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A sonogenetic brain-machine interface for neurosciences and visual restoration

Project description

Sonogenetic brain-machine interface for neurological dysfunction

Brain-machine interfaces typically use multi-electrode arrays, but these systems have limitations. The expression of mechanosensitive proteins in neurons has recently enabled the activation of these neurons by ultrasounds at high frequencies, a technique named sonogenetics. A recent study successfully activated cortical neurons in vivo in rodents using ultrasound with high spatiotemporal resolution at low intensity. The ERC-funded NeuroSonoGene project aims to develop a sonogenetic strategy for activating and inhibiting neuronal networks in brains using ultrasound waves. The project will optimise monogenetic actuators, design technologies for patterned ultrasound stimulation, and demonstrate the efficacy of the approach in controlling the primate visual cortex. The ultimate goal is to create a brain-machine interface for studying neural circuit function and for applications in neurology such as visual restoration.

Objective

Brain-machine interfaces are classically based on multi-electrode arrays. Optogenetics has revolutionized this ability to control neuronal activity through ectopic opsin expression but its application to large brains remains limited because of tissue light absorption and scattering. Ultrasound waves have been proposed for remote neuromodulation, but this approach has remained restricted to low-frequency excitation, to prevent brain tissue overheating, at the expense of spatial resolution. The expression of mechanosensitive proteins in neurons has recently made it possible to achieve the ultrasound (US) activation of neurons at high US frequencies, an approach named “sonogenetic”.
Using the MscL mechanosensitive ionic channel, we recently provided a first proof of concept in rodents that cortical neurons can be activated in vivo by ultrasounds at a high spatiotemporal resolution and low intensity. Here, we propose to develop an integrated sonogenetic strategy for distant activation/inhibition of neuronal networks by ultrasound waves in large primate brains. The project includes: 1) optimizing and diversifying the sonogenetics actuators for safe neuronal control 2) designing technologies for patterned ultrasound stimulations, 3) demonstrating efficacy and high spatiotemporal resolution of the sonogenetic approach in controlling the primate visual cortex. This project is based on a unique synergistic combination of expertise, with a synthetic biologist in ion channel engineering, a neurobiologist with expertise in vision, and a physicist with expertise in ultrasound technologies. These 3 PIs will be supported by an expert in AAV vector mediated delivery. The project outcomes will be a novel brain-machine interface for investigating neural circuit function even deep within large brains through contactless sonogenetics. This sonogenetic technology will open new avenues for treating neurological dysfunction such as restoring vision in blind patients.

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Keywords

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Programme(s)

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Topic(s)

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Funding Scheme

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HORIZON-ERC-SYG - HORIZON ERC Synergy Grants

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Call for proposal

Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.

(opens in new window) ERC-2023-SyG

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Host institution

INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 4 949 998,75
Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

€ 5 760 592,75

Beneficiaries (3)

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