Tailoring the electrode-enzyme interface for efficient bioelectrochemical CO2 reduction by formate dehydrogenase

Project Information

BERCO2

Grant agreement ID: 101024443

DOI

10.3030/101024443

Project closed

EC signature date 11 March 2021

Start date 1 September 2021

End date 31 August 2023

Funded under

• EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions

Total cost

€ 191 149,44

EU contribution

€ 191 149,44

Coordinated by

UNIVERSITE DE GENEVE
Switzerland

Periodic Reporting for period 1 - BERCO2 (Tailoring the electrode-enzyme interface for efficient bioelectrochemical CO2 reduction by formate dehydrogenase)

Reporting period: 2021-09-01 to 2023-08-31

Summary of the context and overall objectives of the project

The "BERCO2" project, funded under the Marie Skłodowska-Curie Action (MSCA), addresses the urgent global challenge of mitigating climate change by developing efficient and sustainable methods for carbon dioxide (CO2) reduction. Specifically, the project focuses on optimizing the electrochemical reduction of CO2 using enzymes, with a primary focus on formate dehydrogenase (FDH H).
As the world grapples with the consequences of excessive CO2 emissions, finding effective ways to reduce atmospheric CO2 levels is paramount. The "BERCO2" project holds the promise of contributing to a sustainable future by harnessing the power of enzymes to convert CO2 into useful chemicals. This technology can potentially enable the production of sustainable fuels and chemicals, reducing our reliance on fossil fuels and mitigating climate change. Furthermore, it aligns with European policy objectives and strategies for environmental sustainability and innovation.
The overall aim of the project is to develop efficient bioelectrochemical methods for reducing CO2 emissions, with a focus on optimizing the interface between enzymes and electrodes, ultimately contributing to sustainable CO2 reduction.

Work performed from the beginning of the project to the end of the period covered by the report and main results achieved so far

The "BERCO2" project has been diligently pursued with a commitment to advancing sustainable CO2 reduction through bioelectrochemistry. While we encountered certain challenges and deviations from our original plan, our dedication to our primary goal remained unwavering.

Formylmethanofuran dehydrogenase (Fmd), capable of catalyzing the reduction of CO2. This enzyme plays a crucial role in the initial step of methanogenesis. In this project we used Fmd enzyme purified from the thermophilic methanogen Methermicoccus shengliensis.

In the subsequent phase of our research, we explored the electrochemical behavior of Fmd when adsorbed onto electrodes, particularly graphite rod electrodes (GRE). Through amperometric i-t curve analysis, we gained valuable insights into the enzyme's performance and its ability to catalyze electrochemical reactions. We also determined specific enzyme properties, such as the Michaelis-Menten constant (Km) and the maximum reaction velocity (Vmax), for both CO2 reduction and formate oxidation.

In summary, despite challenges and strategic deviations, our project has made substantial progress toward achieving its overarching goal of advancing sustainable CO2 reduction through bioelectrochemistry. The outcomes of our research hold the potential to benefit society by offering innovative solutions to address climate change and environmental sustainability.

Progress beyond the state of the art and expected potential impact (including the socio-economic impact and the wider societal implications of the project so far)

One notable aspect of the BERCO2 project was the exploration of formylmethanofuran dehydrogenase (Fmd) as an alternative enzyme for CO2 reduction. This research led us to explore the electrochemical behavior of Fmd when adsorbed onto electrodes, providing valuable insights into its performance. Our results and methodologies contribute to expanding the understanding of huge complex metalloenzyme behavior in bioelectrochemical systems.
As we look ahead to the final phase of the project, our objectives remain clear. We aim to further optimize the electrochemical processes involving Fmd and electrodes, with a focus on enhancing catalytic efficiency. This includes exploring different electrode materials and experimental conditions to maximize CO2 reduction rates. Additionally, we plan to continue our efforts in demonstrating bioelectrosynthesis, converting CO2 into formate under reductive potential.
The outcomes of our research hold significant potential for both socio-economic and wider societal impacts. By advancing the understanding of bioelectrochemical CO2 reduction, we contribute to the development of sustainable technologies for addressing climate change. Our work has implications for the creation of innovative solutions in renewable energy and carbon capture, with the potential to shape future industrial processes. Furthermore, our project fosters international collaboration and knowledge exchange, strengthening research networks and promoting scientific cooperation. Overall, our commitment to advancing sustainable CO2 reduction through bioelectrochemistry aligns with global goals for environmental stewardship and offers promise for a more sustainable future.

Site-specifically attached FDH H on electrode surface