Project description
Advanced organoid engineering techniques to boost disease and drug development research
Cerebral organoids are 3D self-assembled structures derived from human induced pluripotent stem cells, replicating both the structure and function of the human foetal brain. Organoids could replace existing 2D in vivo cell cultures and animal models for diagnostic and treatment purposes, but this has not yet been realised owing to a lack of suitable preparation methods. The EU-funded PHOENIX project aims to develop a seamless organoid engineering pipeline to investigate reproducibility, controlled maturation and vascularisation of cerebral organoids. Using acoustophoresis, a method of manipulating particles and cells by ultrasound, researchers will seek to achieve ordered encapsulation of stem cells in hydrogel droplets. They will also develop a microfluidic platform where the cells will be differentiated under fully controlled conditions.
Objective
The aim of PHOENIX is to use my expertise in microsystems engineering to close critical technology gaps in organoid generation. Cerebral organoids are 3D self-assembled structures derived from human induced pluripotent stem cells, replicating both structure and function of the human foetal brain. Organoids have the potential to replace existing 2D cell cultures and animal models, but this has not yet been realised due to rudimentary preparation methods.
In PHOENIX, three important technology gaps will be addressed: reproducibility, controlled maturation and vascularisation. I aim to build on my pioneering research on droplet acoustofluidics and the scientific output of my ERC Starting Grant to develop three microfluidic modules that at the end of the project shall be integrated into a seamless organoid engineering pipeline. The technology in focus is acoustophoresis, a method to manipulate particles and cells by ultrasound. This will be used to achieve ordered encapsulation of stem cells in hydrogel droplets and develop a microfluidic platform where the cells can be differentiated under fully controlled conditions. Finally, two-photon writing will be used to integrate a vascular network with the organoid constructs to form an important delivery architecture for nutrients and blood components. PHOENIX will be focused on both technology development and thorough biological characterisation of the resulting organoids to demonstrate both expected, and unexpected, benefits of transferring organoid generation on-chip.
Collaborations have been established with Prof. Christine Mummery and Dr. Valeria Orlova, both at LUMC, NL as well as Dr. Anna Falk at KI, SE to provide expertise in complementary fields of this highly interdisciplinary project. The expected output of PHOENIX is a microfluidic technology that enables high-throughput generation of cerebral organoid with a multi-regional structure and vascularisation in a direct process.
Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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HORIZON.1.1 - European Research Council (ERC)
MAIN PROGRAMME
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Topic(s)
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Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
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Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
HORIZON-ERC - HORIZON ERC Grants
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Call for proposal
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) ERC-2021-COG
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Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
751 05 Uppsala
Sweden
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