Objective
G protein-coupled receptor (GPCR) are membrane proteins targeted by around 30 % of all marketed drugs. GPCR internalization is a mechanism that tunes the cell surface versus intracellular receptor ratio, a parameter that plays an essentiG protein-coupled receptor (GPCR) internalization is a mechanism involved in the tuning of cell surface versus intracellular receptor ratio, a parameter that plays an essential role in controlling receptor physiological responses. This process is very well described for many GPCRs but surprisingly not for metabotropic glutamate receptors, mandatory dimeric GPCRs that are ubiquitously expressed in the brain to regulate neuronal transmission. Deciphering which are the internalization pathways related to mGlus emerge as a strategy for the regulation of their response to not only drugs but also endogenous neurotransmitters.
I propose to examine the internalization profiles of mGlus in brain cells and to unravel the structural and molecular mechanisms involved in the process. I will use a biophysical technique optimized by the host team that allows the real-time and quantitative measurement of receptor internalization in both recombinant cells and brain cells, in combination with a microscopy approach that will complement the analysis. On top of that, I will use nanomolar range affinity nanobodies, single domain antibodies that have already been developed by the host team as a tool for ultimately elucidate which are the internalization profile of mGlus in brain cells.
As a consequence of this project, the internalization profile of mGlus in neurons and astrocytes, and the cell machinery responsible for this process will be clarified for the first time. This knowledge will allow the fine-tuning of mGlus localization in the cell, as well as the validation of the nanobodies as a tool for studying endogenous mGlus. These outcomes will upgrade the current resources that the scientific community has to modulate mGlu receptor-mediated responses.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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HORIZON.1.2 - Marie Skłodowska-Curie Actions (MSCA)
MAIN PROGRAMME
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Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
HORIZON-TMA-MSCA-PF-EF - HORIZON TMA MSCA Postdoctoral Fellowships - European Fellowships
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Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) HORIZON-MSCA-2021-PF-01
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Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
75794 PARIS
France
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.