Project description
Revealing how plant cells set the tempo of shoot construction
During plant development, a unique mechanism in the shoot regulates rhythmic organogenesis according to the oscillating distribution of the hormone auxin, where high levels stimulate organ growth. However, unlike traditional theories, the period of shoot organ production (plastochron) cannot solely be determined by periodic auxin oscillations because of noise. The ERC-funded TEMPO project aims to address this knowledge gap in plant biology by identifying how auxin temporal information sustains the tempo of shoot construction. Besides testing whether epigenetic tracking of auxin temporal information establishes a stable plastochron, it will reengineer the plastochron to show that the tempo of shoot construction can be predictively manipulated.
Objective
Time is central to the development of the body plan of multicellular organisms. One prominent developmental timing mechanism is the rhythmic, iterative addition of tissues and organs. While the tempo of rhythmic construction is often set by developmental clocks, plants use a unique mechanism in the shoot, whereby rhythmic organogenesis emerges from dynamic changes in the distribution of the hormone auxin. High auxin levels trigger organogenesis but, contrary to a long-standing theory, the period of shoot organ production - or plastochron - cannot simply be encoded in periodic auxin oscillations, due to noise in these oscillations. Revealing how the tempo of shoot construction is established thus remains a critical knowledge gap in plant biology.
In TEMPO, we hypothesize that cells record and use the history of their auxin exposure, in order to robustly set the timing of organogenesis and the plastochron at the tissue scale despite noisy auxin temporal information. This fundamental change in the way we understand the relationship between auxin and the plastochron stems from preliminary data from my team suggesting histone acetylation as an epigenetic-tracking mechanism, which allows auxin temporal information to be recorded and utilized for transcriptional control.
Uncovering how auxin temporal information establishes the tempo of shoot construction requires multiscale, multidisciplinary approaches. We will combine cutting-edge live-imaging, synthetic biology and computational modeling with innovative optogenetics and single-cell genomics to both ascertain and perturb auxin temporal information and histone acetylation at high resolution, while assessing the effect on cellular transcriptional states and the timing of organ production. Beyond testing whether epigenetic tracking of auxin temporal information sets a robust plastochron across scales, we will reengineer the plastochron to demonstrate that the tempo of shoot construction can be predictively manipulated.
Fields of science (EuroSciVoc)
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CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
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Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
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HORIZON.1.1 - European Research Council (ERC)
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(opens in new window) ERC-2022-ADG
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75794 PARIS
France
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