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Restoration of the gut microbiome after delivery by caesarean section to prevent asthma

Project description

Restoring gut health to prevent childhood asthma

Children delivered via caesarean section (CS) face a high risk of developing asthma and allergies by age six. This heightened risk is linked to significant disruptions in gut microbiota, particularly when microbial composition fails to normalise within the first year of life. In this context, the ERC-funded RestoreGut project aims to address this issue by exploring ways to restore the gut microbiome in CS-born infants. Researchers will identify environmental, dietary, and host factors that promote healthy microbial maturation, study the role of the virome, and test faecal microbiota and virome transplantation in newborns. If successful, RestoreGut could pave the way for groundbreaking strategies to prevent asthma and develop targeted microbiota therapies for children with early microbial imbalances.

Objective

Associations between caesarean section (CS) and childhood asthma may be mediated through microbial changes. In 700 children followed prospectively from birth, I recently showed that microbial perturbations after delivery by CS explain the delivery mode-associated risk of developing asthma in the first 6 years of life. CS was associated with more than a doubled risk of later asthma and allergic sensitization as well as immense compositional changes in the gut microbiota. An increased asthma risk was only found in children born by caesarean section, if their microbiome did not normalize by age 1 year, suggesting that a healthy microbial maturation can alleviate the childs increased asthma risk.
Under the hypothesis that asthma risk from CS can be avoided by restoring the early gut microbiome, the proposed project will identify key environmental, dietary and host factors that help restoring a CS perturbed microbiome, map the compositional and functional potential of the CS microbial profiles, evaluate the role of the virome in the CS associations and perform co-cultures of key microbial consortia that may restore a healthy trajectory. Finally, I will perform murine experimental models to validate the findings and perform pilot studies on CS microbiota restoration by fecal microbiota transplantation (FMT) and fecal virome transplantation (FVT) in newborns.
This will be a truly translational research project, with the potential to go from clinical observations to mechanisms based on bioinformatics and microbiological laboratory work and experimental models and a pilot human trial to infer causal relationships with the perspective of developing a potential future intervention strategy.
If successful, this strategy will be groundbreaking for understanding the microbiomes role in asthma development after CS and in the future potentially lead to novel prevention strategies and targeted, efficient microbiota manipulation in children with early microbial perturbations.

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Host institution

REGION HOVEDSTADEN
Net EU contribution
€ 1 999 934,00
Address
KONGENS VAENGE 2
3400 Hillerod
Denmark

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Region
Danmark Hovedstaden Nordsjælland
Activity type
Public bodies (excluding Research Organisations and Secondary or Higher Education Establishments)
Links
Total cost
€ 1 999 934,00

Beneficiaries (1)