Project description DEENESFRITPL Animal-free testing of the effects of inhaled nanomaterials Nanotechnology-enabled products are rapidly entering the market and the environment, which necessitates efficient hazard and risk assessments. However, the current slow and costly animal-based testing with limited capacity cannot meet the needs of industry and regulators. Therefore, the EU-funded nanoPASS project will design cost-efficient high-throughput screening approaches for inhalation-related long-term hazards. It proposes a shift of testing focus from late endpoints to early key events leading to adverse outcomes. The early events will be identified by key bridging methods: intravital in vivo microscopy, quantitative time-lapse in vitro microscopies and automated identification of the modes of action. The real-life applicability of developed tests will be demonstrated by performing risk assessment in production and recycling facilities and devising a risk mitigation plan. Show the project objective Hide the project objective Objective The current animal-based testing of materials’ short- and long-term health effects is slow, expensive, and has limited capacity, which stifles the development of new (nano)materials and hinders efficient regulation of the market. To enable cost-efficient high-throughput screening required for industry and regulation, we here propose to shift the focus of nanosafety testing from late endpoints to early key events (KEs) leading to adverse outcomes (AOs). As such tests can only be based on mechanistic understanding, we need to close the knowledge gaps and match KEs in vitro and in vivo, which the nanoPASS consortium is in a unique position to provide. Our key bridging methods are intravital in vivo microscopy, quantitative time-lapse in vitro microscopies, and automated identification of the modes of action (i.e. KE relationships) with proprietary in silico algorithms, supported by datamining of the worlds’ largest in vivo database and single-cell omics data, and computational modelling of structure-function relationships.With this toolset, we aim to 1) develop new in vitro systems that can replicate early KEs leading to AOs related to inhalation of NMs, 2) identify methods to track the dynamics of these KEs, 3) develop quantitative in silico models to predict AOs, and 4) calibrate the in vitro/in silico AO predictions against in vivo data for 40+ well-characterised benchmark materials. Finally, we will 5) validate the AO predictions on several families of industrial materials, sampled from different stages of their life cycle, and then propose reliable testing protocols and guidelines to OECD and ECVAM. With the consortium of 6 complementary research laboratories, SME as technology developer and provider, material producing company as potential end-user, and an industrial association to facilitate dissemination, nanoPASS covers the whole value chain of the new animal-free safety testing technology, and thus paves the way towards safe adoption of new nanotechnologies. Fields of science natural sciencescomputer and information sciencesdatabasesengineering and technologynanotechnologynatural sciencesphysical sciencesopticsmicroscopy Keywords air pollution particulate matter exposure monitoring material production and recycling nanoplastics lung toxicity neurodegeneration molecular initiating events AOP QSAR optical microscopy Programme(s) HORIZON.2.4 - Digital, Industry and Space Main Programme HORIZON.2.4.3 - Emerging enabling technologies Topic(s) HORIZON-CL4-2022-DIGITAL-EMERGING-01-35 - Advanced characterisation methodologies to assess and predict the health and environmental risks of nanomaterials (RIA) Call for proposal HORIZON-CL4-2022-DIGITAL-EMERGING-01 See other projects for this call Funding Scheme HORIZON-RIA - HORIZON Research and Innovation Actions Coordinator INSTITUT JOZEF STEFAN Net EU contribution € 325 300,00 Address Jamova 39 1000 Ljubljana Slovenia See on map Region Slovenija Zahodna Slovenija Osrednjeslovenska Activity type Research Organisations Links Contact the organisation Opens in new window Website Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Total cost € 325 300,00 Participants (8) Sort alphabetically Sort by Net EU contribution Expand all Collapse all INFINITE, RAZVOJ BIO-MEDICINSKIHTEHNOLOGIJ D.O.O. Slovenia Net EU contribution € 486 240,00 Address VRHOVDOLSKA POT 22 2341 LIMBUS See on map Region Slovenija Vzhodna Slovenija Podravska Activity type Private for-profit entities (excluding Higher or Secondary Education Establishments) Links Contact the organisation Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Total cost € 486 240,00 HELMHOLTZ ZENTRUM MUENCHEN DEUTSCHES FORSCHUNGSZENTRUM FUER GESUNDHEIT UND UMWELT GMBH Germany Net EU contribution € 498 873,75 Address INGOLSTADTER LANDSTRASSE 1 85764 Neuherberg See on map Region Bayern Oberbayern München, Landkreis Activity type Research Organisations Links Contact the organisation Opens in new window Website Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Total cost € 498 873,75 DET NATIONALE FORSKNINGSCENTER FOR ARBEJDSMILJØ Denmark Net EU contribution € 501 762,50 Address LERSO PARKALLE 105 2100 Kobenhavn See on map Region Danmark Hovedstaden Byen København Activity type Research Organisations Links Contact the organisation Opens in new window Website Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Total cost € 501 762,50 STATENS ARBEIDSMILJOINSTITUTT Norway Net EU contribution € 452 750,00 Address GYDAS VEI 8 0363 Oslo See on map Region Norge Oslo og Viken Oslo Activity type Research Organisations Links Contact the organisation Opens in new window Website Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Total cost € 452 750,00 LUNDS UNIVERSITET Sweden Net EU contribution € 274 910,00 Address Paradisgatan 5c 22100 Lund See on map Region Södra Sverige Sydsverige Skåne län Activity type Higher or Secondary Education Establishments Links Contact the organisation Opens in new window Website Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Total cost € 274 910,00 UNIVERSITY COLLEGE DUBLIN, NATIONAL UNIVERSITY OF IRELAND, DUBLIN Ireland Net EU contribution € 255 760,00 Address BELFIELD 4 Dublin See on map Region Ireland Eastern and Midland Dublin Activity type Higher or Secondary Education Establishments Links Contact the organisation Opens in new window Website Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Total cost € 255 760,00 NANOTECHNOLOGY INDUSTRIES ASSOCIATION Belgium Net EU contribution € 236 540,00 Address AVENUE DE TERVUEREN 143 1150 Bruxelles / Brussel See on map Region Région de Bruxelles-Capitale/Brussels Hoofdstedelijk Gewest Région de Bruxelles-Capitale/ Brussels Hoofdstedelijk Gewest Arr. de Bruxelles-Capitale/Arr. Brussel-Hoofdstad Activity type Other Links Contact the organisation Opens in new window Website Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Total cost € 236 540,00 ALPACEM CEMENT, DD Slovenia Net EU contribution € 41 600,00 Address ANHOVO 1 5210 Deskle See on map Region Slovenija Zahodna Slovenija Goriška Activity type Private for-profit entities (excluding Higher or Secondary Education Establishments) Links Contact the organisation Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Total cost € 41 600,00