Periodic Reporting for period 1 - FPNP-BioLED (Fluorescent Protein-metal oxide NanoParticles for Bio-hybrid Light-Emitting Diodes)
Reporting period: 2021-09-01 to 2023-08-31
The project aimed to enhance the stability of fluorescent proteins (FPs) within Bio-High-Efficiency Lighting Emitting Diodes (Bio-HLEDs) by developing hybrid nanomaterials based on FPs encapsulated in silica (FP@SiO2/MOx). Unfortunately, the goal of creating a double-shell structure wasn't achieved due to the inherent instability of the proteins. Consequently, our research shifted its focus toward the development of SiO2 hybrid materials.
Our primary objectives were to pioneer innovative techniques for encapsulating FPs within silica nanoparticles (FP@SiO2) to enhance their stability and optical properties.
We devised two innovative approaches: one involved the supramolecular modification of carboxylic acid groups, while the other utilized silyl ester functionalization under physiological conditions. These methods resulted in the creation of highly stable monochromatic and dual-emissive FP@SiO2 nanoparticles.These nanoparticles demonstrated exceptional stability and optical properties, even under challenging conditions and in organic solvents. In Bio-HLEDs, coatings with FP@SiO2 achieved complete conversion with high color purity and demonstrated remarkable stability under high-power conditions.
Our primary objectives were to pioneer innovative techniques for encapsulating FPs within silica nanoparticles (FP@SiO2) to enhance their stability and optical properties.
We devised two innovative approaches: one involved the supramolecular modification of carboxylic acid groups, while the other utilized silyl ester functionalization under physiological conditions. These methods resulted in the creation of highly stable monochromatic and dual-emissive FP@SiO2 nanoparticles.These nanoparticles demonstrated exceptional stability and optical properties, even under challenging conditions and in organic solvents. In Bio-HLEDs, coatings with FP@SiO2 achieved complete conversion with high color purity and demonstrated remarkable stability under high-power conditions.
From the project's initiation to the conclusion of the reporting period, substantial work has been conducted in alignment with the project's objectives and milestones. The project aimed to address several key objectives:
Objective 1: The primary goal was to establish innovative synthesis protocols for white FP@SiO2/MOx nanoparticles. While the achievement of the double-shell structure was not realized due to the limited stability of some proteins and more time consuming synthetic part, the project made remarkable progress in this area by introducing two groundbreaking methods for SiO2 encapsulation. One method involved the supramolecular modification of carboxylic acid groups, while the other was based on physiological conditions. These methods proved to be exceptionally successful, enabling the development of highly stable white FP@SiO2 nanoparticles that retained their emissive properties even under challenging conditions and in organic solvents.
Objective 2: Another crucial objective was to develop a novel method for enhancing the stability of fluorescent proteins (FP) under challenging device operating conditions. The project successfully realized this objective, resulting in the creation of highly stable monochromatic and dual-emissive FP@SiO2 nanoparticles.
Objective 3: In addition to the nanoparticle work, the project aimed to create single-layered and single-component white color filters for highly efficient Bio-High Luminance Emitting Diodes (Bio-HLEDs). While this objective was a secondary focus, it was aligned with our overarching goal of advancing optoelectronics.
The results achieved in the project are substantial and hold significant promise for various applications:
i) The successful implementation of the shell SiO2 approach led to the creation of highly stable monochromatic and dual-emissive FP@SiO2 nanoparticles. These nanoparticles exhibited exceptional emissive properties even under demanding conditions and in organic solvents. The introduction of the two innovative synthetic methods for white FP@SiO2 nanoparticles marked a significant advancement in the field. These nanoparticles have the potential to revolutionize optoelectronics by offering stable and efficient white emission.
ii) The development of single-layered and single-component white color filters for Bio-HLEDs holds promise for achieving high color purity and luminous efficacy in lighting applications.
The dissemination aspect has been extensively described in the technical report.
Objective 1: The primary goal was to establish innovative synthesis protocols for white FP@SiO2/MOx nanoparticles. While the achievement of the double-shell structure was not realized due to the limited stability of some proteins and more time consuming synthetic part, the project made remarkable progress in this area by introducing two groundbreaking methods for SiO2 encapsulation. One method involved the supramolecular modification of carboxylic acid groups, while the other was based on physiological conditions. These methods proved to be exceptionally successful, enabling the development of highly stable white FP@SiO2 nanoparticles that retained their emissive properties even under challenging conditions and in organic solvents.
Objective 2: Another crucial objective was to develop a novel method for enhancing the stability of fluorescent proteins (FP) under challenging device operating conditions. The project successfully realized this objective, resulting in the creation of highly stable monochromatic and dual-emissive FP@SiO2 nanoparticles.
Objective 3: In addition to the nanoparticle work, the project aimed to create single-layered and single-component white color filters for highly efficient Bio-High Luminance Emitting Diodes (Bio-HLEDs). While this objective was a secondary focus, it was aligned with our overarching goal of advancing optoelectronics.
The results achieved in the project are substantial and hold significant promise for various applications:
i) The successful implementation of the shell SiO2 approach led to the creation of highly stable monochromatic and dual-emissive FP@SiO2 nanoparticles. These nanoparticles exhibited exceptional emissive properties even under demanding conditions and in organic solvents. The introduction of the two innovative synthetic methods for white FP@SiO2 nanoparticles marked a significant advancement in the field. These nanoparticles have the potential to revolutionize optoelectronics by offering stable and efficient white emission.
ii) The development of single-layered and single-component white color filters for Bio-HLEDs holds promise for achieving high color purity and luminous efficacy in lighting applications.
The dissemination aspect has been extensively described in the technical report.
The project has made remarkable progress beyond the state of the art in several ways:
Synthetic Protocols for White FP@SiO2 Nanoparticles: The introduction of two innovative synthetic methods for white FP@SiO2 nanoparticles is a groundbreaking achievement. These nanoparticles offer stable and efficient white emission, a feat that was previously challenging to achieve. This progress holds the potential to revolutionize optoelectronics, enabling the development of highly efficient and stable white light-emitting devices.
Stability of Fluorescent Proteins (FPs): The project achieved a significant breakthrough by enhancing the stability of FPs under challenging device operating conditions. The development of the SiO2 shell approach represents a pioneering advancement. This novel method provides a level of stability for FPs that surpasses current industry standards, ensuring that these proteins retain their exceptional emissive properties even under harsh conditions and in organic solvents.
Development of High-Performance Bio-HLEDs: Building upon our achievements, our goal is to create high-performance Bio-High Luminance Emitting Diodes (Bio-HLEDs) that utilize the stable and efficient FP@SiO2 nanoparticles. We will work on achieving high color purity, luminous efficacy, and device stability.
The development of stable and efficient FP@SiO2 nanoparticles and their application in lighting technologies can have profound socio-economic and social impacts. These innovations may lead to the production of more energy-efficient and durable lighting devices, reducing energy consumption and associated costs for both consumers and businesses. This, in turn, can contribute to a more sustainable and environmentally friendly future, with reduced carbon emissions and energy waste.
Industrial Applications: The project's outcomes offer opportunities for collaboration with industries involved in lighting, optoelectronics, and materials science. While progress has been made, it's important to acknowledge that further enhancements are required to make these innovations commercially competitive and attractive to industrial partners. The potential to create commercial products with improved performance and stability could drive economic growth in these sectors, benefiting both industry and society at large.
Social Impact: Beyond the economic benefits, the project's innovations can have a positive social impact by improving access to energy-efficient lighting solutions. This can benefit communities, especially in regions with limited access to reliable electricity, by providing them with more sustainable and affordable lighting options. Additionally, reduced energy consumption can contribute to lower energy bills for households, alleviating financial burdens and improving overall quality of life.
Synthetic Protocols for White FP@SiO2 Nanoparticles: The introduction of two innovative synthetic methods for white FP@SiO2 nanoparticles is a groundbreaking achievement. These nanoparticles offer stable and efficient white emission, a feat that was previously challenging to achieve. This progress holds the potential to revolutionize optoelectronics, enabling the development of highly efficient and stable white light-emitting devices.
Stability of Fluorescent Proteins (FPs): The project achieved a significant breakthrough by enhancing the stability of FPs under challenging device operating conditions. The development of the SiO2 shell approach represents a pioneering advancement. This novel method provides a level of stability for FPs that surpasses current industry standards, ensuring that these proteins retain their exceptional emissive properties even under harsh conditions and in organic solvents.
Development of High-Performance Bio-HLEDs: Building upon our achievements, our goal is to create high-performance Bio-High Luminance Emitting Diodes (Bio-HLEDs) that utilize the stable and efficient FP@SiO2 nanoparticles. We will work on achieving high color purity, luminous efficacy, and device stability.
The development of stable and efficient FP@SiO2 nanoparticles and their application in lighting technologies can have profound socio-economic and social impacts. These innovations may lead to the production of more energy-efficient and durable lighting devices, reducing energy consumption and associated costs for both consumers and businesses. This, in turn, can contribute to a more sustainable and environmentally friendly future, with reduced carbon emissions and energy waste.
Industrial Applications: The project's outcomes offer opportunities for collaboration with industries involved in lighting, optoelectronics, and materials science. While progress has been made, it's important to acknowledge that further enhancements are required to make these innovations commercially competitive and attractive to industrial partners. The potential to create commercial products with improved performance and stability could drive economic growth in these sectors, benefiting both industry and society at large.
Social Impact: Beyond the economic benefits, the project's innovations can have a positive social impact by improving access to energy-efficient lighting solutions. This can benefit communities, especially in regions with limited access to reliable electricity, by providing them with more sustainable and affordable lighting options. Additionally, reduced energy consumption can contribute to lower energy bills for households, alleviating financial burdens and improving overall quality of life.