spectrometrical methods.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- medical and health sciences basic medicine neurology dementia alzheimer
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences chemical sciences organic chemistry amines
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2002-MOBILITY-5
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
ZWIJNAARDE
Belgium
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.