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Combating 2019-nCoV: Advanced Nanobiosensing platforms for POC global diagnostics and surveillance

Deliverables

Public Report

Public report to inform about the results of the 6 first months of the project

Publications

Rapid and qualitative identification of SARS-CoV-2 mutationsassociated with variants of concern using a multiplex RT-PCR assaycoupled with melting analysis

Author(s): G. Sberna, L. Fabeni, G. Berno, F. Carletti, E. Specchiarello, F. Colavita, S. Meschi, G. Matusali, A. R. Garbuglia, L. Bordi, E. Lalle
Published in: International Journal of Infectious Diseases, 12019712, 2022, ISSN 1201-9712
Publisher: Elsevier BV
DOI: 10.1016/j.ijid.2022.06.032

Rueca M, Bartolini B, Gruber CEM, Piralla A, Baldanti F, Giombini E, Messina F, Marchioni L, Ippolito G, Di Caro A, Capobianchi MR.

Author(s): Compartmentalized Replication of SARS-Cov-2 in Upper vs. Lower Respiratory Tract Assessed by Whole Genome Quasispecies Analysis.
Published in: Microorganisms, 20762607, 2020, ISSN 2076-2607
Publisher: MDPI
DOI: 10.3390/microorganisms8091302

Trend of respiratory pathogens during the COVID-19 epidemic

Author(s): Giuseppe Sberna, Alessandra Amendola, Maria Beatrice Valli, Fabrizio Carletti, Maria Rosaria Capobianchi, Licia Bordi, Eleonora Lalle
Published in: Journal of Clinical Virology, 129, 2020, Page(s) 104470, ISSN 1386-6532
Publisher: Elsevier BV
DOI: 10.1016/j.jcv.2020.104470

Rapid and sensitive detection of SARS-CoV-2 RNA using the Simplexa™ COVID-19 direct assay

Author(s): Licia Bordi, Antonio Piralla, Eleonora Lalle, Federica Giardina, Francesca Colavita, Monica Tallarita, Giuseppe Sberna, Federica Novazzi, Silvia Meschi, Concetta Castilletti, Angela Brisci, Giulia Minnucci, Veronica Tettamanzi, Fausto Baldanti, Maria Rosaria Capobianchi
Published in: Journal of Clinical Virology, 128, 2020, Page(s) 104416, ISSN 1386-6532
Publisher: Elsevier BV
DOI: 10.1016/j.jcv.2020.104416

COVID-19 Rapid Antigen Test as Screening Strategy at Points of Entry: Experience in Lazio Region, Central Italy

Author(s): Colavita F, Vairo F, Meschi S, Valli MB, Lalle E, Castilletti C, Fusco D, Spiga G, Bartoletti P, Ursino S, Sanguinetti M, Di Caro A, Vaia F, Ippolito G, Capobianchi MR
Published in: Biomolecules, 2218273X, 2021, ISSN 2218-273X
Publisher: MDPI
DOI: 10.3390/biom11030425

SARS-CoV-2 Serum Neutralization Assay: A Traditional Tool for a Brand-New Virus

Author(s): Matusali G, Colavita F, Lapa D, Meschi S, Bordi L, Piselli P, Gagliardini R, Corpolongo A, Nicastri E, Antinori A, Ippolito G, Capobianchi MR, Castilletti C
Published in: Viruses, 19994915, 2021, ISSN 1999-4915
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/v13040655

Evidences for lipid involvement in SARS-CoV-2 cytopathogenesis

Author(s): Nardacci, R., Colavita, F., Castilletti, C. et al.
Published in: Cell Death Dis, 20414889, 2021, ISSN 2041-4889
Publisher: Nature Publishing Group
DOI: 10.1038/s41419-021-03527-9

Development and Evaluation of a duo SARS-CoV-2 RT-qPCR Assay Combining Two Assays Approved by the World Health Organization Targeting the Envelope and the RNA-Dependant RNA Polymerase (RdRp) Coding Regions

Author(s): Pezzi L, Charrel RN, Ninove L, Nougairede A, Molle G, Coutard B, Durand G, Leparc-Goffart I, de Lamballerie X, Thirion L.
Published in: Viruses, 19994915, 2020, ISSN 1999-4915
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/v12060686

Investigation of Nasal/Oropharyngeal Microbial Community of COVID-19 Patients by 16S rDNA Sequencing

Author(s): Rueca M, Fontana A, Bartolini B, Piselli P, Mazzarelli A, Copetti M, Binda E, Perri F, Gruber CEM, Nicastri E, Marchioni L, Ippolito G, Capobianchi MR, Di Caro A, Pazienza V.
Published in: Int J Environ Res Public Health, 16604601, 2021, ISSN 1660-4601
Publisher: MDPI
DOI: 10.3390/ijerph18042174

Engineering photonics solutions for COVID-19

Author(s): Maria Soler, Alexis Scholtz, Rene Zeto, Andrea M. Armani
Published in: APL Photonics, 5/9, 2020, Page(s) 090901, ISSN 2378-0967
Publisher: AIP Publishing
DOI: 10.1063/5.0021270

Virological Characterization of the First 2 COVID-19 Patients Diagnosed in Italy: Phylogenetic Analysis, Virus Shedding Profile From Different Body Sites, and Antibody Response Kinetics

Author(s): Colavita F, Lapa D, Carletti F, Lalle E, Messina F, Rueca M, Matusali G, Meschi S, Bordi L, Marsella P, Nicastri E, Marchioni L, Mariano A, Scorzolini L, Ascoli Bartoli T, Di Caro A, Ippolito G, Capobianchi MR, Castilletti C
Published in: Open Forum Infec Dis, 23288957, 2020, ISSN 2328-8957
Publisher: Oxford University Press
DOI: 10.1093/ofid/ofaa403

Heat Inactivation of Different Types of SARS-CoV-2 Samples: What Protocols for Biosafety, Molecular Detection and Serological Diagnostics?

Author(s): Pastorino B, Touret F, Gilles M, de Lamballerie X, Charrel RN
Published in: Viruses, 19994915, 2020, ISSN 1999-4915
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/v12070735

Saliva Is a Valid Alternative to Nasopharyngeal Swab in Chemiluminescence-Based Assay for Detection of SARS-CoV-2 Antigen

Author(s): Amendola A, Sberna G, Lalle E, Colavita F, Castilletti C, Menchinelli G, Posteraro B, Sanguinetti M, Ippolito G, Bordi L, Capobianchi MR
Published in: Journal of Clinical Medicine, 20770383, 2021, ISSN 2077-0383
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/jcm10071471

Emergence of Bat-Related Betacoronaviruses: Hazard and Risks

Author(s): Roger Frutos, Jordi Serra-Cobo, Lucile Pinault, Marc Lopez Roig, Christian A. Devaux
Published in: Frontiers in Microbiology, 12, 2021, ISSN 1664-302X
Publisher: Frontiers Media
DOI: 10.3389/fmicb.2021.591535

Evaluation of Chemical Protocols for Inactivating SARS-CoV-2 Infectious Samples

Author(s): Pastorino B, Touret F, Gilles M, Luciani L, de Lamballerie X, Charrel RN
Published in: Viruses, 19994915, 2020, ISSN 1999-4915
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/v12060624

Performance evaluation of Abbott ARCHITECT SARS-CoV-2 IgG immunoassay in comparison with indirect immunofluorescence and virus microneutralization test

Author(s): Silvia Meschi, Francesca Colavita, Licia Bordi, Giulia Matusali, Daniele Lapa, Alessandra Amendola, Francesco Vairo, Giuseppe Ippolito, Maria Rosaria Capobianchi, Concetta Castilletti
Published in: Journal of Clinical Virology, 129, 2020, Page(s) 104539, ISSN 1386-6532
Publisher: Elsevier BV
DOI: 10.1016/j.jcv.2020.104539

First Coronavirus Active Survey in Rodents From the Canary Islands

Author(s): Monastiri Abir, Martín-Carrillo Natalia, Foronda Pilar, Izquierdo-Rodríguez Elena, Feliu Carles, López-Roig Marc, Miquel Jordi, Ar Gouilh Meriadeg, Serra-Cobo Jordi
Published in: Frontiers in Veterinary Science, 22971769, 2021, ISSN 2297-1769
Publisher: Frontiers
DOI: 10.3389/fvets.2021.708079

Virological and Serological Characterisation of SARS-CoV-2 Infections Diagnosed After mRNA BNT162b2 Vaccination Between December 2020 and March 2021

Author(s): Colavita Francesca, Meschi Silvia, Gruber Cesare Ernesto Maria, Rueca Martina, Vairo Francesco, Matusali Giulia, Lapa Daniele, Giombini Emanuela, De Carli Gabriella, Spaziante Martina, Messina Francesco, Bonfiglio Giulia, Carletti Fabrizio, Lalle Eleonora, Fabeni Lavinia, Berno Giulia, Puro Vincenzo, Bartolini Barbara, Di Caro Antonino, Ippolito Giuseppe, Capobianchi Maria Rosaria, Castilletti Con
Published in: Frontiers in Medicine, 2296858X, 2021, ISSN 2296-858X
Publisher: Frontiers
DOI: 10.3389/fmed.2021.815870

Endonuclease-based genotyping of the RBM as a method to track the emergence or evolution of SARS-CoV-2 variants

Author(s): Lopez E, Barthélémy M, Baronti C, Masse S, Falchi A, Durbesson F, Vincentelli R, de Lamballerie X, Charrel R, Coutard B.
Published in: iScience, 25890042, 2021, ISSN 2589-0042
Publisher: Cell Press Elsevier
DOI: 10.1016/j.isci.2021.103329

Comparison of Allplex™ SARS-CoV-2 Assay, Easy SARS-CoV-2 WE and Lumipulse quantitative SARS-CoV-2 antigen test performance using automated systems for the diagnosis of COVID-19

Author(s): Sberna G, Basile F, Guarino ML, Capobianchi MR, Bordi L, Parisi G
Published in: International Journal of Infectious diseases, 12019712, 2021, ISSN 1201-9712
Publisher: Elsevier BV
DOI: 10.1016/j.ijid.2021.09.069

Nanophotonic biosensors for point-of-care COVID-19 diagnostics and coronavirus surveillance

Author(s): G. Ruiz-Vega, M. Soler, L. M. Lechuga
Published in: J Phys Photonics, 25157647, 2021, ISSN 2515-7647
Publisher: IOP Science
DOI: 10.1088/2515-7647/abd4ee

Rapid and direct quantification of the SARS-CoV-2 virus with an ultrasensitive nanobody-based photonic nanosensor

Author(s): Gisela Ruiz-Vega; Maria Soler; M. Carmen Estevez; Patricia Ramirez-Priego; Martalu D. Pazos; María A. Noriega; Yago Margolles; Clara Francés-Gómez; Ron Geller; Giulia Matusali; Francesca Colavita; Antonino di Caro; José M. Casasnovas; Luis Angel Fernández; Laura M. Lechuga
Published in: Sensors and Diagnostics, 1, 2022, ISSN 2635-0998
Publisher: Royal Society of Chemistry
DOI: 10.1039/d2sd00082b

On Behalf Of Inmi ReCOVeRI Study Group. Frequency and Duration of SARS-CoV-2 Shedding in Oral Fluid Samples Assessed by a Modified Commercial Rapid Molecular Assay

Author(s): Bordi L, Sberna G, Lalle E, Piselli P, Colavita F, Nicastri E, Antinori A, Boumis E, Petrosillo N, Marchioni L, Minnucci G, D'Agostini E, Castilletti C, Locatelli F, Zumla A, Ippolito G, Capobianchi MR
Published in: Viruses, 19994915, 2020, ISSN 1999-4915
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/v12101184

How Nanophotonic Label-Free Biosensors Can Contribute to Rapid and Massive Diagnostics of Respiratory Virus Infections: COVID-19 Case

Author(s): Maria Soler, Maria Carmen Estevez, Maria Cardenosa-Rubio, Alejandro Astua, Laura M. Lechuga
Published in: ACS Sensors, 5/9, 2020, Page(s) 2663-2678, ISSN 2379-3694
Publisher: ACS Publications
DOI: 10.1021/acssensors.0c01180

SARS-CoV-2 Phylogenetic Analysis, Lazio Region, Italy

Author(s): Bartolini B, Rueca M, Gruber CEM, Messina F, Carletti F, Giombini E, Lalle E, Bordi L, Matusali G, Colavita F, Castilletti C, Vairo F, Ippolito G, Capobianchi MR, Di Caro A.
Published in: Emerg Ingectious diseases, 10806059, 2020, ISSN 1080-6059
Publisher: Centers for disease control and prevention
DOI: 10.3201/eid2608.201525

Label-Free Plasmonic Biosensor for Rapid, Quantitative, and Highly Sensitive COVID-19 Serology: Implementation and Clinical Validation

Author(s): Calvo-Lozano O, Sierra M, Soler M, Estévez MC, Chiscano-Camón L, Ruiz-Sanmartin A, Ruiz-Rodriguez JC, Ferrer R, González-López JJ, Esperalba J, Fernández-Naval C, Bueno L, López-Aladid R, Torres A, Fernández-Barat L, Attoumani S, Charrel R, Coutard B, Lechuga LM
Published in: analytical chemistry, 15206882, 2022, ISSN 1520-6882
Publisher: ACS Publications
DOI: 10.1021/acs.analchem.1c03850

16S rRNA gene sequencing of rectal swab in patients affected by COVID-19

Author(s): Mazzarelli A, Giancola ML, Farina A, Marchioni L, Rueca M, Gruber CEM, Bartolini B, Ascoli Bartoli T, Maffongelli G, Capobianchi MR, Ippolito G, Di Caro A, Nicastri E, Pazienza V
Published in: PLoS One, 19326203, 2021, ISSN 1932-6203
Publisher: Public Library of Science
DOI: 10.1371/journal.pone.0247041