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Neuronal Control of Fine Movement Components of Egg Laying in Fruit Fly

Project description

The ventral nerve cord and control of egg laying behaviour in fruit flies

Female insects lay a tremendous number of eggs. An infectious larva population can grow from hundreds to tens of thousands with a month. Furthermore, eggs are the most difficult life stage of insects to kill. Understandings egg laying behaviour and targeting this process could have important impact on mitigating disease spread and crop failure. The EU-funded Neuron to Egg Laying project aims to investigate egg laying in the common fruit fly, Drosophila melanogaster, a well-established eukaryotic model system for studying neuronal function and neuronally expressed genes. The team will focus on the ventral nerve cord and its role in executing egg laying behaviour commands.

Objective

[a short summary to be written shortly]Insect reproduction has a large impact in human well-being through the dissemination of disease and crop failure. Here, we propose to study insect egg laying using Drosophila melanogaster as a model system. We will focus on the ventral nerve cord and its role in executing egg laying behaviour commands. We will take advantage of lines previously generated in the laboratory that label small groups of neurons in the ventral nerve cord to establish how different neurons contribute to egg laying motor elements and explore how they communicate with each other and other identified egg laying neuronal elements to coordinate the execution of the different egg laying steps. For this we will pursue the following aims: Aim1- Development of automated tracking of each element of egg laying behaviour. This will allow a precise, quantitative and fast description of behavioural phenotypes; Aim2- Probe the contribution of different motor-controlling neuronal populations to different elements of egg laying behaviour.Activation phenotypes will be recorded, classified and clustered for each ventral nerve cord line; Aim3 - Establish the connectivity of different neuronal populations.The power of Drosophila genetics will be used determine through anatomical and functional means the connectivity between different sets of neurons, including descending neurons from the brain and other local ventral nerve cord neurons. We will also determine how octopamine modulates the functional connectivity between the neurons.

With the results of this project, we expect to produce important insights into the mechanisms of control and execution of egg laying behaviour.

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Coordinator

FUNDACAO D. ANNA DE SOMMER CHAMPALIMAUD E DR. CARLOS MONTEZ CHAMPALIMAUD
Net EU contribution
€ 172 618,56
Address
AVENIDA BRASILIA, CENTRO DE INVESTIGACAO DA FUNDACAO CHAMPALIMAUD
1400-038 Lisboa
Portugal

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