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The synaptic active zone as a signaling hub for sleep homeostasis and resilience

Project description

Understanding how the synaptic active zone tunes brain resilience

Resilience, the brain's capacity to manage stress, is intricately connected to sleep, and sleep deprivation has been associated with potential neurodegeneration. However, there are knowledge gaps in bridging molecular and cellular aspects with behavioural and organismic levels. The EU-funded SynProtect project investigates the phenomenon of PreScale, i.e. presynaptic active zone plasticity, which is triggered widely in the brain of sleep-deprived Drosophila and enhances resilience to cope with sleep deprivation. The project explores whether PreScale globally remodels presynaptic terminals to adjust resilience states and explore its regulatory role in presynaptic terminals and local excitability via potassium channels. Using advanced imaging and proteomic tools, SynProtect seeks to provide insights into how PreScale contributes to brain resilience by analysing brain activity, signalling and metabolism.

Objective

Resilience designates the ability of the brain to cope with and adapt to stressful situations. Sleep homeostasis is tightly linked to resilience, and the sleep deficits observed alongside neurodegeneration probably operate as direct drivers of neurodegeneration. However, the knowledge gaps still remain huge and causally bridging the molecular/cellular with the behavioral and organismic level remains a challenge, hampering progress equally for biomedical and basic research.
Our recent data suggest that a form of presynaptic active zone plasticity (PreScale), widely triggered in sleep-deprived Drosophila brains, can enhance the brain resilience to cope with the adverse effects of sleep deprivation. Concretely, genetically fostering PreScale in sleepless mutants rescued them from their reduced lifetime, stress sensitivity, cognitive deficits and hyperexcitability due to too low levels of voltage-gated potassium channels.
In SynProtect, we seek to test our hypothesis that PreScale constitutes a globally-operating homeostatic plasticity mechanism remodeling presynaptic terminals comprehensively to tune resilience states.
In order to test this idea, we will elucidate the core molecular scenario executing and bidirectionally regulating PreScale and, consequently, decipher how exactly the remodeling of the mere presynaptic active zones and local excitability tuning via potassium channels intersect at the presynaptic terminal. In parallel, we will test whether PreScale is needed to enhance resilience in a brainwide fashion or if its modus operandi is more local. Genetic manipulation of PreScale will allow us to define brain states of high and
low resilience, which we will dissect combining super-resolution and in vivo activity imaging and proteomic tools. Thus, we will open the way towards a comprehensive insight into the activity, signaling and metabolic profile of brain resilience.

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Call for proposal

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(opens in new window) ERC-2022-ADG

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Host institution

FREIE UNIVERSITAET BERLIN
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 2 242 580,00
Address
KAISERSWERTHER STRASSE 16-18
14195 BERLIN
Germany

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Region
Berlin Berlin Berlin
Activity type
Higher or Secondary Education Establishments
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

€ 2 242 580,00

Beneficiaries (1)

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