Project description
Lytic polysaccharide monooxygenases regulation through Ascomycota enzymes
Lytic polysaccharide monooxygenases (LPMOs) are oxidative enzymes used by fungi to depolymerise cellulose and are of great interest for their application in biorefineries. Recent studies indicate that LPMOs benefit from two oxidative enzymes (i.e. polyphenol oxidases and laccases) that are active on (poly)phenolic compounds. The EU-funded FuelingLPMO project will investigate if and how a novel group of uncharacterised flavoenzymes, commonly co-secreted with AA9 LPMOs by ascomycetes during lignocellulose degradation, can regulate LPMO activity by modifying lignin-derived compounds. Results will contribute to a more detailed understanding of the network of redox reactions involved in lignocellulose degradation by ascomycetes and help design powerful enzyme systems for efficient biomass conversion into bioproducts.
Objective
Lytic polysaccharide monooxygenases (LPMOs) are oxidative enzymes employed by fungi to depolymerize cellulose and thus of great academic and industrial (e.g. biorefinery) interest. The exact way or ways natural electron and H2O2 supply occurs to LPMOs during natural fungal LPMO action (and activation) remains unclear. Recent evidence suggests that LPMOs benefit from two oxidative enzymes (i.e. polyphenol oxidases (PPOs) and laccases) that are active on (poly)phenolic compounds. This gives rise to a new paradigm that places lignin as a protagonist in the activation of cellulose degradation, and not simply an obstacle to biomass decay. FuelingLPMO aims to investigate if and how a novel group of uncharacterized flavoenzymes, which I and other researchers have independenlty reported to be commonly co-secreted with AA9 LPMOs by ascomycetes during lignocellulose degradation, can regulate LPMO activity by modifying lignin-derived compounds, as well as deepen the current understanding on the mechanisms involved in LPMO activation by PPOs and laccases. We will use a combination of genome database mining, proteomics analysis and enzyme synergy characterization to investigate the co-occurence of genes encoding for AA9 LPMOs and these putative LPMO regulatory enzymes among Ascomycota, validate their co-secretion during lignocellulose degradation and test their hypothesized role in the regulation of cellulose degradation by AA9 LPMOs in vitro. The project will form a collaboration between the two research laboratories that made the ground-breaking discoveries of the PPO-mediated and laccase-mediated activation of LPMOs and exploit the multi-/interdisciplinary nature of their studies. Our findings will contribute to a more detailed understanding of the network of redox reactions involved in lignocellulose degradation by ascomycetes, building on the hypothesis that such understanding can be used to design powerful enzyme systems for efficient biomass conversion into bioproducts.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins proteomics
- natural sciences chemical sciences organic chemistry organic reactions
- natural sciences biological sciences microbiology mycology
- natural sciences biological sciences biochemistry biomolecules carbohydrates
- natural sciences biological sciences biochemistry biomolecules proteins enzymes
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions
MAIN PROGRAMME
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H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility
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Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF)
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Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) H2020-MSCA-IF-2020
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Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
2800 KONGENS LYNGBY
Denmark
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.