Project description
Functional introduction of newborn neurons into existing brain neural circuits
New cells must integrate into the network of existing cells for embryonic growth and regeneration to take place. The EU-funded Imagine project aims to use the brain of the axolotl (Ambystoma mexicanum) to study the dynamic remodelling of neural circuits when introducing newborn neurons in growth and regeneration stages. The researchers will employ a high-resolution intra-vital in vivo imaging approach to investigate the dynamics of neurogenesis and remodelling of neural circuits in post-embryonic growth and regeneration and develop a visualisation of the functional neuronal connections in the axolotl. Combining the imaging set-up with genetically encoded calcium indicators will result in the creation of activity maps of brain regions in the presence of defined stimuli.
Objective
Post embryonic growth and regeneration require new cells to integrate into the network of existing cells. In the case of the brain it is extremely important to maintain and restore circuit function, such that behaviors can be executed correctly. To study such processes a system that fulfills both post-embryonic growth as well as regeneration is needed. Furthermore, since neural circuit functions are highly dynamic the need for in vivo approaches is evident. In mammalian systems where neurogenesis occurs throughout life in restricted brain regions such approaches are technically challenging.
Here, I propose to use the axolotl (Ambystoma mexicanum) brain to understand the dynamic remodeling of neural circuits through the addition of newborn neurons during post-embryonic growth and regeneration. I will establish a high resolution intra-vital in vivo imaging approach to address the dynamics of neurogenesis and remodeling of neural circuits in the telencephalon and the optic tectum. Using this setup in combination with genetically encoded calcium indicators I will generate activity maps of these regions in the presence or absence of defined stimuli and address their functional remodeling during growth and their restoration after injury. Furthermore, I will develop and adapt methods to visualize the functional connections of neurons in axolotl. Together, this work will provide fundamental insights into the functional maintenance and regeneration of neural circuits and the dynamics of how new neurons integrate in vivo which has not yet been achieved in any vertebrate system.
Fields of science (EuroSciVoc)
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Programme(s)
Funding Scheme
MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF)Coordinator
1030 Wien
Austria