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Resolving the molecular basis underlying convergent evolution of DODA enzymes in the betalain synthesis pathway

Project description

Studies into the betalain synthesis pathway elucidate the evolution of molecular complexity

Studying convergent evolution can help scientists better understand the genetic basis of the repeated emergence and optimisation of a metabolic pathway. The central enzyme in the betalain pathway, 4,5-DOPA dioxygenase extradiol (DODA), for example, has evolved four times via gene duplication and neofunctionalisation. The EU-funded ConvergentDODA project will explain the molecular basis underlying the repeated evolution of L-DOPA dioxygenase activity and determine the molecular evolutionary trajectories behind different enzymatic catalytic profiles across different origins of betalain synthesis. By investigating the molecular basis of the convergent evolution of high DODA activity, the project will address key questions in evolutionary biology such as the respective roles of parallel evolution, epistasis, adaptive constraint and contingency.

Objective

As sessile organisms, plants have evolved a diverse array of specialized metabolites to respond to their environments. Specialised metabolites are frequently restricted to particular plant lineages, such as the specialised betalain pigments, which are unique to the flowering plant order Caryophyllales - betalains will be familiar as the colour of beetroot. However there are also striking examples of convergent evolution, with the same specialized metabolites emerging independently in different lineages. The host laboratory has recently shown that the betalain biosynthesis pathway has evolved multiple times within Caryophyllales. Underpinning four convergent origins of betalain pigmentation, the central enzyme in the betalain pathway, 4,5-DOPA-extradiol -dioxygenase (DODA), has evolved four times via gene duplication and neofunctionalization. Preliminary data indicate that DODA enzymes from different origins have distinct kinetic profiles, of significance to biotechnology. In the proposed research, I will elucidate the molecular basis underlying the repeated evolution of L-DOPA dioxygenase activity, and resolve the molecular evolutionary trajectories underlying different enzymatic catalytic profiles across different origins of betalain synthesis. Convergent evolution provides a natural experiment in understanding the genetic basis of repeated emergence and optimization of a metabolic pathway. In exploring the molecular basis of the convergent evolution of high DODA activity, I expect to address many central questions in evolutionary biology, including the respective roles of parallel evolution, epistasis, adaptive constraint, and contingency, in the evolution of molecular complexity.

Coordinator

THE CHANCELLOR MASTERS AND SCHOLARS OF THE UNIVERSITY OF CAMBRIDGE
Net EU contribution
€ 212 933,76
Address
TRINITY LANE THE OLD SCHOOLS
CB2 1TN Cambridge
United Kingdom

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Region
East of England East Anglia Cambridgeshire CC
Activity type
Higher or Secondary Education Establishments
Links
Total cost
€ 212 933,76