Objective
Most microorganisms lack homeothermic regulation which subjects them to unpredictable fluctuations in environmental temperature. Nevertheless, many bacteria and archaea can grow across a large range of up to 40C. Most biochemical reactions in bacteria occur in the highly crowded cytoplasm constituting a complex and dynamic interaction network of a cell. Temperature affects the rate of both intra- and intermolecular reactions, and large-scale perturbations by temperature could be disastrous to cellular function, homeostasis, and sub-cellular organization. It is still largely unknown, how temperature affects the properties of the cytoplasm and what the consequences to cellular processes are. The driving hypothesis of this project is that bacteria can actively modulate their cytoplasmic state to avoid the detrimental effects of temperature fluctuations.
To test this, I have established cutting-edge super-resolution single-molecule tracking tools to directly observe molecule dynamics in live bacteria in real time. First, we will quantify the diffusion and activity of macromolecules and other probes as a function of temperature to uncover the changes in the cytoplasmic state. Second, we will probe different bacteria growing at temperatures from 0C up to 100C to characterize evolutionary differences in the cytoplasmic dynamics and temperature scaling of reaction rates. Finally, we will uncover mechanisms by which bacteria obtain different cytoplasmic properties. Overall, these approaches will reveal how the cytoplasmic state in bacteria changes with temperature and how this contributes to the cellular processes, which has significant implications on how microorganisms adapt to temperatures and what are the limits of cellular life.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
- natural sciencesbiological sciencesmicrobiologybacteriology
- natural sciencesphysical sciencesopticsmicroscopysuper resolution microscopy
- medical and health sciencesbasic medicinephysiologyhomeostasis
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Programme(s)
- HORIZON.1.1 - European Research Council (ERC) Main Programme
Topic(s)
Funding Scheme
HORIZON-ERC - HORIZON ERC GrantsHost institution
02150 Espoo
Finland