Objective
Neurodegenerative disorders are characterised by the progressive deterioration and loss of certain neuronal populations. Distinct clinical features are associated with different diseases, but common fundamental processes have been identified. One example of this is injury via oxidative stress. The production of oxidative species is linked to mitochondrial dysfunction and inflammation, which have been demonstrated to occur early in these disorders. Identifying the proteins that are involved in the response to these species is of critical importance to understanding these diseases. This will provide opportunities to identify novel biomarkers and develop treatments that are effective at these early stages.
The use of genetic code expansion to functionalise proteins with clickable amino acids is an emerging protein engineering technology with many potential applications in neurobiology. Recently, it has been demonstrated that any of the 20 natural amino acids can be replaced with a non-canonical amino acid (ncAA) on a proteome-wide scale. However, the application of this technology in complex cells, such as neurons, is still in its infancy. I aim to establish a novel proteome-wide ncAA incorporation methodology to monitor changes in the neuronal proteome in response to oxidative injury. This will be achieved using ncAAs that have been optimised for an inverse-electron-demand Diels-Alder click reaction, which is highly biorthogonal and has extremely rapid kinetics. Adding a clickable biotin handle will facilitate protein enrichment in a highly time-resolved manner. Subsequent proteomic analysis will be applied to identify changes within the whole proteome or subsets of the proteome. Monitoring changes in protein expression in this way will provide valuable information about neurodegenerative disorders and allow for precise investigations of potential new drug targets and biomarkers.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences neurobiology
- natural sciences biological sciences biochemistry biomolecules proteins proteomics
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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HORIZON.1.2 - Marie Skłodowska-Curie Actions (MSCA)
MAIN PROGRAMME
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Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
HORIZON-TMA-MSCA-PF-EF - HORIZON TMA MSCA Postdoctoral Fellowships - European Fellowships
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Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) HORIZON-MSCA-2021-PF-01
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Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
72074 Tuebingen
Germany
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.