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Towards increased biosafety for non-target insects - Damage-activated proteolysis to selectively enhance toxicity of pesticides

Project description

Innovative method to improve pesticide selectivity

Pesticides, particularly the toxic effects of insecticides on non-target insects such as bees, are among the main factors behind the rapid decline of insect numbers and diversity worldwide. The EU-funded DETOXPEST project proposes an innovative approach for the damage activation of pro-pesticides by plant proteases activated in the gut of feeding caterpillars. The method does not hurt non-target beneficial insects that cause no damage, including pollinators and natural enemies of pests. In this context, DETOXPEST will study the impact of fall armyworm (Spodoptera frugiperda), an invasive insect pest and a potential major threat to EU agriculture, on the model plant Arabidopsis thaliana and the crop maize.

Objective

Insects numbers and diversity have rapidly declined in the EU and worldwide. Pesticides, and particularly the toxicity of insecticides to non-target insects (e.g. bees), are one of the major drivers of insect declines. Apart from destabilizing natural ecosystems, pollinator disappearance directly threatens food security.
To help combat insect decline, I propose an innovative approach for the damage-activation of pro-pesticides (DAPP) by plant proteases that are activated in the gut of feeding caterpillars. Non-target beneficial insects that cause no damage, including pollinators and natural enemies of pests, are spared.
My team pioneers the study of proteolysis in the plant wound response, following our recent discovery that physical damage activates a class of proteases, called metacaspases. I hypothesize that i) damage-activated plant proteolysis is a largely unrecognized but potential key player in the plant wound response to insect herbivores and ii) this knowledge can be used to enhance pesticide biosafety.
We will study the impact of fall armyworm (Spodoptera frugiperda), an invasive insect pest and potential major threat to EU agriculture, on the model plant Arabidopsis thaliana and the economically important crop maize. A combination of advanced (N-terminomics) and novel (Proteome Integral Solubility Alteration) proteomics technologies will allow us to uncover unknown plant metacaspase substrates and damage-activated proteases and to assess their impact on insect herbivory. These fundamental studies will feed information into a pipeline of first-in-class DAPP development, where we will modify biological insecticides with newly-discovered protease cleavage sites. Finally, we will test toxicity against target (Spodoptera) and non-target insects.
My early-stage and fundamental research on damage-activated proteolysis can have a tremendous positive impact on the increase of insecticide selectivity to help combat the escalating problem of insect decline.

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Topic(s)

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HORIZON-ERC - HORIZON ERC Grants

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Call for proposal

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(opens in new window) ERC-2021-COG

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Host institution

SVERIGES LANTBRUKSUNIVERSITET
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 1 999 720,00
Address
ALMAS ALLE 8
750 07 Uppsala
Sweden

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Region
Östra Sverige Östra Mellansverige Uppsala län
Activity type
Higher or Secondary Education Establishments
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

€ 1 999 720,00

Beneficiaries (1)

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