Project description
Innovation for nitrogen-fixing bacteria detection
Plant growth-promoting rhizobacteria (PGPR) provide necessary nutrients to plants, potentially substituting chemical fertilisers. Especially their ability to fix nitrogen is important. Several techniques to screen nitrogen-fixing bacteria are tedious and inefficient. A rapid, cost-effective membrane-based sensor emerges as an alternative. Interestingly, in extreme saline and alkaline lime environments, there are diverse microbial communities. Isolating PGPR from these environments could help mitigate salinity stress in different crops such as wheat. The EU-funded NitroFixSal project will develop an innovative membrane-based sensor for nitrogen-fixing bacteria detection and isolation of PGPR from extreme environments. The project will also study suppression subtractive hybridisation to understand plant-PGPR interaction.
Objective
Plant Growth-Promoting Rhizobacteria (PGPR) provide necessary nutrients to the plants and are promising substitute for the chemical fertilizers to promote plant growth and yield. Among various growth promotion properties of PGPR, the ability to fix N2 is important for plant growth. Several media-based techniques are available to screen the N2 fixing bacteria that are tedious, time-consuming and requires significant amount of resources. Therefore, a rapid, cost-effective membrane-based sensor can be a good alternative of these media-based screening methods. Further, a reservoir of diverse microbial communities is present in a unique extreme environment - saline and alkaline lime in Janikowo, Poland. Isolating PGPR from such extreme environments can be useful for mitigating salinity stress on different crops e.g. wheat (Triticum aestivum), which is one of the most important crops in the world facing significant yield loss in the production due to soil salinity. Also, the study of expression of genes that are differentially expressed in wheat upon interaction with PGPR can result in a better understanding of plant-microbe interaction. Hence, the work is proposed in a sequential manner where the membrane-sensor will be prepared to screen N2 fixing bacteria from the samples collected from extreme environments and allowed to interact with wheat plant under saline condition to check its growth promotion effects. Then the most effective strains/consortia for growth promotion will be selected. Finally, Suppression Subtractive Hybridization (SSH) will be performed to study differentially expressed genes in wheat plants upon interaction with selected strains/consortia. The project is expected to develop innovative membrane-based sensor for the detection of N2 fixing bacteria and isolation of novel and potential halotolerant PGPR from anthropogenic extreme environments. SSH based gene profiling study will also be a new approach to understand plant-PGPR interaction.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences microbiology bacteriology
- natural sciences biological sciences microbiology microbiomes
- engineering and technology electrical engineering, electronic engineering, information engineering electronic engineering sensors
- agricultural sciences agriculture, forestry, and fisheries agriculture grains and oilseeds cereals
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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H2020-EU.4. - SPREADING EXCELLENCE AND WIDENING PARTICIPATION
MAIN PROGRAMME
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Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF)
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Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) H2020-WF-2018-2020
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Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
87-100 TORUN
Poland
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.