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Controlling cellular differentiation of industrial enzyme production in Bacillus subtilis

Objective

To cope with an ever changing environment, bacteria often develop a bet-hedging strategy like bi-stable cellular differentiation, resulting in cell populations with different properties. For example, the industrially relevant model bacterium Bacillus subtilis can become motile, genetically competent, secrete degradative enzymes, or sporulate, and these cellular differentiations can occur simultaneously in a single genetically homogenous population. This cellular heterogeneity exhibited by bacterial cells from the same parent with similar genetic properties is a carefully regulated process that prepares the population for unpredictable environmental stress. Unfortunately, this cellular differentiation can pose serious challenges for the production of enzymes via industrial fermentation, since it means that not all cells in the fermentor are actively involved in enzyme production. This Marie Curie BIOSTAB-DIFF project aims at investigating how enzyme expression differs between individual cells of B. subtilis, and how this cellular heterogeneity can be possibly controlled by genetic interventions. B. subtilis has become a universal microbial cell factory for many industrial products such as enzymes and vitamins. Better enzyme yield would be achieved by reducing cellular heterogeneity in this organism, and that would serve as an asset to the biotech industry. The project will be carried out in the Swammerdam Institute of Life Sciences (SILS) at the University of Amsterdam (UvA), under the supervision of Prof. Leendert Hamoen, one of the leading experts in heterogenic protein expression in the model organism B. subtilis.

Fields of science (EuroSciVoc)

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Keywords

Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)

Programme(s)

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Topic(s)

Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.

Funding Scheme

Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.

HORIZON-TMA-MSCA-PF-EF - HORIZON TMA MSCA Postdoctoral Fellowships - European Fellowships

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Call for proposal

Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.

(opens in new window) HORIZON-MSCA-2022-PF-01

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Coordinator

UNIVERSITEIT VAN AMSTERDAM
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 203 464,32
Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

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