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Label-free multimodal real-time imaging of phage-induced bacterial lysis

Objective

The fast-growing number of multidrug-resistant bacterial strains is one of the biggest threats to public health. 5 million deaths and billions of euros are associated with drug-resistant bacterial infections per year. Lately, bacteriophages have become an alternative way to increasingly failing traditional direct-acting small antibiotic molecules. However, a deep and detailed understanding of the processes connected to bacteriophage-induced bacterial lysis is still missing. This project aims to develop a unique optical-based multimodal imaging technique for high-speed real-time imaging of phage-induced lysis of bacteria. The suggested tailored combination of coherent brightfield microscopy allowing for fast, label-free, and long-term single bioparticle imaging with advanced electro-optic fluorescence lifetime imaging allowing for dynamic molecular proximity sensing will be a worldwide unique prototype. For the first time, the technique will allow for direct label-free tracking of bacteriophages and simultaneous detection of changes in the inner bacterial environment (e.g. change in pH) and bacterial metabolism during all stages of infection. This will provide a great pool of information currently lacking, that will facilitate and accelerate phage and antimicrobial research. The method can be easily adapted for other bacterial or mammalian cells and single-bioparticle and particle studies, finding its place in drug delivery, cell interaction studies, or tracking particles in cells. Due to the uniqueness of the set of information that currently cannot be gained (under the same conditions) with any other technique, we expect the technology to become standard in future life science imaging.

Fields of science (EuroSciVoc)

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Keywords

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Programme(s)

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Topic(s)

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Funding Scheme

Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.

HORIZON-TMA-MSCA-PF-EF - HORIZON TMA MSCA Postdoctoral Fellowships - European Fellowships

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Call for proposal

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(opens in new window) HORIZON-MSCA-2022-PF-01

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Coordinator

UNIVERSITAT WIEN
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 215 841,07
Address
UNIVERSITATSRING 1
1010 WIEN
Austria

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Region
Ostösterreich Wien Wien
Activity type
Higher or Secondary Education Establishments
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

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